Abstract: To investigate the bacterial community and diversity of grapevine plants grown in southeastern Tibet, the bacterial community and diversity of grape and rhizosphere soil of different provenances in southeastern Tibet were analyzed by high-throughput sequencing. The results showed that a total of 366 720 valid sequences and 2 655 operational taxonomic units (OTUs) were obtained across all libraries, representing 713 genera in 32 bacterial phyla. There were similarities and differences in bacterial communities among different samples, and the rhizosphere soil showed the highest number and diversity of bacteria. The Proteobacteria and Actinobacteria were the predominant bacterial phyla in all samples, accounting for 21.31%-69.08% and 21.41%-44.60%, respectively. Burkholderia (27.60%) andnorank_f_Muribaculaceae (5.34%) were the predominant genera in Linzhi grape, while Gluconobacter (22.53%), Frigoribacterium (14.14%) and Pantoea (13.52%) were the predominant genera in Mangkang grape. Arthrobacter (11.49%)、unclassified _Enterobacteriaceae(7.43%) and Nocardioides (5.15%) were the predominant genera in Linzhi soil, while Gaiella (5.17%) and norank_f_Llumatobacteraceae(4.43%) were the predominant genera in Mangkang soil. The predominant bacteria were significantly different in different samples. Principal coordinates analysis (PCoA) showed that the bacterial community in grape samples was similar, however, the bacterial community of rhizosphere soil was significantly different. The study provides a theoretical basis for grape planting and utilization of distinctive microorganisms in the wine industry.
张世伟. 酿酒葡萄微生物群落多样性及其氮代谢通量研究[D].北京:中国矿业大学(北京), 2018.ZHANG S W.Study on microbial community diversity and the nitrogen metabolism flux of wine grapes[D].Beijing:China University of Mining & Technology (Beijing), 2018.
[2]
ALEKLETT K, LEFF J W, FIERER N, et al.Wild plant species growing closely connected in a subalpine meadow host distinct root-associated bacterial communities[J].Peer J, 2015, 3(8):e804.
[3]
CHAUDHRY V, REHMAN A, MISHRA A, et al.Changes in bacterial community structure of agricultural land due to long-term organic and chemical amendments[J].Microbial Ecology, 2012, 64(2):450-460.
[4]
RENOUF V, CLAISSE O, LONVAUD-FUNEL A.Numeration, identification and understanding of microbial biofilm on grape berry surface[J].Australian Journal of Grape and Wine Research, 2005, 11:316-327.
[5]
OCÓN E, GUTIÉRREZ A R, GARIJO P, et al.Presence of non-Saccharomyces yeasts in cellar equipment and grape juice during harvest time[J].Food Microbiology, 2010, 27(8):1 023-1 027.
[6]
MARTINS G, LAUGA B, MIOT-SERTIER C, et al.Characterization of epiphytic bacterial communities from grapes, leaves, bark and soil of grapevine plants grown, and their relations[J].PLoS One, 2013, 8(8):e73 013.
[7]
BOKULICH N A, THORNGATE J H, RICHARDSON P M, et al.Microbial biogeography of wine grapes is conditioned by cultivar, vintage, and climate[J].Proceedings of the National Academy of Sciences of the United States of America, 2014, 111(1):e139-e148.
[8]
魏玉洁, 邹弯, 马文瑞, 等.应用高通量测序技术研究新疆产区葡萄果实、叶片及果园土壤微生物多样性[J].食品科学, 2018, 39(6):162-170.WEI Y J, ZOU W, MA W R, et al.Microbial diversity of berries, leaves and soil of grapevine plants grown in Xinjiang analyzed by high-throughput sequencing[J].Food Science, 2018, 39(6):162-170.
[9]
RIBÉREAU-GAYON P, DUBOURDIEU D, DONÈCHE B.Handbook of Enology:The Microbiology of Wine and Vinifications[M].New York:John Wiley and Sons, 2006:18-37.
[10]
王伟, 布丽根·加冷别克, 胡晓东, 等.基于高通量测序技术的酿酒葡萄产区土壤微生物多样性[J].新疆农业科学, 2020, 57(5):859-868.WANG W, BULIGEN J L B K, HU X D, et al.Analysis of the microbial community diversity of soil from wine grape producting area in Xinjiang based on high-throughput sequencing[J].Xinjiang Agricultural Sciences, 2020, 57(5):859-868.
[11]
WU L Y, WEN C Q, QIN Y J, et al.Phasing amplicon sequencing on Illumina MiSeq for robust environmental microbial community analysis[J].BMC Microbiology, 2015, 15(1):125.
[12]
YOU J, WU G, REN F P, et al.Microbial community dynamics in Baolige oilfield during MEOR treatment, revealed by Illumina MiSeq sequencing[J].Applied Microbiology & Biotechnology, 2016, 100(3):1 469-1 478.
[13]
鲍士旦. 土壤农化分析[M].第三版.北京:中国农业出版社, 2000.BAO S D.Soil and Agricultural Chemistry Analysis[M].3rd ed.Beijing:China Agricultural Press, 2000.
[14]
GREGORY CAPORASO J, KUCZYNSKI J, STOMBAUGH J, et al.QIIME allows analysis of high-throughput community sequencing data[J].Nature Methods, 2010, 7(5):335-336.
[15]
WAWRIK B, KERKHOF L, KUKOR J, et al.Effect of different carbon sources on community composition of bacterial enrichments from soil[J].Appl Environ Microbiol, 2005, 71(11):6 776-6 783.
[16]
PHILIPPOT L, ANDERSSON S G E, BATTIN T J, et al.The ecological coherence of high bacterial taxonomic ranks[J].Nature Reviews Microbiology, 2010, 8(7):523-529.
[17]
杨敏, 殷绒, 张国涛, 等.基于高通量测序技术的香格里拉葡萄酒产区根际微生物多样性研究[J].云南农业大学学报(自然科学), 2020, 35(3):392-400.YANG M, YIN R, ZHANG G T, et al.Study on the grape rhizosphere microbial diversity in Shangri-La wine region by high-throughput sequencing technology[J].Journal of Yunnan Agricultural University (Natural Science), 2020, 35(3):392-400.
[18]
FIERER N, BRADFORD M A, JACKSON R B.Toward an ecological classification of soil bacteria[J].Ecology, 2007, 88(6):1 354-1 364.
[19]
HIRANO S S, UPPER C D.Bacteria in the leaf ecosystem with emphasis on Pseudomonas syringae-a pathogen, ice nucleus, and epiphyte[J].Microbiology and Molecular Biology Reviews, 2000, 64(3):624-653.
[20]
PARK J Y, HAN S H, LEE J H, et al.Draft genome sequence of the biocontrol bacterium Pseudomonas putida B001, an oligotrophic bacterium that induces systemic resistance to plant diseases[J].Journal of Bacteriology, 2011, 193(23):6 795-6 796.
[21]
雒晓芳, 陈俊楠, 田丹妮, 等.白色类诺卡氏菌的分离鉴定及其抗菌活性初探[J].中国酿造, 2015, 34(10):58-61.LUO X F, CHEN J N, TIAN D N, et al.Separation and identification of Nocardioides albus and preliminary research on its antibacterial activity[J].China Brewing, 2015, 34(10):58-61.
[22]
GONG A D, DONG F Y, HU M J, et al.Antifungal activity of volatile emitted from Enterobacter asburiae Vt-7 against Aspergillus flavus and aflatoxins in peanuts during storage[J].Food Control, 2019, 106:106 718.
[23]
姜怡, 杨颖, 陈华红, 等.植物内生菌资源[J].微生物学通报, 2005(6):146-147.JIANG Y, YANG Y, CHEN H H, et al.Plant endophyte resources[J].Microbiology China, 2005(6):146-147.
[24]
MAICAS S.The use of alternative technologies to develop malolactic fermentation in wine[J].Applied Microbiology and Biotechnology, 2001, 56(1-2):35-39.
[25]
CLAUDIO D, FORMICA J V.Wine Microbiology[M].Taylor and Francis, 2012-06-15.
[26]
ZABAT M A,SANO W H,WURSTER J I, et al.Microbial community analysis of sauerkraut fermentation reveals a stable and rapidly established community[J].Foods, 2018, 7(5):77.
[27]
RHEE S J, LEE J E, LEE C H.Importance of lactic acid bacteria in Asian fermented foods[J].Microbial Cell Factories, 2011, 10(S1):5.
[28]
KIM M J, LEE H W, LEE M E, et al.Mixed starter of Lactococcus lactis and Leuconostoc citreum for extending kimchi shelf-life[J].Journal of Microbiology, 2019, 57(6):479-484.
[29]
周利国. 宁夏银川葡萄酒产区酒酒球菌分离筛选及鉴定[D].杨凌:西北农林科技大学, 2009.ZHOU L G.The isolation and identification of Oenococcus oeni from Ningxia Yinchuan wine production region[D].Yangling:Northwest A&F University, 2009.