Abstract: A simple, rapid and new absorption spectra method for the simultaneous determination of the coexisting colorants—tartrazine and sunset yellow in beverages was developed. In the pH 9.43 Tris-HCl solution, methyl green reacted with tartrazine and sunset yellow to form ionic association complexes. Within the range of 400-750 nm, the tartrazine system produced a characteristic negative absorption peak that can be quantitatively analyzed at the wavelength of 574 nm, and the coexistence pigment sunset yellow did not interfere with the determination of tartrazine. The mass concentration of tartrazine in the range of 0.04-16.0 mg/L had a good linear relationship with the absorbance of the association complex, the apparent molar absorbance coefficient was 4.90×104 L/(mol·cm) with the detection limit of 0.033 mg/L, and the quantitative limit of beverage was 1.80 mg/kg. The sunset yellow system produced a characteristic positive absorption peak that can be quantitatively analyzed at the wavelength of 488 nm, and the coexistence pigment tartrazine did not interfere with the determination of sunset yellow. Moreover, the mass concentration of sunset yellow in the range of 0.04-13.6 mg/L had a good linear relationship with the absorbance of the associative complex, the apparent molar absorbance coefficient was 4.19×104 L/(mol·cm) with the detection limit of 0.028 mg/L, and the quantitative limit of beverage was 1.51 mg/kg. The method was simple, rapid, and it could simultaneously determine the content of coexisting pigments—tartrazine and sunset yellow in the same beverage sample without separation by controlling the acidity of the solution, with the satisfactory results.
江虹,庞向东. 甲基绿吸收光谱法同时测定饮料中的酒石黄和日落黄[J]. 食品与发酵工业, 2021, 47(5): 238-243.
JIANG Hong,PANG Xiangdong. Simultaneous determination of tartrazine and sunset yellow in beverages by methyl green absorption spectrometry[J]. Food and Fermentation Industries, 2021, 47(5): 238-243.
[1] LI R, WANG Y, TAN J, et al.Simultaneous determination of synthetic edible pigments in beverages by titania-based RP-HPLC[J].Arabian Journal of Chemistry, 2020, 13(2):3 875-3 881. [2] CHEN D M, WU M R, XIE S Y, et al.Determination of tartrazine, lutein, capsanthin, canthaxanthin and β-carotene in animal-derived foods and feeds by HPLC method[J].Journal of Chromatographic Science, 2019, 57(5):462-468. [3] 刘剑波, 余莲芳, 朱明扬, 等.固相萃取-高效液相色谱法同时测定蔬菜制品中11种合成着色剂[J].食品研究与开发, 2018, 39(23):141-146. LIU J B, YU L F, ZHU M Y, et al.Simultaneous determination of 11 synthetic colorants in vegetable products by solid phase extraction-high performance liquid chromatography[J].Food Research and Development, 2018, 39(23):141-146. [4] LI Q, LIU W, ZHU X S.Green choline amino acid lonic liquid aqueous two-phase extraction coupled with HPLC for analysis sunset yellow in beverages[J].Food Analytical Methods, 2019, 12(11):2 527-2 534. [5] HOSSEININIA S A R, KAMANI M H, RANI S.Quantitative determination of sunset yellow concentration in soft drinks via digital image processing[J].Journal of Food Measurement and Characterization, 2017, 11(3):1 065-1 070. [6] 高家敏, 钮正睿, 李红霞, 等.高效液相色谱法测定饮料中 12 种水溶性合成着色剂[J].食品安全质量检测学报, 2019, 10(1):135-140. GAO J M, NIU Z R, LI H X, et al.Determination of 12 kinds of water-soluble synthetic colorants in drinks by high performance liquid chromatography[J].Journal of Food Safety and Quality, 2019, 10(1):135-140. [7] 戴玉婷, 杨晋青, 葛淑丽, 等.固相萃取-高效液相色谱法测定饮料中的9种合成着色剂[J].食品与发酵科技, 2019, 55(2):81-84. DAI Y T, YANG J Q, GE S L, et al.Simultaneous determination of nine synthetic colorants in beverages by high performance liquid chromatography with solid phase extraction[J].Food and Fermentation Sciences & Technology, 2019, 55(2):81-84. [8] TAHTAISLEYEN S, GORDUK O, SAHIN Y.Electrochemical determination of tartrazine using a graphene/poly(L-phenylalanine) modified pencil graphite electrode[J].Analytical Letters, 2020, 53(11):1 683-1 703. [9] BAYTAK A K, AKBA E, ASLANOGLU M.A novel voltammetric platform based on dysprosium oxide for the sensitive determination of sunset yellow in the presence of tartrazine[J].Analytica Chimica acta, 2019, 1087:93-103. [10] CHEBOTAREV A, KOICHEVA A, BEVZIUK K, et al.Simultaneous determination of sunset yellow and tartrazine in soft drinks on carbon-paste electrode modified by silica impregnated with cetylpyridinium chloride[J].Journal of Food Measurement and Characterization, 2019, 13(3):1 964-1 972. [11] NOVIANDRI I, RAMINAS A.The effect of tartrazine in voltammetric determination of rhodamin B with molecularly imprinted poly(3-aminophenol) modified carbon electrode[J].Key Engineering Materials, 2019, 4 902:107-112. [12] ARVAND M, ZEINOLABEDINPOUR G.Determination of sunset yellow in foodstuffs by surface modification of nonconductive polyester of polyvinyl alcohol sheet used as overhead projector film[J].Food Analytical Methods, 2019, 12(8):1 858-1 870. [13] DEROCO P B, MEDEIROS P R A, ROCHA-FILHO P R C, et al.Simple flow injection analysis system coupled to multiple-pulse amperometry and a boron-doped diamond electrode for the simultaneous determination of sunset yellow and aspartame[J].Chem Electro Chem, 2020, 7(8):1 943-1 950. [14] TRAN Q T, PHUNG T T, NGUYEN Q T, et al.Highly sensitive and rapid determination of sunset yellow in drinks using a low-cost carbon material-based electrochemical sensor[J].Analytical and Bioanalytical Chemistry, 2019, 411(28):7 539-7 549. [15] GHANBARI K, ROUSHANI M, FARZADFAR F, et al.Developing a four-dimensional voltammetry as a powerful electroanalytical methodology for simultaneous determination of three colorants in the presence of an uncalibrated interference[J].Chemometrics and Intelligent Laboratory Systems, 2019, 189:27-38. [16] YANG X P, LUO N, TAN Z J, et al.A fluorescence probe for tartrazine detection in foodstuff samples based on fluorescence resonance energy transfer[J].Food Analytical Methods, 2017, 10(5):1 308-1 316. [17] 杨彩玲, 赵国虎, 张鸣, 等.氮掺杂碳量子点荧光猝灭法测定柠檬黄[J].化学研究与应用, 2019, 31(7):1 293-1 298. YANG C L, ZHAO G H, ZHANG M, et al.Determination of tartrazine based on fluorescent quenching of nitrogen doped carbon quantum dot[J].Chemical Research and Application, 2019, 31(7):1 293-1 298. [18] TÜLAY O, ERIFE T, AFAK C, et al.Determination of color additive tartrazine (E102) in food samples after dispersive solid phase extraction with a zirconium-based metal-organic framework (UiO-66(Zr)-(COOH)2)[J].Food Additives & Contaminants:Part A, 2020, 37(5):731-741. [19] DUDKINA A A, VOLGINA T N, SARANCHINA N V, et al.Colorimetric determination of food colourants using solid phase extraction into polymethacrylate matrix[J].Talanta, 2019, 202:186-189. [20] 孙延春, 敬铭.分光光度法测定饮料中色素含量[J].四川理工学院学报(自然科学版), 2012, 25(5):25-28. SUN Y C, JING M.Determination of pigments in beverage by ultraviolet spectrophotometry[J].Journal of Sichuan University of Science & Engineering(Natural Science Edition), 2012, 25(5):25-28. [21] 李紫薇, 袁阳, 贾风勤.分光光度法同时测定饮料中的苋菜红和柠檬黄[J].伊犁师范学院学报(自然科学版), 2010(4):30-32. LI Z W, YUAN Y, JIA F Q.Spectrophotometric determination of amaranth and tartrazine in drinks[J].Journal of Yili Normal University (Natural Science Edition), 2010(4):30-32.