Chemical composition of volatile oil from Citri retriculatae pericarpium and its antioxidant activity analysis
YU Xiangying, CHEN Xiaochun, LI Yuting, LI Lin*
(Engineering Research Center of Health Food Design & Nutrition Regulation,School of Chemical Engineering and Energy Technology,Dongguan University of Technology,Dongguan 523808,China)
Abstract: To explore the potential antioxidants contribution to the antioxidant activity of Citri retriculatae pericarpium volatile oil, the volatile oil was extracted from Xinhui “Chachi” and Fujian “Ponkan” by steam distillation and then was analyzed by GC-MS. In addition, DPPH/ABTS radical scavenging activity and ferric reducing/antioxidant activity were used to evaluate the antioxidant activity of volatile oil and the compounds in volatile oil. The results showed that a total of 23 compounds were identified in Citri retriculatae pericarpium volatile oil from both Xinhui and Fujian, with D-limonene of the highest content and γ-terpinene of the second-highest content. Citri retriculatae pericarpium volatile oil from Xinhui had a lower ratio of D-limonene/γ-terpinene and sesquiterpene. Additionally, methyl o-methylaminobenzoate and carveol could be detected only in Citri retriculatae pericarpium volatile oil from Xinhui. Furthermore, the antioxidant activity test showed that, compared with Citri retriculatae pericarpium volatile oil from Fujian, Citri retriculatae pericarpium volatile oil from Xinhui possesses the same radical scavenging activity and stronger reducing power. Among all tested compounds, carveol possesses the highest antioxidant activity which slightly smaller than Vc, followed by monoterpene (γ-terpinene, terpinolene, α-terpinene). D-limonene, aldehydes and alcohols have a lower antioxidant activity among all tested compounds. However, methyl ortho-methylaminobenzoate had no significant DPPH radical scavenging activity even though it had relatively strong ABTS scavenging activity and reducing ability. Through the composition analysis and antioxidant activity test, carveol, monoterpene (γ-terpinene, terpinolene, α-terpinene) and methyl ortho-methylaminobenzoate could be considered to be the main contributors to the antioxidant activity of volatile oil from Citri retriculatae pericarpium.
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2021, Vol. 47 
