Abstract: L-Isoleucine belongs to the branched chain amino acids. It is also a glucogenic and ketogenic amino acid. As one of the eight essential amino acids for human, it has been widely used in medicine, cosmetics and food industries. L-Isoleucine is industrially produced via fermentation method by Corynebacterium glutamicum and Escherichia coli. In this study, the biosynthesis pathway and metabolic regulation mechanisms of L-isoleucine in C. glutamicum and E. coli were compared. Despite the similarity of the two pathways, there are differences in the diversity and regulation modes of key enzymes. Furthermore, metabolic engineering strategies for production of L-isoleucine and its derivatives were overviewed on removing the feedback inhibition of key enzymes, blocking or weakening the branched pathways, modifying the transport system and enhancing the supply of co-factors, aiming to provide references for breeding production strain.
芦楠,李宇虹,陈宁,等. L-异亮氨酸及其衍生物代谢工程研究进展[J]. 食品与发酵工业, 2021, 47(9): 307-313.
LU Nan,LI Yuhong,CHEN Ning,et al. Advances on metabolic engineering of L-isoleucine and its derivatives[J]. Food and Fermentation Industries, 2021, 47(9): 307-313.
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2021, Vol. 47 
