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食品与发酵工业  2021, Vol. 47 Issue (21): 102-108    DOI: 10.13995/j.cnki.11-1802/ts.026312
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共轭亚油酸甘油酯及其微囊乳液对高脂饮食诱导的大鼠非酒精性脂肪肝的缓解作用
文瑾, 范超, 夏俪宁, 石超, 吴文忠*
(大连医诺生物股份有限公司,辽宁 大连,116600)
Amelioration effects of conjugated linoleic acid glyceride and microencapsulated emulsion on high-fat diet induced non-alcoholic fatty liver disease in rats
WEN Jin, FAN Chao, XIA Lining, SHI Chao, WU Wenzhong*
(INNOBIO, Dalian 116600, China)
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摘要 为研究共轭亚油酸甘油酯(conjugated linoleic acid glyceride, CLA-TG)及其微囊化乳液对高脂饮食诱导的非酒精性脂肪肝(non-alcoholic fatty liver disease NAFLD)相关指标的影响,采用6周高脂饮食建立大鼠NAFLD模型,通过CLA-TG或微囊化CLA-TG干预,记录体重、摄食量,造模结束后收集肝脏及血清,检测血清学指标谷丙转氨酶(alanine aminotransferase,ALT)、谷草转氨酶(aspartate aminotransferase,AST)、总胆固醇(total cholesterol, TC)、低密度脂蛋白胆固醇(low-density lipoprotein cholesterol, LDL-C)、高密度脂蛋白胆固醇(high-density lipoprotein cholesterol, HDL-C)及甘油三酯(triglycerides, TG)水平;检测肝脏TG含量及油红O染色观察脂滴积聚情况。结果表明,各组大鼠实验周期内摄食量无较大差异。与空白对照组相比,模型组大鼠体重、血脂水平显著升高,肝脏出现明显的肝脂肪变,表现为显著的TG累积及脂滴积聚。CLA-TG及微囊化CLA-TG的摄入使上述指标均明显缓解,且微囊化CLA-TG组改善肝损伤效果更为显著。CLA-TG的摄入可对NAFLD起到显著的缓解作用,且微囊化后缓解作用进一步增强。
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文瑾
范超
夏俪宁
石超
吴文忠
关键词:  非酒精性脂肪肝  共轭亚油酸甘油酯  微囊  肥胖  脂质代谢    
Abstract: This study aimed to investigate the effects of conjugated linoleic acid glyceride oil (CLA-TG) and microencapsulated CLA-TG emulsion on high-fat diet induced non-alcoholic fatty liver disease in rats.Male Sprague-Dawley rats were fed with high-fat diet for 6 weeks to establish the non-alcoholic fatty liver disease model.At the same time, the intervention groups were fed with CLA-TG oil and microencapsulated CLA-TG emulsion. Body weight and food intake were recorded.After 6 weeks treatment, the liver and blood were collected to exam the liver function indicators alanine aminotransferase (ALT), aspartate aminotransferase (AST), total cholesterol (TC), triglycerides (TG), low-density lipoprotein cholesterol (LDL-C), high-density lipoprotein cholesterol (HDL-C) in serum and triglycerides (TG) in liver. And oil-red O staining was used to observe the lipid accumulation of liver tissue. Results showed that the food intake of all groups showed no significant difference. Compared with the control group, the body weight in the model group increased significantly (P<0.01). The levels of serum ALT、AST、TC、TG、LDL-C were also significantly elevated (P<0.01) and HDL-C were significantly decreased (P<0.05). Sever steatosis were observed in the liver tissue which showed excessive TG and lipid accumulation (P<0.01).Compared with the model group, body weight, serum blood lipids levels, liver TG and lipid accumulation were significantly lower in CLA-TG group and microencapsulated CLA-TG emulsion groups (P<0.05). Moreover, the microencapsulated CLA-TG emulsion group showed better amelioration effects. The ingestion of CLA-TG can improve non-alcoholic fatty liver disease in rats and microencapsulated CLA-TG emulsion can further enhance the protective effects.
Key words:  non-alcoholic fatty liver disease    conjugated linoleic acid glyceride (CLA-TG)    microcapsule    obesity    lipid metabolism
收稿日期:  2020-11-29      修回日期:  2021-01-25           出版日期:  2021-11-15      发布日期:  2021-11-30      期的出版日期:  2021-11-15
作者简介:  硕士,药师(吴文忠研究员为通讯作者,E-mail:wu@innobio.cn)
引用本文:    
文瑾,范超,夏俪宁,等. 共轭亚油酸甘油酯及其微囊乳液对高脂饮食诱导的大鼠非酒精性脂肪肝的缓解作用[J]. 食品与发酵工业, 2021, 47(21): 102-108.
WEN Jin,FAN Chao,XIA Lining,et al. Amelioration effects of conjugated linoleic acid glyceride and microencapsulated emulsion on high-fat diet induced non-alcoholic fatty liver disease in rats[J]. Food and Fermentation Industries, 2021, 47(21): 102-108.
链接本文:  
http://sf1970.cnif.cn/CN/10.13995/j.cnki.11-1802/ts.026312  或          http://sf1970.cnif.cn/CN/Y2021/V47/I21/102
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