Abstract: L-Fucose is a rare monosaccharide with many important physiological functions, such as immune regulation, anti-infection and anti-tumor. The production of L-fucose mainly include plant extraction, chemical synthesis, and microbial fermentation, in which microbial fermentation has some advantages including effective productivity, high yield, and environmentally friendly. The hydrolysis of L-fucose from fucose-rich extracellular polysaccharide was an alternative process for L-fucose production. In this review, the fermentation process, metabolic engineering and extraction process of L-fucose produced by fermentation methods are discussed.
马巍,邹祥. 发酵法生产L-岩藻糖的研究进展[J]. 食品与发酵工业, 2021, 47(16): 308-312.
MA Wei,ZOU Xiang. Research progress on the production of L-fucose by fermentation[J]. Food and Fermentation Industries, 2021, 47(16): 308-312.
[1] 王永胜, 王硕, 张慧林, 等.L-岩藻糖对母乳寡糖(HMOs)合成的意义及其产业化研究进展[J].中国农学通报, 2019, 35(11):127-132. WANG Y S, WANG S, ZHANG H L, et al.L-Fucose:The significance to synthesis of human milk oligosaccharides (HMOs) and its research progress of industrialization[J].Chinese Agricultural Science Bulletin, 2019, 35(11):127-132. [2] 吴巍, 孙敬, 冯士超.HPLC-ELSD测定岩藻聚糖硫酸酯中L(-)岩藻糖[J].食品研究与开发, 2013, 34(21):90-92. WU W, SUN J, FENG S C.Determination of L-fucose in fucoidan extract by HPLC-ELSD[J].Food Research and Development, 2013, 34(21):90-92. [3] LISTINSKY J J, SIEGAL G P, LISTINSKY C M.The emerging importance of α-L-fucose in human breast cancer:A review[J].American Journal of Translational Research, 2011, 3(4):292-322. [4] LISTINSKY J J, LISTINSKY C M, Alapati V, et al.Cell surface fucose ablation as a therapeutic strategy for malignant neoplasms[J].Anatomic Pathology, 2001, 8(6):330-337. [5] SHETTY R K S, BHANDARY S K, KALI A.Significance of serum L-fucose glycoprotein as cancer biomarker in head and neck malignancies without distant metastasis[J].Journal of Clinical and Diagnostic Research, 2013, 7(12):2 818-2 820. [6] KATO J, TAKIMOTO R, OSUGA T,et al.Targeting SN38 delivery to gastrointestinal cancer cells using a fucose-bound nanoparticle approach[C].Proceedings of the 105th Annual Meeting of the American Association for Cancer Research,San Diego:Cancer Research, 2014. [7] YOSHIDA M, TAKIMOTO R, MURASE K, et al.Targeting anticancer drug delivery to pancreatic cancer cells using a fucose-bound nanoparticle approach[J].PLoS One, 2012, 7(7):e39 545. [8] FODIL B I, BIZBIZ L, SCHOEVAERT D, et al.Effect of L-fucose and fucose-rich oligo- and polysaccharides (FROP-s) on skin aging:Penetration, skin tissue production and fibrillogenesis[J].Biomed Pharmacother, 2003, 57(5):209-215. [9] WIJESINGHE W, JEON Y J.Biological activities and potential industrial applications of fucose rich sulfated polysaccharides and fucoidans isolated from brown seaweeds:A review[J].Carbohydrate Polymers, 2012, 88(1):13-20. [10] 余华. 海带多糖提取条件的优化和脱蛋白研究[J].中国食品添加剂, 2006(3):39-43. YU H.Study on the extracting condition and deproteinization of laminaria japonica aresch polysaccharide[J].China Food Additives, 2006(3):39-43. [11] VANHOOREN P T,VANDAMME E J.L-Fucose:Occurrence,physiological role,chemical,enzymatic and microbial synthesis[J].Cheminform, 1999, 30(33):479-497. [12] 黄宜兰, 王岩, 陈少欣.L-岩藻糖的生产菌种选育及发酵工艺优化[J].中国医药工业杂志, 2015, 46(8):823-826. HUANG Y L, WANG Y, CHEN S X.Strain screening and fermentation production of L-fucose by Enterobacter sp.[J].Chinese Journal of Pharmaceuticals, 2015, 46(8):823-826. [13] FREITAS F,ALVES V D,GOUVEIA A R, et al.Controlled production of exopolysaccharides from Enterobacter A47 as a function of carbon source with demonstration of their film and emulsifying abilities[J].Applied Biochemistry & Biotechnology, 2014, 172(2):641-657. [14] ALVES V D, FREITAS F,TORRES C A V, et al.Rheological and morphological characterization of the culture broth during exopolysaccharide production by Enterobacter sp.[J].Carbohydrate Polymers, 2010, 81(4):758-764. [15] TORRES C A V,MARQUES R, ANTUNES S, et al.Kinetics of production and characterization of the fucose-containing exopolysaccharide from Enterobacter A47[J].Journal of Biotechnology, 2011, 156(4):261-267. [16] TORRES C A V, ANTUNES S,RICARDO A R, et al.Study of the interactive effect of temperature and pH on exopolysaccharide production by Enterobacter A47 using multivariate statistical analysis[J].Bioresource Technology, 2012, 119:148-156. [17] TORRES C A V, MARQUES R,FERREIRA A R V, et al.Impact of glycerol and nitrogen concentration on Enterobacter A47 growth and exopolysaccharide production[J].International Journal of Biological Macromolecules, 2014, 71:81-86. [18] ANTUNES S,FREITAS F,ALVES V D, et al.Conversion of cheese whey into a fucose-and glucuronic acid-rich extracellular polysaccharide by Enterobacter A47[J].Journal of Biotechnology, 2015, 210:1-7. [19] ANTUNES S,FREITAS F,SEVRIN C, et al.Production of fucopol by Enterobacter A47 using waste tomato paste by-product as sole carbon source[J].Bioresource Technology, 2017, 227:66-73. [20] NIU S F, MA W, JIN M Y, et al.Complete genome sequence of Kosakonia sp.strain CCTCC M2018092, a fucose-rich exopolysaccharide producer[J].Microbiology Resource Announcements, 2019, 8(30).DOI:10.1128/MAR.00567-19. [21] VLAEV S D, STAYKOV P, POPOV R.Pressure distribution at impeller blades of some radial flow impellers in saccharose and xanthan gum solutions-A CFD visualization approach[J].Food and Bioproducts Processing, 2004, 82(C1):13-20. [22] HUANG J, JIANG S, XU X Q, et al.Effects of carbon/nitrogen ratio, dissolved oxygen and impeller type on gellan gum production in Sphingomonas paucimobilis [J].Ann Microbiol, 2012, 62(1):299-305. [23] 曾真,彭坤,王为国.发酵罐搅拌轴的优化设计[J].食品与机械, 2010, 26(6):97-100. ZENG Z, PENG K, WANG W G.Optimum design of the agitating shaft for the fermentation tank[J].Food & Machinery, 2010, 20(6):97-100. [24] HARWEY P S, GREAVES M.Turbulent flow in an agitated vessel.part I:A predictive model[J].Transactions of the Institution Chemical Engineer, 1982, 60:195-200. [25] OLDSHUE J Y.Fluid mixing in fermentation processes[J].Industrial & Engineering Chemistry Research, 1960, 2:275-287. [26] ZHAO H B, FEI X N, ZHANG B L, et al.Controlling the size of fragrance microcapsules using designed agitator paddles:Experiment and CFD simulation[J].Particuology, 2019, 43:38-45. [27] 张淑华, 李涛, 朱炳辰, 等.三相机械搅拌反应器气液传质[J].化工学报, 2005, 56(2):220-226. ZHANG S H, LI T, ZHU B C, et al.Gas-liquid mass transfer in three-phase mechanical agitated reactor[J].CIESC Journal, 2005, 56(2):220-226. [28] 袁海荣, 贾绍义.搅拌桨形式对盐酸四环素发酵的影响[J].西北药学杂志, 2005, 20(6):255-257. YUAN H R, JIA S Y.The effect of stirring blade on the fermentation of tetracycline hydrochloride[J].Northwest Pharmaceutical Journal, 2005, 20(6):255-257. [29] 郑之明, 杨守志, 樊永红, 等.黄原胶发酵过程中流变学行为和气液传质的研究[J].天然气化工, 2006(5):27-31. ZHENG Z M, YANG S Z, FAN Y H, et al.Studies on fluid rheological properties and mass transfer during the fermentation of xanthomonas campestris in a stired reactor[J].Natural Gas Chemical Industry, 2006(5):27-31. [30] HALFI E, ARAD A, BRENNER A, et al.Development of an oscillation-based technology for the removal of colloidal particles from water:CFD modeling and experiments[J].Engineering Applications of Computational Fluid Mechanics, 2020, 14(1):622-641. [31] YAO M, RAN Z, CHEN T, et al.Numerical simulation studies of agitating paddle dependence on characteristics of the flow field in the mechanical-L-continuous-flow-stirred reactor for flocculation[J].Desalination and Water Treatment, 2020, 183:30-41. [32] SCHMID J, SIEBER V, REHM B.Bacterial exopolysaccharides:Biosynthesis pathways and engineering strategies[J].Frontiers in Microbiology, 2015, 6:496. [33] YI W, LIU X W, LI Y H, et al.Remodeling bacterial polysaccharides by metabolic pathway engineering[J].Proceedings of the National Academy of Sciences of the United States of America, 2009, 106(11):4 207-4 212. [34] BYUN S G, KIM M D, LEE W H, et al.Production of GDP-L-fucose, L-fucose donor for fucosyloligosaccharide synthesis, in recombinant Escherichia coli[J].Applied Microbiology Biotechnology, 2007, 74(4):768-775. [35] LEE W H, HAN N S, PARK Y C, et al.Modulation of guanosine 5′-diphosphate-D-mannose metabolism in recombinant Escherichia coli for production of guanosine 5′-diphosphate-L-fucose[J].Bioresource Technology, 2009, 100(24):6 143-6 148. [36] LEE W H, CHIN Y W, HAN N S, et al.Enhanced production of GDP-L-fucose by overexpression of NADPH regenerator in recombinant Escherichia coli[J].Applied Microbiology and Biotechnology, 2011, 91:967-976. [37] HUANG D, YANG K X, LIU J, et al.Metabolic engineering of Escherichia coli for the production of 2′-fucosyllactose and 3-fucosyllactose through modular pathway enhancement[J].Metabolic Engineering, 2017, 41:23-38. [38] MATTILA P, RABINA J, HORTLING S, et al.Functional expression of Escherichia coli enzymes synthesizing GDP-L-fucose from inherent GDP-D-mannose in Saccharomyces cerevisiae[J].Glycobiology, 2000, 10(10):1 041-1 047. [39] CHIN Y W, PARK J B, PARK Y C, et al.Metabolic engineering of Corynebacterium glutamicum to produce GDP-L-fucose from glucose and mannose[J].Bioprocess and Biosystems Engineering, 2013, 36(6):749-756. [40] KOIZUMI S, ENDO T, TABATA K, et al.Large-scale production of GDP-fucose and Lewis X by bacterial coupling[J].Journal of Industrial Microbiology & Biotechnology, 2000, 25(4):213-217. [41] LI L, KIM S A, HEO J E, et al.One-pot synthesis of GDP-L-fucose by a four-enzyme cascade expressed in Lactococcus lactis[J].Journal of Biotechnology, 2017, 264:1-7. [42] LIU J J, LEE J W, YUN E J, et al.L-Fucose production by engineered Escherichia coli[J].Biotechnology and Bioengineering, 2019, 116(4):904-911. [43] 程仕伟,陈超男,冯志彬,等.海带岩藻多糖的水提制备及其抗氧化活性研究[J].食品科学, 2010, 31(6):101-104. CHENG S W, CHEN C N, FENG Z B, et al.Water extraction and antioxidant activity of fucoidan from Laminaria japonica[J].Food Science, 2010, 31(6):101-104. [44] GORI A, BIAGIOLINI S, MANONI M, et al.Process for production of L-fucose:WO/2012/034996A1[P].2012-03-22. [45] LI S S, XIA H Q, XIE A Q, et al.Structure of a fucose-rich polysaccharide derived from EPS produced by Kosakonia sp.CCTCC M2018092 and its application in antibacterial film[J].International Journal of Biological Macromolecules, 2020, 159:295-303. [46] HE R H, LI Y, HAN C Q, et al.L-fucose ameliorates DSS-induced acute colitis via inhibiting macrophage M1 polarization and inhibiting NLRP3 inflammasome and NF-κB activation[J].International Immunopharmacology, 2019, 73:379-388. [47] WIERICHS R J, ZELCK H, DOERFER C E, et al.Effects of dentifrices differing in fluoride compounds on artificial enamel caries lesions in vitro[J].Odontology, 2017, 105(1):36-45. [48] DONALDSON G P, LADINSKY M S, YU K B, et al.Gut microbiota utilize immunoglobulin A for mucosal colonization[J].Science, 2018, 360(6 390):795-800.
2021, Vol. 47 
