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食品与发酵工业  2021, Vol. 47 Issue (7): 102-108    DOI: 10.13995/j.cnki.11-1802/ts.025591
  研究报告 本期目录 | 过刊浏览 | 高级检索 |
酱香型白酒醇类活性成分抗幽门螺杆菌损伤胃黏膜上皮细胞活性的研究
罗丹, 罗强, 刘杰, 刘志刚*
(深圳大学 医学部,广东 深圳,518000)
Active alcohols in Maotai-flavor Baijiu against the damages in gastric mucosal epithelial cells induced by Helicobacter pylori
LUO Dan, LUO Qiang, LIU Jie, LIU Zhigang*
(Health Science Center,Shenzhen University,Shenzhen 518000,China)
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摘要 为研究酱香型白酒醇类活性成分(active alcohols, AAs)抑制幽门螺杆菌(Helicobacter pylori)损伤胃黏膜上皮(GES-1)细胞活性及潜在的机制,检测了AAs抑制H.pylori的最小半数抑菌浓度(50% minimun inhibitory concentration, MIC50)及苯乙醇(AA-29)抑制H.pylori损伤GES-1细胞活性;分析了AA-29对H.pylori脲酶活性的影响;通过RT-PCR分析了AA-29对H.pylori脲酶基因簇(ureAureBureEureHureInixA)、外膜蛋白基因(babA)和毒力基因(cagAvacA)转录的影响;采用Western blotting检测了AA-29对cagAvacA表达的影响;ELISA及流式检测AA-29对H.pylori诱导的GES-1细胞TNF-α、IL-1β、IL-6及细胞凋亡的影响。结果表明:AA-29活性最佳,可显著抑制H.pylori生长,其MIC50(72 h)为105.2 μg/mL;100 μg/mL的AA-29可显著抑制H.pylori的脲酶活性;RT-PCR结果显示,AA-29可抑制H.pylori脲酶基因簇中的ureAureBnixAbabAcagA的转录及cagA的表达;AA-29可抑制H.pylori诱导的GES-1细胞TNF-α、IL-1β、IL-6分泌及细胞凋亡。酱香型白酒中的醇类化合物具有一定的抗H.pylori活性,其中AA-29可通过抑制H.pylori脲酶相关基因的表达并抑制H.pylori脲酶活性;AA-29可降低H.pylori babAcagA转录及cagA的表达以减轻H.pylori对胃黏膜上皮细胞的损伤。
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罗丹
罗强
刘杰
刘志刚
关键词:  酱香型白酒  幽门螺杆菌  脲酶  毒力基因    
Abstract: To study the activity and the underlying mechanism of active alcohols (AA) in Maotai-flavor Baijiu against the damages in gastric mucosal epithelial cells induced by Helicobacter pylori, the minimum half inhibitory concentration (MIC50) of AAs against the H. pylori and the inhibition of H.pylori damage to GES-1 cells by phenethyl alcohol (AA-29) were detected. The influence of AA-29 on H. pylori urease activity was analyzed by ELISA. Furthermore, RT-PCR was used to analyze the transcription of H. pylori urease gene clusters (ureA, ureB, ureE, ureH, ureI and nixA), outer membrane protein genes (babA) and virulence genes (cagA and vacA) after the AA-29 treatments. Western blotting was used to detect the effect of AA-29 on the expression of cagA and vacA. ELISA and flow cytometric detection were used to detect the effect of AA-29 on TNF-α, IL-1β, IL-6 expressions and apoptosis of GES-1 cells induced by H. pylori. Results showed that AA-29 performed the most protective activity and significantly inhibited the growth of H. pylori. The MIC50 (72 h) of AA-29 was 105.2 μg/mL; AA-29 (100 μg/mL) significantly inhibited the urease activity of H. pylori; RT-PCR results showed that AA-29 inhibited the transcription of H. pylori urease gene clusters (ureA, ureB, nixA) and outer membrane protein gene (babA) and virulence gene (cagA). AA-29 also inhibited the expression of cagA. AA-29 inhibited H. pylori-induced TNF-α, IL-1β, IL-6 secretion and GES-1 cell apoptosis. The alcohols in Maotai-flavor Baijiu performed anti-H. pylori activity, and AA-29 inhibited the expression of H. pylori urease related genes and inhibited the H. pylori urease activity. AA-29 attenuated the damages induced by H.pylori in gastric epithelial cells by inhibiting the H.pylori babA, cagA transcription and cagA expression.
Key words:  Moutai-flavor Baijiu    Helicobacter pylori    urease    virulence gene
收稿日期:  2020-09-06      修回日期:  2020-10-26           出版日期:  2021-04-15      发布日期:  2021-05-20      期的出版日期:  2021-04-15
作者简介:  硕士研究生(刘志刚教授为通讯作者,Email:zhigangliua66@126.com)
引用本文:    
罗丹,罗强,刘杰,等. 酱香型白酒醇类活性成分抗幽门螺杆菌损伤胃黏膜上皮细胞活性的研究[J]. 食品与发酵工业, 2021, 47(7): 102-108.
LUO Dan,LUO Qiang,LIU Jie,et al. Active alcohols in Maotai-flavor Baijiu against the damages in gastric mucosal epithelial cells induced by Helicobacter pylori[J]. Food and Fermentation Industries, 2021, 47(7): 102-108.
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http://sf1970.cnif.cn/CN/10.13995/j.cnki.11-1802/ts.025591  或          http://sf1970.cnif.cn/CN/Y2021/V47/I7/102
[1] 崔利.酱香型白酒中吡嗪类化合物的生成途径及环节[J].酿酒,2007(5):39-40.CUI L.Biosynthetic pathways and steps of pyrazine compounds in Moutai-flavour Baijiu[J].Liquor Making, 2007(5):39-40.
[2] 郑岩,汤庆莉,吴天祥,等.GC-MS法建立贵州茅台酒指纹图谱的研究[J].中国酿造,2008(9):78-80.ZHENG Y,TANG Q l,WU T X,et al.Study on fingerprint construction of Kweichow Moutai liquor by GC-MS[J].China Brewing,2008(9):78-80.
[3] 庄名扬.酱香型白酒中微量成分的生理活性[J].酿酒,2006(6):109-110.ZHUANG M Y.Physiological activity of trace components in Maotai flavor Baijiu[J].Liquor Making,2006(6):109-110.
[4] SUN Q,XIANG P,SHEN B,et al.Identification of Kweichow Moutai liquor by gas chromatography-mass spectrometry fingerprint.[J].Chinese Journal of Chromatography,2010,28(9):833.
[5] 罗强,刘杰,刘志刚.酱香型白酒中活性成分的抗氧化活性[J].食品与发酵工业,2019,45(23):84-89.LUO Q,LIU J,LIU Z G.Antioxidant activity of active components in Maotai-flavor liquor[J].Food and Fermentation Industries,2019,45(23):84-89.
[6] 申莉.川芎嗪注射液临床应用研究进展[J].中国乡村医药,2008(4):48-49.SHEN L.Progress in clinical application of ligustrazine injection[J].Chinese Journal of Rural Medicine,2008(4):48-49.
[7] MLADENOVA I,DURAZZO M.Transmission of Helicobacter pylori[J].Minerva Gastroenterologicae Dietologica,2018,64(3):251-254.
[8] PARK D,PARK J H,KIM H,et al.M1117 The effect of probiotics and mucoprotective agents on PPI-based Triple therapy for eradication of Helicobacter pylori[J].Gastroenterology,2010,138(5):S-335.
[9] 连大卫,许艺飞,扶丽君,等.广藿香醇对幽门螺杆菌酸抵抗能力的影响及其机制探讨[J].山东医药,2017,57(34):16-19.LIAN D W,XU Y F,FU L J,et al.Effect of patchouli alcohol on acid resistance of Helicobacter pylori[J].Shandong Medical Journal,2017,57(34):16-19.
[10] 王琼,杨杰,潘科,等.幽门螺杆菌不同运送条件及培养基分离效果的比较[J].世界华人消化杂志,2016,24(8):1 241-1 246.WANG Q,YANG J,PAN K,et al.Effect of different transport conditions and media on Helicobacter pylori isolation[J].World Chinese Journal of Digestology,2016,24(8):1 241-1 246.
[11] 顾性初.用于筛选天然产物中脲酶抑制剂的脲酶活性测试方法的建立[J].中国医药工业杂志,1995(11):487-489.GU X C.Establishment of a urease assay for screening urease inhibitors in natural product[J].Chinese Journal of Pharmaceuticals,1995(11):487-489.
[12] 宋鑫,陈惠新,陈洁,等.幽门螺杆菌细胞毒素相关基因A对人胃腺癌AGS细胞生物学行为的影响[J].中华肿瘤杂志,2008(5):339-342.SONG X,CHEN H X,CHEN J,et al.Effect of Helicobacter pylori-encoded CagA on biological behavior of gastric adenocarcinoma cells in vitro[J].Chinese Journal of Oncology,2008(5):339-342.
[13] 张雁,张姝,罗昭逊,等.中药头花蓼对幽门螺杆菌CagA及VacA表达的影响[J].贵阳医学院学报,2015,40(5):455-458.ZHANG Y,ZHANG S,LUO Z X,et al.Effect of Chinese medicine polygonum capitatum on expression of Helicobacter pylori CagA and VacA[J].Journal of Guizhou Medical University,2015,40(5):455-458.
[14] 高俊,陈慧,詹轶群,等.病原微生物毒力因子与宿主细胞信号通路相互作用研究进展[J].生物技术通讯,2016,27(5):705-710.GAO J,CHEN H,ZAN Y Q,et al.Progress in the interaction of pathogenic microbes virulence factors with host cell signalling[J].Letters in Biotechnology,2016,27(5):705-710.
[15] 易健,舒徐,吕静,等.ROS在幽门螺杆菌对胃上皮细胞DNA损伤中的作用[J].世界华人消化杂志,2014,22(35):5 393-5 399.YI J,SHU X,LYU J,et al.Role of ROS in DNA damage caused by Helicobacter pylori in gastric epithelial cells[J]World Chinese Journal of Digestology,2014,22(35):5 393-5 399
[16] 王静,王蔚虹.幽门螺杆菌定植相关致病因子研究进展[J].中国病原生物学杂志,2010,5(2):130-133.WAMG J,WANG W H.Pathogenic factors related to Helicobacter pylori colonization[J].Journal of Pathogen Biology,2010,5(2):130-133.
[17] 耿春雨,马智军,胡继科,等.幽门螺杆菌毒力因子及其致病机制研究进展[J].山东医药,2018,58(7):108-110.GENG C Y,MA Z J,HU J K,et al.Research progress on virulence factors and pathogenic mechanism of Helicobacter pylori[J].Shandong Medical Journal,2018,58 (7):108-110.
[18] 汪强武,王启之,于东红,等.幽门螺杆菌及其细胞毒素相关蛋白A与胃癌形成的关系及可能机制[J].中国组织化学与细胞化学杂志,2013,22(3):260-265.WANG Q W,WAMG Q Z,YU D H,et al.Relationship and possible mechanism between Helicobacter pylori and cytotoxin-associated protein A with aastric carcinoma[J].Chinese Journal of Histochemistry and Cytochemistry,2013,22(3):260-265.
[19] 李晓姣,赵圣国,郑楠,等.细菌脲酶蛋白复合物及其活化机制[J].生物工程学报,2019,35(2):204-215.LI X J,ZHAO S G,ZHENG N,et al.Progress in bacterial urease complexes and their activation mechanisms[J].Chinese Journal of Biotechnology,2019,35(2):204-215.
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