Comparative analysis of squalene and the characteristic indexes in pumpkin seed oils
YANG Xuefang1, ZHANG Jiguang2,3,4, WU Wanfu2,3,4, LYU Shidong2,3,4*
1(Scientific and technological achievements incubation center, Kunming Medical University, Kunming 650500, China) 2(Kunming Grain and Oil and Feed Product Quality Inspection Center, Kunming 650118, China) 3(Yunnan Province Grain and Oil Products Quality Supervision and Inspection Station, Kunming 650118, China) 4(Yunnan Kunming National Grain Quality Testing Station, Kunming 650118, China)
Abstract: The content of squalene and characteristics indexes (iodine value, saponification value, relative density, refractive index, fatty acid composition) of 10 pumpkin seed oils which from different regions in China were analyzed by gas chromatography(GC), gas chromatography-mass spectrometry(GC-MS), and chemical titration method, etc. And the contents of squalene were compared with other special woody oils. The results showed that the relative density of pumpkin seed oils ranged between 0.918 8 and 0.921 5, and the refractive index was 1.456 0-1.466 9. Moreover, iodine value was 108.4-116.2 g/100 g and saponification value was 188.9-192.4 mg/g. A total of 14 fatty acid components were detected, mainly including linoleic acid and oleic acid in which linoleic acid content were 44.7%-55.5%, oleic acid content was 23.8%-33.1%, the unsaturated fatty acid content in 10 pumpkin seed oils were higher than 78.2%. Furthermore, squalene content in pumpkin seed oils was significantly higher than Camellia seed oils, macadamia oils, walnut oils and avocado oils which reached 310-4 446 mg/kg. After comprehensive consideration oil color, squalene contents and principal component analysis(PCA), it was speculated that the great difference of squalene content could be related to the preparation process of oil, squalene was lost in some pumpkin seed oils during deep processing. In conclusion, pumpkin seed oil has high nutritional value, which can provide certain data support for the deep processing and development of pumpkin seed, and improve the comprehensive utilization value of pumpkin seed.
杨学芳,张继光,吴万富,等. 南瓜籽油中角鲨烯含量及特征指标比较[J]. 食品与发酵工业, 2021, 47(5): 217-223.
YANG Xuefang,ZHANG Jiguang,WU Wanfu,et al. Comparative analysis of squalene and the characteristic indexes in pumpkin seed oils[J]. Food and Fermentation Industries, 2021, 47(5): 217-223.
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2021, Vol. 47 
