Abstract: In order to study the regulatory effect of fruit mulberry tea on intestinal microbiota in type 2 diabetes mice model, high-fat diet combined with streptozotocin was used to induce the type 2 diabetic mice model. The feces of mice from different groups were collected to analyze the changes in the microbiota using high-throughput sequencing. The results showed that the occurrence of type 2 diabetes could lead to the imbalance of intestinal microbiota in mice, among which the diversity of intestinal microbiota in the control group was the lowest, the relative abundance of some beneficial bacteria was decreased, and the relative abundance of harmful bacteria was increased. In contrast, fruit mulberry tea powder dose group (GDm group) and fruit mulberry tea extract dose group (GDh group) significantly increased the relative abundance of Muribaculaceae_unclassified, Akkermansia, Lachnospiraceae and other beneficial bacteria, enriching the diversity of bacteria. The intestinal microbiota of the GDm group was the closest to that of the normal control group, and the regulation effect was the most significant. This research proves that fruit mulberry tea can effectively regulate the structure and abundance of intestinal microbiota in type 2 diabetes model mice, promote the benign development of intestinal microbiota, providing theoretical basis for the development of fruit mulberry hypoglycemic health products.
魏建敏,杨华连,陈莉,等. 基于高通量测序分析果桑茶对2型糖尿病模型小鼠肠道菌群的影响[J]. 食品与发酵工业, 2021, 47(20): 75-82.
WEI Jianmin,YANG Hualian,CHEN Li,et al. Effect of fruit mulberry tea on intestinal microbiota of type 2 diabetic mice based on high-throughput sequencing[J]. Food and Fermentation Industries, 2021, 47(20): 75-82.
[1] 王娜, 林彩霞, 徐李玲, 等.青钱柳双瓜袋泡茶对2型糖尿病小鼠血糖、血脂与肝肾功能的影响及机制研究[J].广西医科大学学报, 2019, 36(12):1 889-1 894. WANG N, LIN C X, XU L L, et al.Effects and mechanisms of Paliurus Duplex Cucumis tea bags on blood glucose, serum lipid and liver and kidney function in type 2 diabetic mice[J].Journal of Guangxi Medical University, 2019, 36(12):1 889-1 894. [2] 何静, 高婉婷, 海勒, 等.驼乳对2型糖尿病小鼠肝脏损伤的保护作用[J].中国食品学报, 2019, 19(7):36-41. HE J, GAO W T, HAI L, et al.Protective effect of camel milk on liver injury in type 2 diabetic mice[J].Journal of Chinese Institute of Food Science and Technology, 2019, 19(7):36-41. [3] WANG L M, GAO P, ZHANG M, et al.Prevalence and ethnic pattern of diabetes and prediabetes in China in 2013[J].JAMA, 2017, 317(24):2 515-2 523. [4] HOLMAN N, YOUNG B, GADSBY R.Current prevalence of type 1 and type 2 diabetes in adults and children in the UK[J].Diabetic Medicine, 2015, 32(9):1 119-1 120. [5] MOUSTAFA P E, ABDELKADER N F, AWDAN S A, et al.Extracellular matrix remodeling and modulation of inflammation and oxidative stress by sulforaphane in experimental diabetic peripheral neuropathy[J].Inflammation, 2018, 41(4):1 460-1 476. [6] 焦丹, 李浩霞, 吴丹丹, 等.魔芋低聚糖缓解高糖水平诱发的大鼠代谢综合征及相关机制[J].食品科学, 2019,40(13):137-142. JIAO D, LI H X, WU D D, et al.Alleviatory effect and underlying mechanism of konjac oligosaccharides on high glucose induced-metabolic syndrome in rats[J].Food Science, 2019, 40(13):137-142. [7] SAGBO I J, VAN DE VENTER M, KOEKEMOER T, et al.In vitro antidiabetic activity and mechanism of action of Brachylaena elliptica (thunb.) DC[J].Evidence-based Complementary and Alternative Medicine:ECAM, 2018.DOI:10.1155/2018/4170372. [8] 亓桂成, 王翠香, 韩朝晖, 等.不同处理方法对台湾果桑硬枝扦插的影响[J].山东林业科技, 2018, 48(3):49-51;56. QI G C, WANG C X, HAN Z H, et al.Effects of different treatment methods on hardwood cutting of mulberry in Taiwan[J].Journal of Shandong Forestry Science and Technology, 2018, 48(3):49-51;56. [9] 姜贝贝, 罗阳, 王洪荣.桑叶的营养价值及作为畜禽饲料的研究进展[J].饲料工业, 2017, 38(5):51-54. JIANG B B, LUO Y, WANG H R.The nutritional value of mulberry leaf and its application in the livestock feed[J].Feed Industry, 2017, 38(5):51-54. [10] 余婉莎. 桑枝多糖提取与结构分析及生物活性探究[D].重庆:西南大学, 2018. YU W S.Extraction, structure analysis and biological activity of polysaccharides from ramulus mori[D].Chongqing:Southwest University, 2018. [11] 段志涛. 桑白皮有效部位工艺、质控及药效学研究[D].广州:广州中医药大学, 2013. DUAN Z T.Study on extraction, purification and quality standard research of the total effective parts of cortex mori and discussing on lowering blood sugar and blood lipid[D].Guangzhou:Guangzhou University of Chinese Medicine, 2013. [12] LIU Q P, LI X, LI C Y, et al.1-deoxynojirimycin alleviates insulin resistance via activation of insulin signaling PI3K/AKT pathway in skeletal muscle of db/db mice[J].Molecules, 2015, 20(12):21 700-21 714. [13] 郭丽璇, 胡琼英, 熊大迁.肠道菌群调控2型糖尿病发生发展的研究进展[J].实用医学杂志, 2020, 36(9):1 142-1 147. GUO L X, HU Q Y, XIONG D Q.Research progresses of intestinal flora regulating the occurrence and the development of type 2 diabetes mellitus[J].The Journal of Practical Medicine, 2020, 36(9):1 142-1 147. [14] PRAKASH S, RODES L, COUSSA-CHARLEY M, et al.Gut microbiota:Next frontier in understanding human health and development of biotherapeutics[J].Biologics, 2011, 5:71-86. [15] 李沫. 普洱茶通过作用于肠道菌群从而对糖尿病前期患者糖脂代谢影响的机制研究[D].长春:吉林大学, 2020. LI M.Mechanism of Puer tea improving glucose and lipid metabolism in pre-diabetic patients by effecting on intestinal microflora[D].Changchun:Jilin University, 2020. [16] ZHAO L P, ZHANG F, DING X Y, et al.Gut bacteria selectively promoted by dietary fibers alleviate type 2 diabetes[J].Science, 2018, 359(6 380):1 151-1 156. [17] MORENO-INDIAS I, CARDONA F, TINAHONES F J, et al.Impact of the gut microbiota on the development of obesity and type 2 diabetes mellitus[J].Frontiers in Microbiology, 2014, 5:190. [18] LARSEN N, VOGENSEN F K, VAN D BERG F W J, et al.Gut microbiota in human adults with type 2 diabetes differs from non-diabetic adults[J].PloS One, 2010, 5(2):e9085. [19] SEDIGHI M, RAZAVI S, NAVAB-MOGHADAM F, et al.Comparison of gut microbiota in adult patients with type 2 diabetes and healthy individuals[J].Microbial Pathogenesis,2017, 111:362-369. [20] 何雪冬, 王芳, 王瑶, 等.糖尿病患者肠道菌群特征及其相关性的系统评价[J].中国微生态学杂志, 2020, 32(4):397-403. HE X D, WANG F, WANG Y, et al.Characteristics of intestinal flora in diabetic patients and their correlation:A systematic review[J].Chinese Journal of Microecology, 2020, 32(4):397-403. [21] CALLAHAN B J, MCMURDIE P J, ROSEN M J, et al.DADA2:High-resolution sample inference from illumina amplicon data[J].Nature Methods, 2016, 13(7):581-583. [22] 孙鹏鹏. 益生菌发酵枇杷提取物的功效学研究[D].济南:山东大学, 2019. SUN P P.Study on the efficacies of probiotics fermented loquat extracts[D].Jinan:Shandong University, 2019. [23] SHENG Y,ZHENG S J,ZHANG C H, et al.Mulberry leaf tea alleviates diabetic nephropathy by inhibiting PKC signaling and modulating intestinal flora[J].Journal of Functional Foods, 2018, 46:118-127. [24] BUNKER J J, DREES C, WATSON A R, et al.B cell superantigens in the human intestinal microbiota[J].Science Translational Medicine, 2019, 11(507):e9356. [25] YAN X, YANG C F, LIN G P, et al.Antidiabetic potential of green seaweed enteromorpha prolifera flavonoids regulating insulin signaling pathway and gut microbiota in type 2 diabetic mice[J].Journal of Food Science, 2019,84(1):165-173. [26] BEZIRTZOGLOU E,TSIOTSIAS A,WELLING G W.Microbiota profile in feces of breast and formula-fed newborns by using fluorescence in situ hybridization (FISH)[J].Anaerobe, 2011, 17(6):478-482. [27] QIN J J, LI R Q, RAES J, et al.A human gut microbial gene catalogue established by metagenomic sequencing[J].Nature, 2010, 464(7 285):59-65. [28] 刘尔卓, 吴忠坤, 赵紫薇, 等.基于高通量测序分析鲜槟榔水提液对于小鼠肠道菌群及免疫指标的影响[J].食品研究与开发, 2020, 41(10):28-33;166. LIU E Z, WU Z K, ZHAO Z W, et al.High-throughput sequencing analysis of fresh Areca nut extract on intestinal flora and immune indexes of mice[J].Food Research and Development, 2020, 41(10):28-33;166. [29] FENG Z, LONG W M, HAO B H, et al.A human stool-derived bilophila wadsworthia strain caused systemic inflammation in specific-pathogen-free mice[J].Gut Pathogens, 2017, 9(1):59. [30] CAN I P D,DE VOS W M.Next-generation beneficial microbes:The case of Akkermansia muciniphila[J].Frontiers in Microbiology, 2017, 8:1 765. [31] 杨莉, 杨卫星, 张智芳, 等.高通量测序研究德昂酸茶对高脂饮食小鼠肠道微生物的影响[J].扬州大学学报(农业与生命科学版), 2020, 41(2):100-106. YANG L, YANG W X, ZHANG Z F, et al.Effect of De′ang pickled tea on intestinal microorganisms in mice with high- fat diet based on high-throughput sequencing[J].Journal of Yangzhou University(Agricultural and Life Science Edition), 2020, 41(2):100-106. [32] GUO X J, CHENG M, ZHANG X, et al.Green tea polyphenols reduce obesity in high-fat diet-induced mice by modulating intestinal microbiota composition[J].International Journal of Food Science & Technology, 2017,52(8):1 723-1 730.
2021, Vol. 47 
