Effect of fermented hemp seed protein on the cellular oxidative stress with HepG2 cellular model
LI Haizhi1,2, Wang Shubin3, GUO Shanshan3, YU Youqiang1,2, LIU Yifeng1,2, PAN Cong1,2, WU Yimin1,2, ZHOU Zhiqiao1,2, XIA Kai1,2*, LI Yali1,2*
1(China National Research Institute of Food Fermentation Industries Co.Ltd.,Beijing 100015,China) 2(Functional Staple Food Creation and Nutrition Intervention for Chronic Diseases in Beijing Key Laboratory,Beijing 100015,China) 3(Hanyi Biotechnology (Beijing) Co.Ltd.,Beijing 100020,China)
Abstract: To evaluate the antioxidant capacity of fermented hemp seed protein powder, oxidative stress of HepG2 cells was induced by 2,2′-azobis(2-methylpropionamidine)dihydrochloride (AAPH) to establish the model. The antioxidant activity was measured by reactive oxygen species (ROS), malondialdehyde (MDA), the activities of superoxide dismutase (SOD), glutathione peroxidase (GSH-Px),and the expression of antioxidant related genes in HepG2 cells. The results showed that the activities of SOD and GSH-Px were significantly increased (P<0.05), while ROS and MDA were significantly reduced (P<0.05) after the treatment with fermented hemp seed protein powder. The expression levels of NQO1, Nrf2 and HO-1 mRNA in fermented hemp seed protein powder were increased. The results showed that the Nrf2 pathway was activated by fermented hemp seed protein powder to alleviate the oxidative stress damage of HepG2 induced by AAPH.
李海枝,王曙宾,郭珊珊,等. 发酵火麻仁蛋白粉对HepG2细胞氧化应激损伤的保护作用[J]. 食品与发酵工业, 2021, 47(15): 84-89.
LI Haizhi,Wang Shubin,GUO Shanshan,et al. Effect of fermented hemp seed protein on the cellular oxidative stress with HepG2 cellular model[J]. Food and Fermentation Industries, 2021, 47(15): 84-89.
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2021, Vol. 47 
