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食品与发酵工业  2021, Vol. 47 Issue (9): 16-24    DOI: 10.13995/j.cnki.11-1802/ts.025886
  研究报告 本期目录 | 过刊浏览 | 高级检索 |
三种小麦麸皮总黄酮的体外抗氧化活性
冯艳钰, 臧延青*
(黑龙江八一农垦大学 食品学院,黑龙江 大庆,163000)
Study on the antioxidant activity of total flavonoids from three wheat brans in vitro
FENG Yanyu, ZANG Yanqing*
(Food Science College,Heilongjiang Bayi Agricultural University,Daqing 163000,China)
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摘要 研究不同溶剂(乙醇、甲醇、丙酮)提取的3种不同品种小麦麸皮(豫教5号、郑麦7698、西农979)黄酮类化合物的抗氧化活性。结果表明,3种不同的有机溶剂对小麦麸皮的总黄酮提取能力顺序为:乙醇>甲醇>丙酮。其中乙醇提取的总黄酮的总还原力和清除羟基自由基的能力最强。细胞实验结果显示,乙醇提取3种不同品种的小麦麸皮中黄酮类化合物能使人胃腺癌细胞(adenocarcinoma cell line,AGS)内活性氧水平下降,显示良好的抗氧化活性,且活性与总黄酮含量呈正相关性。总的来说,乙醇提取的西农979小麦麸皮的总黄酮的抗氧化能力最好。该研究结果为更好利用979小麦麸皮中黄酮类物质提供了参考依据。
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冯艳钰
臧延青
关键词:  小麦麸皮  总黄酮  抗氧化活性  1,1-二苯基-2-三硝基苯肼(1,1-diphenyl-2-picrylhydrazyl,DPPH)  总还原力  活性氧    
Abstract: In this study, the total flavonoids of three different varieties of wheat bran (Yujiao 5, Zhengmai 7698, Xinong 979) were extracted by different solvents (ethanol, methanol, acetone), and the effect of these flavonoids on the antioxidant activity was investigated. The results showed that the extraction ability of total flavonoids from wheat bran by different solvents was as follows ethanol > methanol > acetone. Among which the total flavonoids extracted by methanol had a greater scavenging ability on 1,1-diphenyl-2-picrylhydrazyl(DPPH) free radicals than those extracted by ethanol, whereas the total flavonoids extracted by ethanol displayed the strongest total reducing power and the ability to scavenge hydroxyl radicals. The result showed that three wheat bran acetone extracts decreased the level of reactive oxygen species (ROS) on human gastric adenocarcinoma cell line (AGS). The flavonoid compounds in three different varieties of wheat bran all showed good antioxidant activity in vitro, and the activity was positively correlated with the concentration of total flavonoids extracted by different solvents. In general, the total flavonoids of Xinong 979 wheat bran extracted by ethanol had the best antioxidant capacity. The research provide a references for better utilization of flavonoids in wheat bran.
Key words:  wheat bran    total flavonoids    antioxidant activity    DPPH    the total reducing power    reactive oxygen species
收稿日期:  2020-10-14      修回日期:  2020-12-01           出版日期:  2021-05-15      发布日期:  2021-06-03      期的出版日期:  2021-05-15
基金资助: 国家重点研发计划项目(SQ2018YFE020070)
作者简介:  硕士研究生(臧延青副教授为通讯作者,E-mail:byndzangyanqing@163.com)
引用本文:    
冯艳钰,臧延青. 三种小麦麸皮总黄酮的体外抗氧化活性[J]. 食品与发酵工业, 2021, 47(9): 16-24.
FENG Yanyu,ZANG Yanqing. Study on the antioxidant activity of total flavonoids from three wheat brans in vitro[J]. Food and Fermentation Industries, 2021, 47(9): 16-24.
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http://sf1970.cnif.cn/CN/10.13995/j.cnki.11-1802/ts.025886  或          http://sf1970.cnif.cn/CN/Y2021/V47/I9/16
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