Abstract: Bacterial biofilm model of periodontitis and cell model of periodontitis were applied to screen Lactobacilli strains for treating periodontitis. Lactobacilli were evaluated by inhibiting the amount of biofilm formation, reducing the ability of inflammatory factors IL-1β, IL-8, TNF-α, and regulating the expression levels of Claudin-1 and Occludin. Lactobacillus plantarum CCFM1137 and Lactobacillus fermentum CCFM1139 were screened, which could inhibit the formation of mixed bacterial biofilm, reduce oral inflammation, and regulate the expression of tight junction protein. Then the oral probiotic properties of the two Lactobacilli strains were evaluated by measuring the lysozyme tolerance ability, self-forming ability, self-polymerization ability and co-polymerization ability. The results showed that L. plantarum CCFM1137 and L. fermentum CCFM1139 have application potentials as oral probiotics for treating periodontitis.
CHAPPLE I L C,VAN DER WEIJDEN F,DOERFER C,et al.Primary prevention of periodontitis:Managing gingivitis[J].Journal of Clinical Periodontology,2015,42:S71-S76.
[2]
YU Y H,CHASMAN D I,BURING J E,et al.Cardiovascular risks associated with incident and prevalent periodontal disease[J].Journal of Clinical Periodontology,2015,42(1):21-28.
[3]
CHEN S J,SU T T,ZHANG Y,et al.Fusobacterium nucleatum promotes colorectal cancer metastasis by modulating KRT7-AS/KRT7[J].Gut Microbes,2020,11(3):511-525.
[4]
孟焕新. 牙周病学[M].第三版.北京:人民卫生出版社,2011.MENG H X.Periodontology[M].3rd ed.Beijing:People’s Medical Publishing House,2011.
[5]
TZACH-NAHMAN R,MIZRAJI G,SHAPIRA L,et al.Oral infection with Porphyromonas gingivalis induces peri-implantitis in a murine model:Evaluation of bone loss and the local inflammatory response[J].Journal of Clinical Periodontology,2017,44(7):739-748.
[6]
SUN J,NEMOTO E,HONG G,et al.Modulation of stromal cell-derived factor 1 alpha(SDF-1α) and its receptor CXCR4 in Porphyromonas gingivalis-induced periodontal inflammation[J].BMC Oral Health,2016,17(1):26.
[7]
谢晓婷, 王以玲,肖水清.具核梭杆菌的致病性与其可引起的口腔疾病[J].中国医学创新,2017,14(10):145-148.XIE X T,WANG Y L,XIAO S Q.Pathogenicity and oral disease caused by Fusobacterium nucleatum[J].Medical Innovation of China,2017,14(10):145-148.
[8]
KOLENBRANDER P E.Multispecies communities:Interspecies interactions influence growth on saliva as sole nutritional source[J].International Journal of Oral Science,2011,3(2):49-54.
[9]
HUCK O,AL-HASHEMI J,POIDEVIN L,et al.Identification and characterization of MicroRNA differentially expressed in macrophages exposed to Porphyromonas gingivalis infection[J].Infection and Immunity,2017,85(3):771.
[10]
MATTHEWS D C,ROCCHI A,GAFNI A.Factors affecting patients’ and potential patients’ choices among anaesthetics for periodontal recall visits[J].Journal of Dentistry,2001,29(3):173-179.
[11]
MARSH P D.Dental plaque as a biofilm and a microbial community-implications for health and disease[J].BMC Oral Health,2006,6(Suppl 1):S14.
[12]
YU R,ZUO F L,MA H Q,et al.Exopolysaccharide-producing Bifidobacterium adolescentis strains with similar adhesion property induce differential regulation of inflammatory immune response in treg/Th17 axis of DSS-colitis mice[J].Nutrients,2019,11(4):E782.
[13]
WANNUN P,PIWAT S,TEANPAISAN R.Purification and characterization of bacteriocin produced by oral Lactobacillus paracasei SD1[J].Anaerobe,2014,27:17-21.
[14]
TERAI T,OKUMURA T,IMAI S,et al.Screening of probiotic candidates in human oral bacteria for the prevention of dental disease[J].PLoS One,2015,10(6).DOI:10.1371/journal.pone.0128657.
[15]
GERALDO B M C,BATALHA M N,MILHAN N V M,et al.Heat-killed Lactobacillus reuteri and cell-free culture supernatant have similar effects to viable probiotics during interaction with Porphyromonas gingivalis[J].Journal of Periodontal Research,2020,55(2):215-220.
[16]
KANG M S,OH J S,LEE H C,et al.Inhibitory effect of Lactobacillus reuteri on periodontopathic and cariogenic bacteria[J].The Journal of Microbiology,2011,49(2):193-199.
[17]
WANG L Y,LUO Q M,ZHANG X M,et al.Co-implantation of magnesium and zinc ions into titanium regulates the behaviors of human gingival fibroblasts[J].Bioactive Materials,2021,6(1):64-74.
[18]
张秋香, 黄银,姚沛琳,等.植物乳杆菌FB-T9抑制变异链球菌及其生物膜形成的研究[J].食品与生物技术学报,2019,38(9):17-26.ZHANG Q X,HUANG Y,YAO P L,et al.Inhibition of Streptococcus mutans and its biofilm formation by Lactobacillus plantarum FB-T9[J].Journal of Food Science and Biotechnology,2019,38(9):17-26.
[19]
HASAN S,DANISHUDDIN M,KHAN A U.Inhibitory effect of Zingiber officinale towards Streptococcus mutans virulence and caries development:In vitro and in vivo studies[J].BMC Microbiology,2015,15(1):1-14.
[20]
KOS B,S^US^KOVIC′ J,VUKOVIC′ S,et al.Adhesion and aggregation ability of probiotic strain Lactobacillus acidophilus M92[J].Journal of Applied Microbiology,2003,94(6):981-987.
[21]
POLAK-BERECKA M,WAC′KO A,PADUCH R,et al.The effect of cell surface components on adhesion ability of Lactobacillus rhamnosus[J].Antonie Van Leeuwenhoek,2014,106(4):751-762.
[22]
GUO W,WANG P,LIU Z H,et al.Analysis of differential expression of tight junction proteins in cultured oral epithelial cells altered by Porphyromonas gingivalis,Porphyromonas gingivalis lipopolysaccharide,and extracellular adenosine triphosphate[J].International Journal of Oral Science,2018,10(1).DOI:10.1038/ijos.2017.51.
[23]
于亚男, 吴正钧,韩瑨.唾液链球菌BD3900作为口腔益生菌的特性[J].食品与机械,2017,33(11):13-17.YU Y N,WU Z J,HAN J.Characteristics of Streptococcus salivarius BD3900 as an oral probiotics[J].Food & Machinery,2017,33(11):13-17.
[24]
秦苏佳, 徐晚晴,张秋香,等.植物乳杆菌CCFM8724对致龋双菌生物膜的抑制作用[J].食品与发酵工业,2020,46(13):127-132.QIN S J,XU W Q,ZHANG Q X,et al.Inhibitory effect of Lactobacillus plantarum CCFM8724 on dual caries-causing dual biofilms[J].Food and Fermentation Industries,2020,46(13):127-132.
[25]
陆文伟, 林炳谕,尹一婷,等.双歧杆菌生物膜成膜规律及特性研究[J].中国食品学报,2017,17(12):42-49.LU W W,LIN B Y,YIN Y T,et al.The biofilm formation of Bifidobacterium and its characteristics[J].Journal of Chinese Institute of Food Science and Technology,2017,17(12):42-49.
[26]
ABBASILIASI S,TAN J S,BASHOKOUH F,et al.In vitro assessment of Pediococcus acidilactici Kp10 for its potential use in the food industry[J].BMC Microbiology,2017,17(1):121.
[27]
KELLER M K,HASSLOF P,STECKSÉN-BLICKS C,et al.Co-aggregation and growth inhibition of probiotic lactobacilli and clinical isolates of mutans streptococci:An in vitro study[J].Acta Odontologica Scandinavica,2011,69(5):263-268.