Breeding of tannase-producing Aspergillus carbonarius using ARTP mutagenesis and fermentation optimization
DONG Xianxian1,2,3, WU Xiaojiang1,2,3, ZHANG Yulong1,2,3, WU Xiaodan1,2,3, WAN Yin1,2,3, LIU Chengmei1,2,3, FU Guiming1,2,3*
1(State Key Laboratory of Food Science and Technology,Nanchang University,Nanchang 330000,China) 2(College of Food Science & Technology,Nanchang University,Nanchang 330000,China) 3(International Institute of Food Innovation,Nanchang University,Nanchang 330000,China)
Abstract: In order to obtain high yield of tannase, atmospheric room temperature plasma (ARTP) was used to mutagenize Aspergillus carbonarius FCYN212 and fermentation parameters were optimized. The results showed that NCUF M8 with high tannase activity was successfully selected after ARTP mutagenesis, bromophenol blue circle screening and fermentation. The tannase activity of NCUF M8 was 0.212 U/mL, which was 57% higher than that of parent strain (0.135 U/mL). After eight consecutive generations and fermentation tests, the tannase activity levels were kept at 0.208 U/mL, which indicated that it had a good genetic stability. The optimal fermentation conditions were as followed: fermentation time of 4 d, inoculation of 2%, tannic acid of 60 g/L, dissolved oxygen concentration of 45%, and an addition time of inducer at 36 h. The tannase activity of NCUF M8 reached 1.377 U/mL under the optimized conditions, which was 549% significantly higher than original strain of 0.212 U/mL (P<0.05). The results provided a theoretical basis for the mutation breeding of tannase producing strain. Besides, it could reduce the production cost of tannase industrial production.
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