Abstract: Single-factor experiment and response surface methodology were used to optimize the ultrasonic-assisted aqueous two-phase extraction of polysaccharides from Potentilla anserina L. Four key factors including ethanol concentration, ammonium sulfate concentration, solid-liquid ratio and ultrasonic power were selected by one-factor-at-a-time experiments. The optimum conditions of ultrasonic-assisted aqueous two-phase extraction of polysaccharides from Potentilla anserina L. were as follows: Ultrasonic power at 240 W with 31% ethanol volume and 19% ammonium sulfate, the extraction yield of polysaccharides from Potentilla anserina L. reached (9.035±0.119)%. Infrared spectrum analysis confirmed that the extracts were polysaccharides. Potentilla anserina polysaccharide was composed of rhamnose, mannose, glucose and galactose, with the molar ratio of 1.00∶1.69∶10.61∶2.66. The in vitro activity results showed that the antioxidant activity of polysaccharides extracted by ultrasonic-assisted two-phase extraction was better than that of traditional water extraction. Ultrasonic assisted aqueous two-phase technology was a reliable and efficient method for polysaccharide extraction.
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