Abstract: Zearalenone and its derivatives are the most widely-existed mycotoxins around the world, causing huge safety hazards and economic losses to humans.Traditional physical and chemical detoxification methods cannot meet the demands of industrial applications due to their incomplete detoxification, destruction to food texture, and loss of nutrients.In recent years, biological and enzymatic degradation of mycotoxins has gradually been studied and reported due to its wide range of microbial sources, mild enzyme reaction conditions and high detoxification efficiency.Particularly, the lactone has been extensively studied due to its high ZEN degradation activity, clear detoxification mechanism, irreversible detoxification process and elucidated crystal structure.This article reviewed the research progress of lactone in terms of gene mining, characterization, molecular modification and application exploration.The purpose is to clarify the advantages and challenges in the industrial application of the zearalenone-detoxifying lactone, and provide certain references for its future research on zearalenone biodegradation.
SHI H T,LI S L,Bai Y Y,et al.A Mycotoxin contamination of food and feed in China occurrence,detection techniques,toxicological effects and advances in mitigation technologies[J].Food Control,2018,91:202-215.
[2]
LOI M,FANELLI F,LIUZZI V C,et al.Mycotoxin biotransformation by native and commercial enzymes:Present and future perspectives[J].Toxins (Basel),2017,9(4):111-142.
[3]
FRUHAUF S,NOVAK B,NAGL V,et al.Biotransformation of the mycotoxin zearalenone to its metabolites hydrolyzed zearalenone (hzen) and decarboxylated hydrolyzed zearalenone (dhzen) diminishes its estrogenicity in vitro and in vivo[J].Toxins,2019,11(8).DOI:10.3390/toxins11080481.
[4]
SZABÓ-FODOR J,SZABÓ A,KÓCSÓ D,et al.Interaction between the three frequently co-occurring Fusarium mycotoxins in rats[J].Journal of Animal Physiology and Animal Nutrition,2019,103(1):370-382.
[5]
ROGOWSKA A,POMASTOWSKI P,SAGANDYKOVA G,et al.Zearalenone and its metabolites:Effect on human health,metabolism and neutralisation methods[J].Toxicon,2019,162:46-56.
[6]
GONZALEZ PEREYRA M L,DI GIACOMO AL,LARA A L,et al.Aflatoxin-degrading Bacillus sp.strains degrade zearalenone and produce proteases,amylases and cellulases of agro-industrial interest[J].Toxicon,2020,180:43-48.
[7]
KALAGATUR N K,KAMASANI J R,MUDILI V.Assessment of detoxification efficacy of irradiation on zearalenone mycotoxin in various fruit juices by response surface methodology and elucidation of its in-vitro toxicity[J].Frontiers in Microbiology,2018,9:2 937-2 950.
[8]
BAI X,SUN C,XU J,et al.Detoxification of zearalenone from corn oil by adsorption of functionalized GO systems[J].Applied Surface Science,2018,430:198-207.
[9]
VILA-DONAT P,MARíN S,SANCHIS V,et al.A review of the mycotoxin adsorbing agents,with an emphasis on their multi-binding capacity,for animal feed decontamination[J].Food and Chemical Toxicology,2018,114:246-259.
[10]
XU Y,WANG Y,JI J,et al.Chemical and toxicological alterations of zearalenone under ozone treatment[J].Food Additives & Contaminants:Part A,2019,36(1):163-174.
[11]
TANG Y,XIAO J,CHEN Y,et al.Secretory expression and characterization of a novel peroxiredoxin for zearalenone detoxification in Saccharomyces cerevisiae[J].Microbiological Research,2013,168(1):6-11.
[12]
YU Y,QIU L,WU H,et al.Oxidation of zearalenone by extracellular enzymes from Acinetobacter sp.SM04 into smaller estrogenic products[J].World Journal of Microbiology and Biotechnology,2011,27(11):2 675-2 681.
[13]
YU Y,WU H,TANG Y,et al.Cloning,expression of a peroxiredoxin gene from Acinetobacter sp.SM04 and characterization of its recombinant protein for zearalenone detoxification[J].Microbiological Research,2012,167(3):121-126.
[14]
LOI M,FANELLI F,CIMMARUSTI M T,et al.In vitro single and combined mycotoxins degradation by Ery4 laccase from Pleurotus eryngii and redox mediators[J].Food Control,2018,90:401-406.
[15]
卢丹, 徐晴,江凌,等.生物酶降解真菌毒素的研究进展[J].生物加工过程,2018,16(2):49-56.LU D,XU Q,JIANG L,et al.Advances in enzymatic detoxification of mycotoxins[J].The Chinese Journal of Bioprocessing Engineering,2018,16(2):49-56
[16]
TAKAHASHI-ANDO N,KIMURA M,KAKEYA H,et al.A novel lactonohydrolase responsible for the detoxification of zearalenone:Enzyme purification and gene cloning[J].Biochemical Journal,2002,365:1-6.
[17]
YU X,TU T,LUO H,et al.Biochemical characterization and mutational analysis of a lactone hydrolase from Phialophora americana[J].Journal of Agricultural and Food Chemistry,2020,68(8):2 570-2 577.
[18]
TAKAHASHI-ANDO N,OHSATO S,SHIBATA T,et al.Metabolism of zearalenone by genetically modified organisms expressing the detoxification gene from Clonostachys rosea[J].Applied Environmental and Microbiology,2004,70(6):3 239-3 345.
[19]
YANG W C,HSU T C,CHENG K C,et al.Expression of the Clonostachys rosea lactonohydrolase gene by Lactobacillus reuteri to increase its zearalenone-removing ability[J].Microbial Cell Factories,2017,16(1):1-11.
[20]
BI K,ZHANG W,XIAO Z,et al.Characterization,expression and application of a zearalenone degrading enzyme from Neurospora crassa[J].AMB Express,2018,8(1):1-10.
[21]
WANG M,YIN L,HU H,et al.Expression,functional analysis and mutation of a novel neutral zearalenone-degrading enzyme[J].International Journal of Biological Macromolecules,2018,118:1 284-1 292.
[22]
HUI R,HU X,LIU W,et al.Characterization and crystal structure of a novel zearalenone hydrolase from Cladophialophora bantiana[J].Acta Cryst F Structural Biological Communications,2017,73(Pt 9):515-519.
[23]
XIANG L,WANG Q,ZHOU Y,et al.High-level expression of a ZEN-detoxifying gene by codon optimization and biobrick in Pichia pastoris[J].Microbiological Research,2016,193:48-56.
[24]
胡翔颖, 刘文婷,刘卫东,等.来源于Rhinocladiella mackenziei的玉米赤霉烯酮水解酶的表达纯化与酶学性质[J].微生物学通报,2018,45(12):2 585-2 591.HU X Y,LIU W T,LIU W D,et al.Expression,purification and characterization of a novel zearalenone hydrolase from Rhinocladiella mackenziei[J].Microbiology China,2018,45(12):2 585-2 591.
[25]
刘海燕, 孙长坡,伍松陵,等.玉米赤霉烯酮毒素降解酶基因的克隆及在毕赤酵母中的高效表达[J].中国粮油学报,2011,26:12-17.LIU H Y,SUN C P,WU S L,et al.Expression of zearalenone degrading enzyme gene from Gliocladium roseum in Pichia pastoris GS115[J].Journal of the Chinese Cereals and Oils Association,2011,26:12-17.
[26]
程波财, 史文婷,罗洁,等.玉米赤霉烯酮降解酶基因(ZEN-jjm)的克隆、表达及活性分析[J].农业生物技术学报,2010,18(2):225-230.CHENG B C,SHI W T,LUO H,et al.Cloning of zearalenone-degraded enzyme gene (ZEN-jjm) and its expression and activity analysis[J].Journal Of Agricultural Biotechnology,2010,18(2):225-230.
[27]
ZHANG Z X,XU W,WU H,et al.Identification of a potent enzyme for the detoxification of zearalenone[J].Journal of Agricultural and Food Chemistry,2020,68(1):376-383.
[28]
LIU Q,XUN G,FENG Y.The state-of-the-art strategies of protein engineering for enzyme stabilization[J].Biotechnology Advances,2019,37(4):530-537.
[29]
XU Z,LIU W,CHEN C C,et al.Enhanced α-zearalenol hydrolyzing activity of a mycoestrogen-detoxifying lactonase by structure-based engineering[J].ACS Catalysis,2016,6(11):7 657-7 663.
[30]
ZHENG Y,LIU W,CHEN C C,et al.Crystal structure of a mycoestrogen-detoxifying lactonase from Rhinocladiella mackenziei:Molecular insight into ZHD substrate selectivity[J].ACS Catalysis,2018,8(5):4 294-4 298.
[31]
PENG W,KO T P,YANG Y,et al.Crystal structure and substrate-binding mode of the mycoestrogen-detoxifying lactonase ZHD from Clonostachys rosea[J].RSC Advances,2014,4(107):62 321-62 325.
[32]
许中霞, 刘桂智,刘卫东,等.玉米赤霉烯酮水解酶耐热性的分子改造[J].食品与生物技术学报,2019,38(7):71-77.XU Z X,LIU G Z,LIU W D,et al.Molecular engineering of mycoestrogen-detoxifying lactonase ZHD101 to improve enzyme thermostability[J].Journal of Food Science and Biotechnology,2019,38(7):71-77.
[33]
王壬丰, 周峻岗,胡小健,等.玉米赤霉烯酮降解酶在马克斯克鲁维酵母中的 表达及高产菌株的诱变筛选[J].复旦学报(自然科学版),2017,56(4):431-437.WANG R F,ZHOU J G,HU X J,et al.Expression of ZHD1O1 in Kluyveromyces marxianus and the mutation breeding of high-field strain[J].Journal of Fudan University(Natural Science),2017,56(4):431-437.
[34]
HE M,LI Y,PI F,et al.A novel detoxifying agent:Using rice husk carriers to immobilize zearalenone-degrading enzyme from Aspergillus niger FS10[J].Food Control,2016,68:271-279.
[35]
王浩宇, 孙晶,常晓娇,等.玉米赤霉烯酮降解酶ZLHY6的包埋及稳定性研究[J].粮油食品科技,2018,26(2):63-67.WANG H Y,SUN J,CHANG X J,et al.Study on the embedding and stability of zearalenone degrading enzyme ZLHY6[J].Science and Technology of Cereals,Oils and Foods,2018,26(2):63-67.
[36]
FILEK M,SIEPRAWSKA A,KOS′CIELNIAK J,et al.The role of chloroplasts in the oxidative stress that is induced by zearalenone in wheat plants-The functions of 24-epibrassinolide and selenium in the protective mechanisms[J].Plant Physiology and Biochemistry,2019,137:84-92.
[37]
FAISAL Z,FLISZÁR-NYÙL E,DELLAFIORA L,et al.Interaction of zearalenone-14-sulfate with cyclodextrins and the removal of the modified mycotoxin from aqueous solution by beta-cyclodextrin bead polymer[J].Journal of Molecular Liquids,2020,310.DOI:10.1016/j.molliq.2020.113236.
[38]
HIGA-NISHIYAMA A,TAKAHASHI-ANDO N,SHIMIZU T,et al.A model transgenic cereal plant with detoxification activity for the estrogenic mycotoxin zearalenone[J].Transgenic Research,2005,14(5):713-717.
[39]
IGAWA T,TAKAHASHI-Ando N,OCHIAi N,et al.Reduced contamination by the Fusarium mycotoxin zearalenone in maize kernels through genetic modification with a detoxification gene[J].Applied and Environmenal Microbiology,2007,73(5):1 622-1 629.
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