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食品与发酵工业  2021, Vol. 47 Issue (13): 66-71    DOI: 10.13995/j.cnki.11-1802/ts.026456
  研究报告 本期目录 | 过刊浏览 | 高级检索 |
牡蛎蛋白酶解产物及其超滤组分抗皮肤光老化活性研究
彭志兰1, 冯畅1, 陈贝贝1, 曹文红1,2, 高加龙1,2, 秦小明1,2, 章超桦1,2*
1(广东海洋大学 食品科技学院,广东省水产品加工与安全重点实验室,广东 湛江,524008)
2(国家贝类加工技术研发分中心(湛江),广东 湛江,524008)
Anti-skin photoaging effect of enzymatic hydrolysate and their ultrafiltration fractions of oyster protein
PENG Zhilan1, FENG Chang1, CHEN Beibei1, CAO Wenhong1,2, GAO Jialong1,2, QIN Xiaoming1,2, ZHANG Chaohua1,2*
1(College of Food Science and Technology,Guangdong Ocean University,Guangdong Provincial Key Laboratory of Aquatic Products Processing and Safety,Zhanjiang 524088,China)
2(National Research and Development Branch Center for Shellfish Processing (Zhanjiang),Zhanjiang 524088,China)
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摘要 以香港牡蛎蛋白为原料,采用中性蛋白酶进行酶解制备牡蛎蛋白酶解产物(oyster protein enzymatic hydrolysate,OPEH),逐级通过8 k、5 k、3 kDa分子筛超滤膜,共截留出<3 kDa(OPEH-1)、3 k ~5 kDa(OPEH-2)、5 k~8 kDa(OPEH-3)和>8 kDa(OPEH-4)等超滤组分。首先对比OPEH及4个超滤组分的体外总抗氧化能力,然后在中波紫外线(ultraviolet B,UVB)诱导的HaCaT(人永生化角层细胞系)光老化细胞模型上,评价其抗皮肤光老化活性,对可能机制进行初步探索。体外总抗氧化能力结果显示,OPEH-1的ABTS阳离子自由基半清除质量浓度(IC50)最低为156.083 μg/mL,具有最佳的体外抗氧化能力。细胞实验结果显示,与OPEH以及其他超滤组分相比,OPEH-1在质量浓度>50 μg/mL 后就能显著地提升UVB辐照后HaCaT细胞的活力,能逆转UVB辐射后的HaCaT细胞形态改变,抑制细胞内活性氧的产生,促进I型胶原蛋白前体的分泌。研究发现,OPEH-1在UVB辐射诱导的HaCaT光老化细胞模型上,具有最好的抗光老化活性,这种活性可能与OPEH-1具有较好的抗氧化能力以及促进I胶原蛋白前体的表达相关。
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彭志兰
冯畅
陈贝贝
曹文红
高加龙
秦小明
章超桦
关键词:  牡蛎  酶解产物  皮肤光老化  抗氧化  I型胶原蛋白前体    
Abstract: Hongkong oyster protein was hydrolyzed by neutral protease firstly. Subsequently, enzymatic oyster protein hydrolysate (OPEH) was gradually ultra-filtrated by 8, 5 and 3 kDa membrane with molecular weight cutoffs to separate four fractions, including<3 kDa (OPEH-1), 3-5 kDa (OPEH-2), 5-8 kDa (OPEH-3), and >8kDa (OPEH-3) fractions. Finally, the total antioxidant capacity of OPEH and four fractions were measured in vitro, and the anti-skin photoaging activities were tested in an UVB-induced HaCaT photoaged cell model. The results of total antioxidant capacity showed that OPEH-1 had the lowest ABTS cation free radical semi-scavenging concentration (IC50), exerted the best antioxidant effect in vitro. The results from cellular experiment indicated that compared with OPEH and other fractions. OPEH-1 significantly improved cell viability starting the concentration in 50 μg/mL, reversed morphological changes of UVB-irradiated HaCaT cells, inhibited the production of intracellular reactive oxygen ROS, while increased the production of extracellular pro-collagen I. The anti-skin photoaging effect of OPEH-1 was possibly associated with its anti-antioxidant property and the activity of improving extracellular pro-collagen I expression.
Key words:  oyster    enzymatic hydrolysate    skin photoaging    antioxidation    pro-collagen I
收稿日期:  2020-12-23      修回日期:  2021-01-29           出版日期:  2021-07-15      发布日期:  2021-08-02      期的出版日期:  2021-07-15
基金资助: 海洋贝类营养健康食品关键技术及产业化项目(GDOU2017052606)
作者简介:  博士研究生(章超桦教授为通讯作者,E-mail:Zhangch2@139.com)
引用本文:    
彭志兰,冯畅,陈贝贝,等. 牡蛎蛋白酶解产物及其超滤组分抗皮肤光老化活性研究[J]. 食品与发酵工业, 2021, 47(13): 66-71.
PENG Zhilan,FENG Chang,CHEN Beibei,et al. Anti-skin photoaging effect of enzymatic hydrolysate and their ultrafiltration fractions of oyster protein[J]. Food and Fermentation Industries, 2021, 47(13): 66-71.
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