Abstract: To improve the comprehensive utilization rate of Liangping pomelo peel, a single factor experiment and response surface methodology were used to optimize the enzymatic assisted extraction process parameters of polysaccharide from Liangping pomelo peel.The structure of it was characterized by liquid chromatography-mass spectrometry, ultraviolet spectrum, infrared spectroscopy, and scanning electron microscope, and the antioxidant activity in vitro was investigated.The optimum extraction conditions were as follows:the liquid-solid ratio was 25∶1 (mL∶g), the pH was 6.0, the enzymolysis time was 90 min, and the enzymolysis temperature was 50 ℃.Under these conditions, the extraction rate of polysaccharide was 5.46%.The monosaccharide composition showed that it was mainly composed of ribose (1.18%), glucuronic acid (1.32%), mannose (4.26%), rhamnose (4.72%), glucose (11.46%), galactose (21.48%), galacturonic acid (23.67%) and arabinose (31.90%);the results of UV and IR spectra showed that it was acidic polysaccharide with pyranose ring and linked by β-glucoside bond, and contained no protein and nucleic acid.The results of the scanning electron microscope showed that it had lamellar and fibrous structures.In addition, the results of in vitro antioxidant experiment showed that the antioxidant activities was dose-dependent in a certain concentration range.And the IC50of DPPH radical scavenging rate, hydroxyl radical scavenging rate and superoxide anion radical scavenging rate were 1.71,1.43 and 5.37 mg/mL, respectively.This study can provide a reference for the development and utilization of Liangping pomelo peel.
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