Abstract: Four Lactobacillus strains were evaluated in vitro by demonstrate high hydrophobicity, electrostatic charge, tolerance to lysozyme and lower biofilm formation ability. The auto-aggregation ability of four tested Lacticaseibacillus rhamnosus B6, Limosilactobacillusfermentum B44, Lacticaseibacillus casei LC2W and Lactiplantiacillus plantarum ST-III were 37.67%-60.89%, while their co-aggregating with Streptococcus mutans, the predominant pathogen causing dental caries in oral cavity,reached 63.47%, 46.42%, 60.5% and 61.51%, respectively. When cocultured with S. mutans in BHI containing 3 g/L sucrose, they could reduce the formation of S. mutans biofilm by 31.04%-54.28%, which might be attributed to synergetic effect of organic acids as well as other antagonistic substances produced by Lactobacilli. In conclusion, four tested strains have potentials as oral probiotics candidates in preventing dental caries.
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