Effects of water extractions from strawberry leaves on COL I synthesis and BMP-1 secretion in HFF-1
DING Wenyu1,2, HE Congfen1,2, LIU Lei1,2, YANG Xiaoxiao1,2, Dong Kun1,2*
1(School of Chemical and Materials Engineering,Beijing Technology and Business University,Beijing 100048,China) 2(Beijing Key Lab of Plant Resources Research and Development,Beijing Technology and Business University,Beijing 100048,China)
Abstract: Water extractions from strawberry leaves(SLWE)contains a variety of bioactive components,which can stimulate fibroblasts to synthesize collagens.And collagens must be proteolytic cleavaged by bone morphogenetic protein 1(BMP-1) to turn into an active form.In this study,the bioactive component contents and main antioxidant enzyme activities of SLWE were analyzed.Furthermore,the effect of SLWE on collagen I (COL I) synthesis and BMP-1 secretion by human foreskin fibroblasts (HFF-1) was evaluated.The results showed that the contents of Vc,proanthocyanidins,polysaccharides,total flavonoids,total phenols and protein in SLWE were (0.88±0.025),(0.28±0.027),(10.00±0.278),(0.47±0.031),(0.10±0.003)mg/mL,respectively.SLWE also presented antioxidant activity with 125.59 U/mL,0.06 U/mL for SOD and POD,respectively.Though SLWE had no obvious effect on the proliferation of HFF-1 cells,it promoted COL I synthesis and secretion of BMP-1.Overall,our study indicated that SLWE contained abundant bioactive components and promoted the secretion and maturation of collagens in HFF-1 without increasing the cell number of HFF-1.Therefore,SLWE has been demonstrated as a potential functional ingredient for anti-aging cosmetics.Our research also provides theoretical basis for the reuse of agricultural wastes like strawberry leaves.
丁文玉,何聪芬,刘蕾,等. 草莓叶水提物对成纤维细胞合成Ⅰ型胶原及分泌骨形态发生蛋白-1的影响[J]. 食品与发酵工业, 2021, 47(9): 114-119.
DING Wenyu,HE Congfen,LIU Lei,et al. Effects of water extractions from strawberry leaves on COL I synthesis and BMP-1 secretion in HFF-1[J]. Food and Fermentation Industries, 2021, 47(9): 114-119.
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2021, Vol. 47 
