Abstract: A simple, rapid and new absorption spectra method for the simultaneous determination of the coexisting colorants—tartrazine and sunset yellow in beverages was developed. In the pH 9.43 Tris-HCl solution, methyl green reacted with tartrazine and sunset yellow to form ionic association complexes. Within the range of 400-750 nm, the tartrazine system produced a characteristic negative absorption peak that can be quantitatively analyzed at the wavelength of 574 nm, and the coexistence pigment sunset yellow did not interfere with the determination of tartrazine. The mass concentration of tartrazine in the range of 0.04-16.0 mg/L had a good linear relationship with the absorbance of the association complex, the apparent molar absorbance coefficient was 4.90×104 L/(mol·cm) with the detection limit of 0.033 mg/L, and the quantitative limit of beverage was 1.80 mg/kg. The sunset yellow system produced a characteristic positive absorption peak that can be quantitatively analyzed at the wavelength of 488 nm, and the coexistence pigment tartrazine did not interfere with the determination of sunset yellow. Moreover, the mass concentration of sunset yellow in the range of 0.04-13.6 mg/L had a good linear relationship with the absorbance of the associative complex, the apparent molar absorbance coefficient was 4.19×104 L/(mol·cm) with the detection limit of 0.028 mg/L, and the quantitative limit of beverage was 1.51 mg/kg. The method was simple, rapid, and it could simultaneously determine the content of coexisting pigments—tartrazine and sunset yellow in the same beverage sample without separation by controlling the acidity of the solution, with the satisfactory results.
江虹,庞向东. 甲基绿吸收光谱法同时测定饮料中的酒石黄和日落黄[J]. 食品与发酵工业, 2021, 47(5): 238-243.
JIANG Hong,PANG Xiangdong. Simultaneous determination of tartrazine and sunset yellow in beverages by methyl green absorption spectrometry[J]. Food and Fermentation Industries, 2021, 47(5): 238-243.
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