Please wait a minute...
 
 
食品与发酵工业  2021, Vol. 47 Issue (15): 77-83    DOI: 10.13995/j.cnki.11-1802/ts.026448
  研究报告 本期目录 | 过刊浏览 | 高级检索 |
四株乳杆菌作为口腔益生菌的特性研究
余意1,2, 王超越1,2, 吴正钧2*, 张佳2, 吴天赐2
1(上海海洋大学 食品学院,上海,201306)
2(光明乳业股份有限公司乳业生物技术国家重点实验室,上海乳业生物工程技术中心,上海,200436)
Potentials of Lactobacillus strains as oral probiotics candidates
YU Yi1,2, WANG Chaoyue1,2, WU Zhengjun2*, ZHANG Jia2, WU Tianci2
1(College of Food Science and Technology,Shanghai Ocean University,Shanghai 201306,China)
2(Research Institute,Bright Dairy and Food Co.Ltd.,Shanghai 200436,China)
下载:  HTML  PDF (2873KB) 
输出:  BibTeX | EndNote (RIS)      
摘要 以不同来源分离得到的4株乳杆菌为实验对象,通过体外试验,对其疏水作用、静电作用、聚集能力、耐溶菌酶能力、自身形成生物膜的能力和消除口腔致病菌变异链球菌(Streptococcus mutans)产生口腔生物膜的能力进行评价,发现4株乳杆菌都具有较高的自聚集能力(37.67%~60.89%)和S.mutans共聚的作用,其中Lacticaseibacillus rhamnosus B6、Limosilactobacillus fermentum B44、Lacticaseibacillus casei LC2W和Lactiplantiacillus plantarum ST-III共聚集能力分别可达到63.47%、46.42%、60.5%和61.51%;并且4株乳杆菌都具有一定的静电作用(≥15.86%)和疏水作用(≥77.24%),有利于在口腔内的黏附;都能耐受1 mg/mL的溶菌酶,具有较强的存活能力;相比于致病菌S.mutans,自身生物膜形成能力都较弱,不会促进口腔生物膜的形成;并且在与S.mutans共培养过程中,可以有效抑制S.mutans生物膜的形成,抑制率达到31.04%~54.28%。综合而言,4株乳杆菌都具备一定的预防龋病的潜力。
服务
把本文推荐给朋友
加入引用管理器
E-mail Alert
RSS
作者相关文章
余意
王超越
吴正钧
张佳
吴天赐
关键词:  乳杆菌  变异链球菌  龋齿  生物膜  胞外多糖    
Abstract: Four Lactobacillus strains were evaluated in vitro by demonstrate high hydrophobicity, electrostatic charge, tolerance to lysozyme and lower biofilm formation ability. The auto-aggregation ability of four tested Lacticaseibacillus rhamnosus B6, Limosilactobacillus fermentum B44, Lacticaseibacillus casei LC2W and Lactiplantiacillus plantarum ST-III were 37.67%-60.89%, while their co-aggregating with Streptococcus mutans, the predominant pathogen causing dental caries in oral cavity, reached 63.47%, 46.42%, 60.5% and 61.51%, respectively. When cocultured with S. mutans in BHI containing 3 g/L sucrose, they could reduce the formation of S. mutans biofilm by 31.04%-54.28%, which might be attributed to synergetic effect of organic acids as well as other antagonistic substances produced by Lactobacilli. In conclusion, four tested strains have potentials as oral probiotics candidates in preventing dental caries.
Key words:  Lactobacillus    Streptococcus mutans    dental caries    biofilm    exopolysaccharides
收稿日期:  2020-12-21      修回日期:  2020-12-30           出版日期:  2021-08-15      发布日期:  2021-08-23      期的出版日期:  2021-08-15
基金资助: 国家重点计划研发课题项目(2019YFF0217603);上海乳业生物工程技术研究中心项目(19DZ2281400)
作者简介:  硕士研究生(吴正钧教授级高级工程师为通讯作者,E-mail:wuzhengjun@brightdairy.com)
引用本文:    
余意,王超越,吴正钧,等. 四株乳杆菌作为口腔益生菌的特性研究[J]. 食品与发酵工业, 2021, 47(15): 77-83.
YU Yi,WANG Chaoyue,WU Zhengjun,et al. Potentials of Lactobacillus strains as oral probiotics candidates[J]. Food and Fermentation Industries, 2021, 47(15): 77-83.
链接本文:  
http://sf1970.cnif.cn/CN/10.13995/j.cnki.11-1802/ts.026448  或          http://sf1970.cnif.cn/CN/Y2021/V47/I15/77
[1] AYTAC BAL F,OZKOCAK I,CADIRCI B H,et al.Effects of photodynamic therapy with indocyanine green on Streptococcus mutans biofilm[J].Photodiagnosis and Photodynamic Therapy,2019,26:229-234.
[2] KASSEBAUM N J,BERNABÉ E,DAHIYA M,et al.Global burden of untreated caries:A systematic review and metaregression[J].Journal of Dental Research,2015,94(5):650-658.
[3] DU M Q,LI Z,JIANG H,et al.Dental caries status and its associated factors among 3-to 5-year-old children in china:A national survey[J].Chinese Journal of Dental Research,2018,21(3):167-179.
[4] XIAO J,KLEIN M I,FALSETTA M L,et al.The exopolysaccharide matrix modulates the interaction between 3D architecture and virulence of a mixed-species oral biofilm[J].PLoS Pathogens,2012,8(4):e1002623.
[5] YUE J X,YANG H Y,LIU S Y,et al.Influence of naringenin on the biofilm formation of Streptococcus mutans[J].Journal of Dentistry,2018,76:24-31.
[6] HE J Z,WANG S D,WU T X,et al.Effects of ginkgoneolic acid on the growth,acidogenicity,adherence,and biofilm of Streptococcus mutans in vitro[J].Folia Microbiologica,2013,58(2):147-153.
[7] KULSHRESTHA S,KHAN S,HASAN S,et al.Calcium fluoride nanoparticles induced suppression of Streptococcus mutans biofilm:An in vitro and in vivo approach[J].Applied Microbiology and Biotechnology,2016,100(4):1 901-1 914.
[8] KOO H,DUARTE S,MURATA R M,et al.Influence of cranberry proanthocyanidins on formation of biofilms by Streptococcus mutans on saliva-coated apatitic surface and on dental caries development in vivo[J].Caries Research,2010,44(2):116-126.
[9] KIM D,HWANG G,LIU Y,et al.Cranberry flavonoids modulate cariogenic properties of mixed-species biofilm through exopolysaccharides-matrix disruption[J].PLoS One,2015,10(12).DOI:10.1371/journal.pone.0145844.
[10] ANDRÉ C B,ROSALEN P L,GALVÃO L C D C,et al.Modulation of Streptococcus mutans virulence by dental adhesives containing anti-caries agents[J].Dental Materials,2017,33(10):1 084-1 092.
[11] NATTO Z S,HAMEEDALDAIN A.Methodological quality assessment of meta-analyses and systematic reviews of the relationship between periodontal and systemic diseases[J].Journal of Evidence Based Dental Practice,2019,19(2):131-139.
[12] JAKUBOVICS N S.Oral microbiology.Encyclopedia of Microbiology (Fourth Edition)[M].Oxford:Academic Press.2019.
[13] TEANPAISAN R,PIWAT S,TIANVIWAT S,et al.Effect of long-term consumption of Lactobacillus paracasei SD1 on reducing mutans streptococci and caries risk:A randomized placebo-controlled trial[J].Dentistry Journal,2015,3(2):43-54.
[14] SIDHU G K,MANTHA S,MURTHI S,et al.Evaluation of Lactobacillus and Streptococcus mutans by addition of probiotics in the form of curd in the diet[J].Journal of International Oral Health,2015,7(7):85-89.
[15] FERNANDES T,BHAVSAR C,SAWARKAR S,et al.Current and novel approaches for control of dental biofilm[J].International Journal of Pharmaceutics,2018,536(1):199-210.
[16] COMPARE D,ROCCO A,COCCOLI P,et al.Lactobacillus casei DG and its postbiotic reduce the inflammatory mucosal response:An ex-vivo organ culture model of post-infectious irritable bowel syndrome[J].BMC Gastroenterology,2017,17(1):53
[17] 姚沛琳. 乳酸菌抑制变异链球菌生物膜形成的研究[D].无锡:江南大学,2015.YAO P L.Inhibition of lactic acid bacteria on the biofilm formation of Streptococcus mutans[D].Wuxi:Jiangnan University,2015.
[18] ZHANG G,LU M,LIU R M,et al.Inhibition of Streptococcus mutans biofilm formation and virulence by Lactobacillus plantarum K41 isolated from traditional Sichuan pickles[J].Frontiers in Microbiology,2020,11:774.
[19] WU C C,LIN C T,WU C Y,et al.Inhibitory effect of Lactobacillus salivarius on Streptococcus mutans biofilm formation[J].Molecular Oral Microbiology,2015,30(1):16-26.
[20] SAMOT J,LEBRETON J,BADET C.Adherence capacities of oral Lactobacilli for potential probiotic purposes[J].Anaerobe,2011,17(2):69-72.
[21] KHAN R,ZAKIR M,KHANAM Z,et al.Novel compound from Trachyspermum ammi (Ajowan caraway) seeds with antibiofilm and antiadherence activities against Streptococcus mutans:A potential chemotherapeutic agent against dental caries[J].Journal of Applied Microbiology,2010,109(6):2 151-2 159.
[22] WASFI R,MANSOUR L E,HASHEM A M,et al.Antimicrobial activities against biofilm formed by Proteus mirabilis isolates from wound and urinary tract infections[J].Indian Journal of Medical Microbiology,2012,30(1):76-80.
[23] RIBEIRO S C,O’CONNOR P M,ROSS R P,et al.An anti-listerial Lactococcus lactis strain isolated from Azorean Pico cheese produces lacticin 481[J].International Dairy Journal,2016,63:18-28.
[24] MATSUMOTO-NAKANO M,NAGAYAMA K,KITAGORI H,et al.Inhibitory effects of Oenothera biennis (evening primrose) seed extract on Streptococcus mutans and S.mutans-induced dental caries in rats[J].Caries Research,2011,45(1):56-63.
[25] 徐显睿, 李翠凤,杨洪来,等.具有益生潜力的乳酸杆菌筛选[J].乳业科学与技术,2020,43(2):8-12.XU X R,LI C F,YANG H L,et al.Screening of Lactobacillus for probiotic potential[J].Journal of Dairy Science and Technology,2020,43(2):8-12.
[26] PERES C M,ALVES M,HERNANDEZ-MENDOZA A,et al.Novel isolates of Lactobacilli from fermented Portuguese olive as potential probiotics[J].LWT-Food Science and Technology,2014,59(1):234-246.
[27] 薛龙, 王立鹤,聂红兵,等.唾液乳酸杆菌w22a和种植体周围炎致病菌体外作用的初步研究[J].口腔医学研究,2016,32(10):1 042-1 046.XUE L,WANG L H,NIE H B,et al.Antibacterial effects of Lactobacillus salivarius W22a on the periodontal pathogens[J].Journal of Oral Science Research,2016,32(10):1 042-1 046.
[28] 张秋香, 黄银,姚沛琳,等.植物乳杆菌FB-T9抑制变异链球菌及其生物膜形成的研究[J].食品与生物技术学报,2019,38(9):17-26.ZHANG Q X,HUANG Y,YAO P L,et al.Inhibition of Streptococcus mutans and its biofilm formation by Lactobacillus plantarum FB-T9[J].Journal of Food Science and Biotechnology,2019,38(9):17-26.
[29] SULAIMAN N D,NIKEN A,NUR I J,et al.Isolation and partial purification of lysozyme from saliva of Bali cattle (Bos sondaicus) using an aqueous mixture of polyethylene glycol (PEG) with sodium sulfate[J].African Journal of Biotechnology,2012,11(8):1 977-1 980.
[30] TORLAKOVIC L,KLEPAC-CERAJ V,ØGAARD B,et al.Microbial community succession on developing lesions on human enamel[J].Journal of Oral Microbiology,2012,4(1):16 125.
[1] 马申嫣, 王晶, 赵岩, 曹江, 翟齐啸, 张灏, 赵建新, 田丰伟, 陈卫. 以巧克力为载体的益生菌膳食补充剂的开发[J]. 食品与发酵工业, 2021, 47(9): 143-148.
[2] 张恕铭, 曾林, 孙向阳, 汪杰, 孙擎, 张庆, 谭霄. 屎肠球菌与植物乳杆菌共培养产γ-氨基丁酸条件优化及关键酶活性研究[J]. 食品与发酵工业, 2021, 47(9): 154-159.
[3] 胡丽丽, 董庆利, 夏阳, 张帅帅, 杨静远, 王真, 刘阳泰. 单增李斯特菌生物膜形成及其调控机制研究进展[J]. 食品与发酵工业, 2021, 47(8): 276-282.
[4] 孙媛媛, 崔树茂, 唐鑫, 毛丙永, 赵建新, 陈卫. 发酵乳杆菌的生长限制性因素分析及高密度培养工艺优化[J]. 食品与发酵工业, 2021, 47(6): 1-10.
[5] 彭明芳, 李培骏, 单杨, 陈玉秋, 杨岱峻, 雷丽嫦, 黄芝辉, 余孔新. 比较基因组揭示广西酸菜乳杆菌碳水化合物活性酶谱[J]. 食品与发酵工业, 2021, 47(4): 68-73.
[6] 徐晚晴, 张秋香, 郑彦懿, 赵建新, 马方励. 治疗牙周炎的乳杆菌筛选及口腔益生特性评价[J]. 食品与发酵工业, 2021, 47(15): 70-76.
[7] 聂彩清, 艾连中, 熊智强, 张汇. 高效阴离子交换色谱-脉冲安培法分析嗜热链球菌胞外多糖的单糖组成[J]. 食品与发酵工业, 2021, 47(15): 255-261.
[8] 吴静, 李伟, 冯静静, 周梦洁, 胡汶松, 汪剑, SOKHNA MBACKE Gningue, 吴佳雯, 赵礼军, 徐文瀚, 薛正莲, 王洲, 刘艳. 不同培养条件对枯草芽胞杆菌BS168-ΔsinR生物膜形成及维生素K2产量的影响[J]. 食品与发酵工业, 2021, 47(14): 23-30.
[9] 丹彤, 田佳乐, 乔少婷. 具有良好风味德氏乳杆菌保加利亚亚种的筛选及其产香性能分析[J]. 食品与发酵工业, 2021, 47(14): 229-234.
[10] 王东旭, 尹成男, 叶华, 郭元新. 热灭活鼠李糖乳杆菌HN001对DSS诱导的小鼠结肠炎保护作用[J]. 食品与发酵工业, 2021, 47(13): 30-35.
[11] 孙盛, 陈作国, 俞赟霞, 曲冬梅, 余腾斐, 李言郡, 陈苏. 产胞外多糖植物乳杆菌WHH589的免疫活性及其在发酵乳中的应用[J]. 食品与发酵工业, 2021, 47(13): 43-50.
[12] 马申嫣, 王晶, 朱家民, 曹江, 翟齐啸, 张灏, 赵建新, 田丰伟, 陈卫. 植物蛋白-益生菌复合制剂对小鼠铅毒性的缓解作用[J]. 食品与发酵工业, 2021, 47(13): 51-58.
[13] 肖健, 蔡国林, 吴殿辉, 李晓敏, 陆健. 酸啤酒酿造用乳酸菌的筛选及应用[J]. 食品与发酵工业, 2021, 47(13): 107-111.
[14] 石阳阳, 江远智, 李瑞, 严利文, 赵建新, 陈卫, 张灏, 杭锋. 协同发酵生产植物乳杆菌发酵乳及其特性研究[J]. 食品与发酵工业, 2021, 47(11): 8-13.
[15] 陈大卫, 梁娇娇, 程月, 瞿恒贤, 陈春萌, 任晨瑜, 张臣臣, 关成冉, 马文龙, 陈霞, 李启明, 顾瑞霞. 鼠李糖乳杆菌hsryfm 1301发酵乳在非酒精性脂肪肝细胞模型中对人肝细胞L-02的益生作用[J]. 食品与发酵工业, 2021, 47(11): 61-67.
[1] ZHANG Dong et al. Effect of different amounts of salt on quality of bacon[J]. Food and Fermentation Industries, 2017, 43(11): 159 .
[2] . Effect of Protein on Quality of Chinese Rice Wine #br# [J]. Food and Fermentation Industries, 0, (): 1 .
[3] . Construction of Fast Purification Method of Recombinant Alkaline Protease with FPLC[J]. Food and Fermentation Industries, 2002, 28(4): 11 .
[4] . [J]. Food and Fermentation Industries, 2002, 28(4): 74 .
[5] . The Study on High Activity Xylanase Production from Streptomyces olivaceovirdis E-86 by Different Inducer[J]. Food and Fermentation Industries, 2002, 28(6): 23 .
[6] . [J]. Food and Fermentation Industries, 2001, 27(8): 40 .
[7] Ge Hanjing,Chen Shanshan,Qiu Nongxue. Determining the Main Factors of Granny Smith for Apple Juice Processing[J]. Food and Fermentation Industries, 2005, 31(11): 130 .
[8] . [J]. Food and Fermentation Industries, 2006, 32(2): 136 .
[9] Chen Hongying,Wang Zheng,Pan Shuailu,Ying Hong,Zhang Suyi,Hu Cheng. Primary Study on Xylitol Production of Wine Lees Hydrolysate by Microencapsulated Candida tropicalis Cells[J]. Food and Fermentation Industries, 2006, 32(3): 40 .
[10] Chen Lin,Wu Qing,Wei Wei,Tang Limin. Study on the Antioxidant Activity of the Extract of the Leaves of Lagerstroemia specious L.[J]. Food and Fermentation Industries, 2006, 32(3): 47 .
Viewed
Full text


Abstract

Cited

  Shared   
  Discussed   
版权所有 © 《食品与发酵工业》编辑部
地址:北京朝阳区酒仙桥中路24号院6号楼111室
本系统由北京玛格泰克科技发展有限公司设计开发  技术支持:support@magtech.com.cn