Screening,identification and enzyme characteristics of alginate lyase-producing strains from the gut of Monodonta labio
LIU Yaotian1,2, XI Maosheng1,2, ZHAO Yang1,2, LI Yuechan1,2, LUO Xuegang1,2*
1(College of Bioengineering,Tianjin University of Science and Technology,Tianjin 300457,China) 2(Key Laboratory of Industrial Fermentation Microbiology of the Ministry of Education,Tianjin University of Science and Technology,Tianjin 300457,China)
Abstract: High-yield alginate lyase producing strain ML17 was screened from the intestinal tract of Monodonta labio in the North-South intertidal zone of China by sodium alginate as the sole carbon source. The strain was identified as Vibrio sp. ML17 (CGMCC NO. 20377) by morphological analysis and 16S rRNA. The results of Wagstsuma blood agar plate analysis showed that ML17 was non-hemolytic. Single factor results showed that ML17 optimal conditions as follows: sodium alginate of 5 g/L, tryptone of 5 g/L, NaCl of 30 g/L, pH 7-8 and 30 ℃. Vibrio sp. ML17 reached the growth plateau after 10 h under the optimal conditions, and the highest enzyme production was achieved after 14 h. In addition, the activity of alginate lyase was 26.31 U/mL. The optimal temperature pH was 40 ℃ and 7.5, respectively. It had certain thermal-stability at 40 ℃ and 45 ℃. Under the condition of 5 mmol/L Na+ and Ca2 +, the activity was significantly promoted, while Fe2 +, Cu2 + and Zn2 + inhibited. Further application results showed that Vibrio sp. ML17 could effectively degrade brown algae, and the degradation rates of kelp and sargassum reached 44.0% and 38.9% after 72 h, respectively. Vibrio sp. ML17 could have good application potential in the preparation of alginate oligo-saccharides and the control and removal of alginate in aquaculture.
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