Abstract: To investigate the bacterial community and diversity of grapevine plants grown in southeastern Tibet, the bacterial community and diversity of grape and rhizosphere soil of different provenances in southeastern Tibet were analyzed by high-throughput sequencing. The results showed that a total of 366 720 valid sequences and 2 655 operational taxonomic units (OTUs) were obtained across all libraries, representing 713 genera in 32 bacterial phyla. There were similarities and differences in bacterial communities among different samples, and the rhizosphere soil showed the highest number and diversity of bacteria. The Proteobacteria and Actinobacteria were the predominant bacterial phyla in all samples, accounting for 21.31%-69.08% and 21.41%-44.60%, respectively. Burkholderia (27.60%) andnorank_f_Muribaculaceae (5.34%) were the predominant genera in Linzhi grape, while Gluconobacter (22.53%), Frigoribacterium (14.14%) and Pantoea (13.52%) were the predominant genera in Mangkang grape. Arthrobacter (11.49%)、unclassified _Enterobacteriaceae(7.43%) and Nocardioides (5.15%) were the predominant genera in Linzhi soil, while Gaiella (5.17%) and norank_f_Llumatobacteraceae(4.43%) were the predominant genera in Mangkang soil. The predominant bacteria were significantly different in different samples. Principal coordinates analysis (PCoA) showed that the bacterial community in grape samples was similar, however, the bacterial community of rhizosphere soil was significantly different. The study provides a theoretical basis for grape planting and utilization of distinctive microorganisms in the wine industry.
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