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食品与发酵工业  2021, Vol. 47 Issue (15): 70-76    DOI: 10.13995/j.cnki.11-1802/ts.025948
  研究报告 本期目录 | 过刊浏览 | 高级检索 |
治疗牙周炎的乳杆菌筛选及口腔益生特性评价
徐晚晴1, 张秋香1*, 郑彦懿2, 赵建新1, 马方励2*
1(江南大学 食品学院,江苏 无锡,214122)
2(无限极(中国)有限公司,广东 江门,519000)
Screeningand evaluation of Lactobacillus for treatment of periodontitis
XU Wanqing1, ZHANG Qiuxiang1*, ZHENG Yanyi2, ZHAO Jianxin1, MA Fangli2*
1(School of Food Science and Technology,Jiangnan University,Wuxi 214122,China)
2(Infinitus (China) Co.Ltd,Jiangmen 519000,China)
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摘要 利用牙周炎致病菌混合生物膜模型和牙周炎细胞模型筛选具有缓解牙周炎作用的乳杆菌。通过抑制生物膜形成量、降低炎性因子白细胞介素-1β、白细胞介素-8、肿瘤坏死因子-α能力、调节紧密连接蛋白Claudin-1、Occludin基因表达水平对乳杆菌进行评估。筛选得到2株具有抑制混菌生物膜形成、缓解口腔炎症、调控紧密连接蛋白表达的植物乳杆菌CCFM1137和发酵乳杆菌CCFM1139。而后通过测定溶菌酶耐受能力、自成膜能力、自聚和共聚能力对2株乳杆菌进行口腔益生特性评价。结果表明,植物乳杆菌CCFM1137和发酵乳杆菌CCFM1139有作为治疗牙周炎的口腔益生菌的应用潜力。
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徐晚晴
张秋香
郑彦懿
赵建新
马方励
关键词:  牙周炎  生物膜  炎性因子  紧密连接蛋白  乳杆菌    
Abstract: Bacterial biofilm model of periodontitis and cell model of periodontitis were applied to screen Lactobacilli strains for treating periodontitis. Lactobacilli were evaluated by inhibiting the amount of biofilm formation, reducing the ability of inflammatory factors IL-1β, IL-8, TNF-α, and regulating the expression levels of Claudin-1 and Occludin. Lactobacillus plantarum CCFM1137 and Lactobacillus fermentum CCFM1139 were screened, which could inhibit the formation of mixed bacterial biofilm, reduce oral inflammation, and regulate the expression of tight junction protein. Then the oral probiotic properties of the two Lactobacilli strains were evaluated by measuring the lysozyme tolerance ability, self-forming ability, self-polymerization ability and co-polymerization ability. The results showed that L. plantarum CCFM1137 and L. fermentum CCFM1139 have application potentials as oral probiotics for treating periodontitis.
Key words:  periodontitis    biofilm    inflammatory factor    tight junction protein    Lactobacilli
收稿日期:  2020-10-20      修回日期:  2020-12-04           出版日期:  2021-08-15      发布日期:  2021-08-23      期的出版日期:  2021-08-15
基金资助: 国家自然科学基金项目(32072197)
作者简介:  硕士研究生(张秋香副教授与马方励工程师为共同通讯作者,E-mail:zhangqx@jiangnan.edu.cn;mary.ma@infinitus-int.com)
引用本文:    
徐晚晴,张秋香,郑彦懿,等. 治疗牙周炎的乳杆菌筛选及口腔益生特性评价[J]. 食品与发酵工业, 2021, 47(15): 70-76.
XU Wanqing,ZHANG Qiuxiang,ZHENG Yanyi,et al. Screeningand evaluation of Lactobacillus for treatment of periodontitis[J]. Food and Fermentation Industries, 2021, 47(15): 70-76.
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http://sf1970.cnif.cn/CN/10.13995/j.cnki.11-1802/ts.025948  或          http://sf1970.cnif.cn/CN/Y2021/V47/I15/70
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