Abstract: To improve production efficiency of 2-phenylethanol (2PE) from L-Pheby Metschnikowia sp., a high-yield 2PE strain CICC 1467 was screened from four candidate Metschnikowia strains. Then, factors were investigated to enhance the production of 2PE, including L-Phe dosage, carbon source, initial pH, temperature, and inoculation volume. Furthermore, the fermentation ability of CICC 1467 under non-sterile conditions was investigated. Under optimal conditions, the final 2PE and molar yield reached 2.24 g/L and 0.76, respectively. The condition were as follows: L-Phe 4 g/L, glucose 50 g/L, initial pH 3.0, 25℃, inoculation volume 5%, 44 h, and with non-sterilized tap water as the solvent. The results represent the highest molar yield compared with the ever reported of 2PE from L-Phe by Metschnikowia. The study proved that Metschnikowia sp. had the ability to synthesize 2PE efficiently, and important reference value for microbial synthesis of 2PE.
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