Abstract: Single-factor experiment and response surface methodology were used to optimize the ultrasonic-assisted aqueous two-phase extraction of polysaccharides from Potentilla anserina L. Four key factors including ethanol concentration, ammonium sulfate concentration, solid-liquid ratio and ultrasonic power were selected by one-factor-at-a-time experiments. The optimum conditions of ultrasonic-assisted aqueous two-phase extraction of polysaccharides from Potentilla anserina L. were as follows: Ultrasonic power at 240 W with 31% ethanol volume and 19% ammonium sulfate, the extraction yield of polysaccharides from Potentilla anserina L. reached (9.035±0.119)%. Infrared spectrum analysis confirmed that the extracts were polysaccharides. Potentilla anserina polysaccharide was composed of rhamnose, mannose, glucose and galactose, with the molar ratio of 1.00∶1.69∶10.61∶2.66. The in vitro activity results showed that the antioxidant activity of polysaccharides extracted by ultrasonic-assisted two-phase extraction was better than that of traditional water extraction. Ultrasonic assisted aqueous two-phase technology was a reliable and efficient method for polysaccharide extraction.
白世俊, 包锦渊, 李军乔.不同地区蕨麻中多糖含量的测定[J].湖北农业科学, 2012, 51(19):4 361-4 363;4 373.BAI S J, BAO J Y, LI J Q.Determination of the polysaccharide content of Potentilla anserina in different regions[J].Hubei Agricultural Sciences, 2012, 51(19):4 361-4 363;4 373.
[2]
拉本. 藏药蕨麻的民族植物学研究[J].中央民族大学学报(自然科学版), 2013, 22(3):12-17.LA B.Ethnobotany study on the Tibetan medicine Potentilla anserine[J].Journal of MUC (Natural Sciences Edition), 2013, 22(3):12-17.
[3]
TOSHIO M, KIYOFUMI N, KATSUYA I, et al.Hepatoprotective triterpenes from traditional Tibetan medicine Potentilla anserina[J].Phytochemistry, 2014, 102:169-181.
[4]
QIN X J, LI L Z, Lu Q, et al.Neuroprotection of n-Butanol extract from roots of Potentilla anserina on hypoxic injury in primary hippocampal neurons[J].Chinese Herbal Medicines, 2012, 4(3):195-200.
[5]
ZHAO Y L, CAI G M, HONG X, et al.Anti-hepatitis B virus activities of triterpenoid saponin compound from Potentilla anserina L[J].Phytomedicine, 2008, 15(4):253-258.
[6]
CHEN J R, YANG Z Q, HU T J, et al.Immunomodulatory activity in vitro and in vivo of polysaccharide from Potentilla anserina[J].Fitoterapia, 2010, 81(8):1 117-1 124.
[7]
高丹丹, 杨璐璐, 祁高展.响应面法优化蕨麻多糖的提取工艺[J].天然产物研究与开发, 2015, 27(6):1 099-1 104.GAO D D, YANG L L, QI G Z.Optimization of extraction conditions of polysaccharide from Potentilla anserina by response surface methodology[J].Natural Product Research and Development, 2015, 27:1 099-1 104.
[8]
陈炼红, 张梦苑, 伍红, 等.复合酶法提取蕨麻多糖及其抗氧化活性研究[J].西南民族大学学报(自然科学版), 2015, 41(4):407-411.CHEN L H, ZHANG M Y, WU H, et al.Extraction and antioxidant activities of polysaccharides from Potentilla anserina with combined-enzyme method[J].Journal of Southwest University for Nationalities (Natural Science Edition), 2015, 41(4):407-411.
[9]
范晓伟, 冉露, 郭箐, 等.离子液体结合超声辅助和双水相萃取提取分离柑橘果皮中黄酮类化合物[J].食品科学, 2020, 41(10):265-271.FAN X W, RAN L, GUO Q, et al.Extraction and separation of flavonoids from citrus peel by successive ultrasound-assisted with ionic liquids and aqueous two-phase extraction[J].Food Science, 2020, 41(10):265-271.
[10]
JHONATAS R B, MAUR'ICIO M S F.Polysaccharides of mushroom pleurotus spp:New extraction techniques, biological activities and development of new technologies[J].Carbohydrate Polymers, 2019,19:1 197-1 207.
[11]
张鉥孟, 陈美珍.乙醇-硫酸铵双水相体系萃取坛紫菜多糖[J].食品科学, 2014, 35(22):46-49.ZHANG S M, CHEN M Z.Extraction of polysaccharides from Porphyra haitanensis using ethanol-ammonium sulfate aqueous two-phase system[J].Food Science, 2014, 35(22):46-49.
[12]
DAYEON R, EUNMI K.Application of response surface methodology to acidified water extraction of black soybeans for improving anthocyanin content, total phenols content and antioxidant activity[J].Food Chemistry, 2018, 261:260-266.
[13]
JAWHAR H, KHAOULA M H, DIDIER L C, et al.Characterization, antioxidant and antiglycation properties of polysaccharides extracted from the medicinal halophyte Carpobrotus edulis L[J].International Journal of Biological Macromolecules, 2018, 107:833-842.
[14]
BALASUBRAMANIAN B, ILAVENIL S, Al-DHABI N A, et al.Isolation and characterization of Aspergillus sp.for the production of extracellular polysaccharides by response surface methodology[J].Saudi Journal of Biological Sciences, 2019, 26:449-454.
[15]
XUE H Y, LI J R, LIU Y, et al.Optimization of the ultrafiltration-assisted extraction of Chinese yam polysaccharide using response surface methodology and its biological activity[J].International Journal of Biological Macromolecules, 2019, 121:1 186-1 193.
[16]
杨娜, 王鸿飞, 许凤.蕨麻多糖提取及抗氧化活性研究[J].中国食品学报, 2014, 14(2):60-66.YANF N, WANG H F, XU F.Study on extraction of polysaccharides and antioxidant Activity of Potentilla ancerina L.polysaccharide[J].Journal of Chinese Institute of Food Science and Technology, 2014, 14(2):60-66.
[17]
KACZMARSKA K, GRABOESKA B, SPYCHAJ T, et al.Effect of microwave treatment on structure of binders based on sodium carboxymethyl starch:FT-IR, FT-Raman and XRD investigations[J].Spectrochimica Acta Part A:Molecular and Biomolecular Spectroscopy, 2018, 199:387-393.
[18]
SATHISHKUMAR R, ANANTHAN G S, SENTHIL L, et al.Structural characterization and anticancer activity of extracellular polysaccharides from ascidian symbiotic bacterium Bacillus thuringiensis[J].Carbohydrate Polymers, 2018, 190:113-120.
[19]
PALANIPPAN S, MEIVELU M, SADHASIVAM S, et al.Isolation and chemical characteristics of rhamnose enriched polysaccharide from Grateloupia lithophila[J].Carbohydrate Polymers, 2018, 195:486-494.
[20]
GUO J, WANG J L, SONG S, et al.Sphallerocarpus gracilis polysaccharide protects pancreatic beta-cells via regulation of the bax/bcl-2, caspase-3, pdx-1 and insulin signalling pathways[J].International Journal of Biological Macromolecules, 2016, 93:829-836.
[21]
SATHISHKUMAR R S, ANANTHAN G, SENTHIL S L, et al.Structural characterization and anticancer activity of extracellular polysaccharides from ascidian symbiotic bacterium Bacillus thuringiensis[J].Carbohydrate Polymers, 2018, 190:113-120.
[22]
MENG L, SUN S, LI R, et al.Antioxidant activity of polysaccharides produced by Hirsutella sp.and relation with their chemical characteristics[J].Carbohydrate Polymers, 2015, 117:452-457.
[23]
JING X Y, MAO D B, GENG L J.Medium optimization, molecular characterization, and bioactivity of expolysaccharides from Pleurotus eryngii[J].Archives of Microbiology, 2013, 195:749-757.
[24]
BHAT A H, DAR K B, ANEES S, et al.Oxidative stress, mitochondrial dysfunction and neurodegenerative diseases;a mechanistic insight[J].Biomedicine & Pharmacotherapy, 2015, 74:101-110.
[25]
ABDOH T, ALI A K, ULLAH I A, et al.Antioxidant effects and mechanism of silymarin in oxidative stress induced cardiovascular diseases[J].Biomedicine & Pharmacotherapy, 2018, 102:689-698.
[26]
OLATUNJI O J, YAN F, OLATUNJI O O, et al.Polysaccharides purified from Cordyceps cicadae protects PC12 cells against glutamate-induced oxidative damage[J].Carbohydrate Polymers, 2016, 153:187-195.
[27]
YAN J K, WANG Y Y, WANG Z B, et al.Structure and antioxidative property of a polysaccharide from an ammonium oxalate extract of Phellinus linteus[J].International Journal of Biological Macromolecules, 2016, 91:92-99.
[28]
SHEN S, CHENG H R, LI X, et al.Effects of extraction methods on antioxidant activities of polysaccharides from Camellia seed cake[J].European Food Research and Technology, 2014, 238(6):1 015-1 021.
[29]
巫永华, 陆文静, 刘梦虎, 等.响应面优化超声波辅助双水相提取牛蒡多糖及抗氧化分析[J].食品与发酵工业, 2020, 46(5):215-223.WU Y H, LU W J, LIU M H, et al.Optimization of ultrasonic-assisted aqueous two-phase extraction of burdock polysaccharide by response surface design and its antioxidant activities[J].Food and Fermentation Insustries, 2020, 46(5):215-223.
[30]
SHI J M, CHENG C L, ZHAO H T, et al.In vivo anti-radiation activities of the Ulva pertusa polysaccharides and polysaccharide-iron(III) complex[J].International Journal of Biological Macromolecules, 2013, 60:341-346.
[31]
YAN Y S, JI Y, SU N, et al.Non-anticoagulant effects of low molecular weight heparins in inflammatory disorders:A review[J].Carbohydrate Polymers, 2017, 160:71-81.
[32]
ZHANG X R, QI C H, GUO Y, et al.Toll-like receptor 4-related immunostimulatory polysaccharides:Primary structure, activity relationships, and possible interaction models[J].Carbohydrate Polymers, 2016, 149:186-206.
[33]
王全, 李红亚, 李术娜, 等.Box-Behnken设计响应面法优化超声波辅助双水相法提取玛咖多糖[J].河北大学学报(自然科学版), 2016, 36(4):387-395.WANG Q, LI H Y, LI S N, et al.Box-Behnken design for optimized extraction of polysaccharides from maca via ultrasonic-assisted aqueous two-phase method[J].Journal of Hebei University (Natural Science Edition), 2016, 36(4):387-395.
[34]
孙诗清, 王玉洁, 朱长俊, 等.超声波辅助双水相提取虫草多糖的工艺研究[J].安徽农业科学, 2012, 40(33):16 392-16 394;16 500.SUN S Q, WANG Y J, ZHU C J, et al.Extraction conditions of polysaccharide from cultured Cordyceps militaris by ultrasonic assisted aqueous two phase system[J].Journal of Anhui Agricultural Sciences, 2012, 40(33):16 392-16 394;16 500.
[35]
凡军民, 谢正林, 谢春芹, 等.超声波辅助双水相体系提取蝉花多糖及抗氧化研究[J].食品科技, 2020, 45(5):196-201.FAN J M, XIE Z L, XIE C Q, et al.Optimization of ultrasonic-assisted aqueous two-phase extraction and antioxidant activity of polysaccharide from Cordyceps cicadae[J].Food Science and Technology, 2020, 45(5):196-201.
2021, Vol. 47 
