1(College of Food Science and Technology,Guangdong Ocean University,Guangdong Provincial Key Laboratory of Aquatic Product Processing and Safety,Zhanjiang 524088,China) 2(Guangdong Provincial Modern Agricultural Science and Technology Innovation Center for Subtropical Fruit and Vegetable Processing,Zhanjiang 524088,China)
Abstract: To investigate the effect of Camellia nitidissima Chi flower on protein digestion and absorption, the effect of C. nitidissima Chi flower extract on the digestive protease activity and the inhibition kinetics of digestive protease were studied using fluorescence spectroscopy, ultraviolet spectroscopy, and circular dichroism. The results showed that both ethanol extract and aqueous extract of C. nitidissima Chi flower exhibited obvious inhibitory effect on pepsin (IC50 were 3.194 and 3.330 g/L; competition inhibition constant was 4.518 and 7.641 g/L, respectively) and trypsin activity (IC50 were 29.131 and 56.534 g/L; competition inhibition constant were 74.571 and 175.832 g/L, respectively), and the inhibition types were mixed manner. The spectrum experiment demonstrated that C. nitidissima Chi flower extract reduced the endogenous fluorescence of pepsin (quenching constant were 1.522 and 1.205 L/g, respectively) and trypsin (Quenching constant were 2.175 and 1.392 L/g, respectively), and further changed the secondary structure of digestive protease. Therefore, this study proved that the C. nitidissima Chi flower extracts could inhibit digestive protease activity by changing its structure, which provides theoretical support for the development of functional foods containing C. nitidissima Chi flower.
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