Abstract: Bacterial biofilm model of periodontitis and cell model of periodontitis were applied to screen Lactobacilli strains for treating periodontitis. Lactobacilli were evaluated by inhibiting the amount of biofilm formation, reducing the ability of inflammatory factors IL-1β, IL-8, TNF-α, and regulating the expression levels of Claudin-1 and Occludin. Lactobacillus plantarum CCFM1137 and Lactobacillus fermentum CCFM1139 were screened, which could inhibit the formation of mixed bacterial biofilm, reduce oral inflammation, and regulate the expression of tight junction protein. Then the oral probiotic properties of the two Lactobacilli strains were evaluated by measuring the lysozyme tolerance ability, self-forming ability, self-polymerization ability and co-polymerization ability. The results showed that L. plantarum CCFM1137 and L. fermentum CCFM1139 have application potentials as oral probiotics for treating periodontitis.
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2021, Vol. 47 
