1(College of Food Science and Technology,Guangdong Ocean University,Guangdong Provincial Key Laboratory of Aquatic Products Processing and Safety,Zhanjiang 524088,China) 2(National Research and Development Branch Center for Shellfish Processing (Zhanjiang),Zhanjiang 524088,China)
Abstract: Hongkong oyster protein was hydrolyzed by neutral protease firstly. Subsequently, enzymatic oyster protein hydrolysate (OPEH) was gradually ultra-filtrated by 8, 5 and 3 kDa membrane with molecular weight cutoffs to separate four fractions, including<3 kDa (OPEH-1), 3-5 kDa (OPEH-2), 5-8 kDa (OPEH-3), and >8kDa (OPEH-3) fractions. Finally, the total antioxidant capacity of OPEH and four fractions were measured in vitro, and the anti-skin photoaging activities were tested in an UVB-induced HaCaT photoaged cell model. The results of total antioxidant capacity showed that OPEH-1 had the lowest ABTS cation free radical semi-scavenging concentration (IC50), exerted the best antioxidant effect in vitro. The results from cellular experiment indicated that compared with OPEH and other fractions. OPEH-1 significantly improved cell viability starting the concentration in 50 μg/mL, reversed morphological changes of UVB-irradiated HaCaT cells, inhibited the production of intracellular reactive oxygen ROS, while increased the production of extracellular pro-collagen I. The anti-skin photoaging effect of OPEH-1 was possibly associated with its anti-antioxidant property and the activity of improving extracellular pro-collagen I expression.
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2021, Vol. 47 
