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食品与发酵工业  2021, Vol. 47 Issue (10): 185-191    DOI: 10.13995/j.cnki.11-1802/ts.025890
  生产与科研应用 本期目录 | 过刊浏览 | 高级检索 |
球毛壳菌CGMCC 6882利用小麦秸秆发酵生产抗氧化多糖
王子朝1*, 郭佳源2, 郜文硕1, 崔静雯1, 杨青青1, 朱金帆1, 张慧茹1
1(河南工业大学 生物工程学院,河南 郑州,450001)
2(河南工业大学 国际教育学院,河南 郑州,450001)
Production of an antioxidant polysaccharide from wheat straw by Chaetomium globosum CGMCC 6882
WANG Zichao1*, GUO Jiayuan2, GAO Wenshuo1, CUI Jingwen1, YANG Qingqing1, ZHU Jinfan1, ZHANG Huiru1
1(College of Biological Engineering, Henan University of Technology, Zhengzhou 450001, China)
2(School of International Education, Henan University of Technology, Zhengzhou 450001, China)
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摘要 针对我国小麦秸秆存在严重浪费和污染环境的现状,该实验采用球毛壳菌CGMCC 6882对小麦秸秆进行发酵,生产一种新型胞外多糖,并对多糖结构和抗氧化活性进行研究。结果显示,球毛壳菌CGMCC 6882以小麦秸秆为碳源发酵所得胞外多糖的单糖组成和摩尔比为鼠李糖∶氨基葡萄糖∶半乳糖∶葡萄糖∶木糖∶果糖∶葡萄糖醛酸=21.46∶1.58∶1.11∶55.15∶36.37∶7.04∶7.34,重均分子质量Mw为3.538×104 Da。体外抗氧化实验结果显示,球毛壳菌CGMCC 6882胞外多糖可以有效清除DPPH自由基、羟自由基、超氧阴离子和ABTS阳离子自由基,且清除率与多糖浓度成正相关。多糖质量浓度为5 mg/mL时,对上述自由基的清除率分别达到(83.08±2.58)%、(81.39±1.47)%、(79.38±2.03)%和(85.69±1.62)%。细胞抗氧化实验结果显示,加入多糖实验组的丙二醛酶活水平从(15.8±0.25) μmol/g降低至(7.5±0.31) μmol/g,与多糖浓度成负相关;Caco-2细胞内超氧化物岐化酶、过氧化氢酶和谷胱甘肽过氧化物酶活力水平分别从(147.28±4.82)、(11±1.15)和(18.08±1.96) U/mg增加至(238.89±3.91)、(39.12±0.76)和(41.35±2.18) U/mg,且与多糖浓度成正相关。该实验为小麦秸秆的高效利用提供了重要依据和理论基础。
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王子朝
郭佳源
郜文硕
崔静雯
杨青青
朱金帆
张慧茹
关键词:  小麦秸秆  球毛壳菌CGMCC 6882  多糖  结构特征  抗氧化活性    
Abstract: Due to the serious resource waste and environmental pollution induced by wheat straw in China, wheat straw was used by Chaetomium globosum CGMCC 6882 to produce exopolysaccharide in present work. Meanwhile, the structure of the exopolysaccharide and its antioxidant activity were investigated. Results showed that monosaccharide composition of the polysaccharide produced by C. globosum CGMCC 6882 was rhamnose, glucosamine, galactose, glucose, xylose, fructose and glucuronic acid in a molar ratio of 21.46∶1.58∶1.11∶55.15∶36.37∶7.04∶7.34, and its molecular weight was 3.538×104 Da. Antioxidant assays in vitro revealed that the polysaccharide had strong scavenging effects on DPPH radical, hydroxyl radical, superoxide anions and ABTS radical in a dose-dependent manner. At the concentration of 5 mg/mL, the corresponding scavenging abilities of the polysaccharide were (83.08±2.58)%, (81.39±1.47)%, (79.38±2.03)% and (85.69±1.62)%, respectively. Results of cell antioxidant test showed that the activity of MDA decreased from (15.8±0.25) μmol/g to (7.5±0.31) μmol/g, which was negatively correlated with the concentration of polysaccharide. Furthermore, the activity levels of SOD, CAT and GSH-Px in H2O2 induced Caco-2 cells increased from (147.28±4.82), (11±1.15) and (18.08±1.96) U/mg to (238.89±3.91), (39.12±0.76) and (41.35±2.18) U/mg, and those were positively correlated with the concentration of polysaccharide. Overall, this work provides an important reference and theoretical basis for the efficient utilization of wheat straw.
Key words:  wheat straw    Chaetomium globosum CGMCC 6882    polysaccharide    structural feature    antioxidant activity
收稿日期:  2020-10-14      修回日期:  2020-11-24           出版日期:  2021-05-25      发布日期:  2021-06-17      期的出版日期:  2021-05-25
基金资助: 河南省高等学校重点科研计划(19A180015);河南省谷物资源转化与利用重点实验室开放课题(PL2018006);河南工业大学高层次人才基金项目(2017BS011)
作者简介:  博士,讲师(本文通讯作者,E-mail:6832917@163.com)
引用本文:    
王子朝,郭佳源,郜文硕,等. 球毛壳菌CGMCC 6882利用小麦秸秆发酵生产抗氧化多糖[J]. 食品与发酵工业, 2021, 47(10): 185-191.
WANG Zichao,GUO Jiayuan,GAO Wenshuo,et al. Production of an antioxidant polysaccharide from wheat straw by Chaetomium globosum CGMCC 6882[J]. Food and Fermentation Industries, 2021, 47(10): 185-191.
链接本文:  
http://sf1970.cnif.cn/CN/10.13995/j.cnki.11-1802/ts.025890  或          http://sf1970.cnif.cn/CN/Y2021/V47/I10/185
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