Preparation and antitumor activity of peptides from Mylopharyngodon piceus muscle
ZHANG Yao1, ZHANG Lu1, LIU Jun1, TU Zongcai1,2*
1(National R&D Center for Freshwater Fish Processing and Engineering Research Center for Freshwater Fish High-value Utilization of Jiangxi Province, Jiangxi Normal University, Nanchang 330022, China) 2(State Key Laboratory of Food Science and Technology, Nanchang University, Nanchang 330047, China)
Abstract: Antioxidant and antitumor peptides were prepared from Mylopharyngodon piceus muscle by two-step enzymolysis and gel chromatography separation. The preparation process was optimized by single factor and optimization experiment with antioxidant activity and degree of hydrolysis as indicators. Gel chromatography was used to separate and purify peptides with different molecular weights. The amino acid composition, 1, 1-diphenyl-2-picrylhydrazyl (DPPH) free radical scavenging capacity and antitumor activity of purified peptide fractions were also analyzed. The results showed that the optimum enzymatic hydrolysis conditions were: alkaline protease and composite protease, pH 8.00, 50 ℃, liquid-to-material ratio of 2∶10 (g∶mL), enzyme concentration of 6 000 U/g and hydrolysis time of 3 h. The half inhibitory concentration (IC50) value for DPPH free radical scavenging of sample prepared under optimal conditions was 9.52 mg/mL. Five peptides fractions (I-V) with molecular weights ranged from 130 to 1 397 Da were isolated by Sephadex G-15. Among which, fraction IV exhibited the highest scavenging ability on DPPH radicals, the IC50 value was 3.17 mg/mL, which was 3 times higher than that of the two-step hydrolysate. Cancer cell inhibition experiment showed that the inhibition rate of fraction IV (10 mg/mL) on HepG2 cells was 92.54%, indicating considerable antioxidant and antitumor activity of M. piceus muscle bioactive peptides. The results enrich the source of antitumor peptides and provides a technical reference for the high-value utilization of freshwater fish resources.
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