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食品与发酵工业  2021, Vol. 47 Issue (7): 225-231    DOI: 10.13995/j.cnki.11-1802/ts.025536
  分析与检测 本期目录 | 过刊浏览 | 高级检索 |
QDA和GC-MS结合PLSR分析宁红茶中的风味物质
岳翠男1,2, 秦丹丹3, 蔡海兰1,2, 李琛1,2, 王治会1,2, 李文金1,2, 杨普香1,2*, 吴华玲3*
1(江西省蚕桑茶叶研究所,江西 南昌,330202)
2(江西省茶叶质量与安全控制重点实验室,江西 南昌,330202)
3(广东省农业科学院 茶叶研究所,广东 广州, 510640)
Flavor compounds in Ning black tea by QDA and GC-MS combined with PLSR
YUE Cuinan1,2, QIN Dandan3, CAI Hailan1,2, LI Chen1,2, WANG Zhihui1,2, LI Wenjin1,2, YANG Puxiang1,2*, WU Hualing3*
1(Jiangxi Sericulture and Tea Research Institute,Nanchang 330202,China)
2(Jiangxi Key Laboratory of Tea Quality and Safety Control,Nanchang 330202,China)
3(Tea Research Institute,Guangdong Academy of Agricultural Science,Guangzhou 510640,China)
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摘要 为了探究宁红茶香气感官特征、挥发性化合物组成及其分子感官基础,采用定量描述分析(quantitative description analysis,QDA)与顶空固相微萃取-气质联用(headspace-solid phase microextraction-gas chromatography-mass spectrometry,HS-SPME-GC-MS)相结合,对8份典型宁红茶的香气特征及挥发性化合物进行分析,之后利用偏最小二乘法(partial least squares regression,PLSR)筛选对其香气特征具有重要贡献的挥发性成分。结果表明,宁红茶香气可由筛选出的8个香气术语及其定义进行表征,其香气特征以果香为主,甜香和花香为辅,其中花香和甜香相关性大,焙烤香和果香相关性大,辛香与清香相关性大。鉴定出74个共有挥发性成分,以醇类含量最高,酯类、酸类、醛类、酮类次之,各宁红样品之间的挥发性成分含量差异性较大。最终筛选出16个对宁红茶香气特征具有重要贡献的化合物,分别为香叶醇、反式-橙花叔醇、苯乙醇、苯甲醛、芳樟醇、α-紫罗酮、β-水芹烯、罗勒烯、β-紫罗酮、橙花醇、棕榈酸乙酯、3-糠醛、苯甲醇、己酸、反式-3-己烯酸和水杨酸甲酯。实验结果为进一步研究宁红茶香气物质前体、生化过程和品质定向调控提供了基础。
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岳翠男
秦丹丹
蔡海兰
李琛
王治会
李文金
杨普香
吴华玲
关键词:  宁红茶  香气特征  挥发性物质  气质联用  偏最小二乘法    
Abstract: To explore the sensory characteristics, volatile compounds composition and molecular sensory basis of Ning black tea.In this study, QDA combined with HS-SPME-GC-MS was used to analyze the aroma characteristics and volatile compounds of eight typical Ning black tea samples, and then PLSR was used to screen the volatile components which had an important contribution to the aroma characteristics.The results showed that the aroma of Ning black tea could be characterized by eight aroma terms and their definitions.The aroma characteristics of Ning black tea were mainly fruity, supplemented by sweet-smell and floral.Among them, the correlation between floral and sweet-smell was large, the correlation between roasted and fruity was large, and the correlation between spicy and freshly was large.Seventy five common volatile components were identified, among which alcohols were the highest, followed by esters, acids, aldehydes and ketones.The differences in volatile components among Ning black tea samples were significant.Finally, sixteen compounds which contributed significantly to the aroma characteristics of Ningblack tea were identified, namely, trans nerolidol, phenylethanol, benzaldehyde, linalool, α-ionone, β-carvone, ocimene, β-ionone, nerol, ethyl palmitate, 3-furfural, benzyl alcohol, hexanoic acid, trans-3-hexenoic acid and methyl salicylate.This experiment provides a basis for further study on aroma precursor, biochemical process and quality control of Ning black tea.
Key words:  Ning black tea    aroma characteristics    volatile components    GC-MS    PLSR
收稿日期:  2020-08-31      修回日期:  2020-09-23           出版日期:  2021-04-15      发布日期:  2021-05-20      期的出版日期:  2021-04-15
基金资助: 江西省现代农业产业技术体系建设专项(JXARS-02);国家现代农业产业技术体系建设专项(CARS-19);江西省茶叶质量与安全控制重点实验室茶叶加工课题(20192BCD40007);江西省重点研发计划项目(20202BBF63004)
作者简介:  硕士,助理研究员(杨普香研究员和吴华玲研究员为共同通迅作者,E-mail:jxypx@163.com;wuhualing@163.com)
引用本文:    
岳翠男,秦丹丹,蔡海兰,等. QDA和GC-MS结合PLSR分析宁红茶中的风味物质[J]. 食品与发酵工业, 2021, 47(7): 225-231.
YUE Cuinan,QIN Dandan,CAI Hailan,et al. Flavor compounds in Ning black tea by QDA and GC-MS combined with PLSR[J]. Food and Fermentation Industries, 2021, 47(7): 225-231.
链接本文:  
http://sf1970.cnif.cn/CN/10.13995/j.cnki.11-1802/ts.025536  或          http://sf1970.cnif.cn/CN/Y2021/V47/I7/225
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