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食品与发酵工业  2021, Vol. 47 Issue (12): 95-101    DOI: 10.13995/j.cnki.11-1802/ts.026495
  研究报告 本期目录 | 过刊浏览 | 高级检索 |
香辛料对低钠盐肌原纤维蛋白氧化体系凝胶特性的影响
魏娜1, 张建华1, 夏杨毅1,2*
1(西南大学 食品科学学院,重庆,400715)
2(重庆市特色食品工程技术研究中心,重庆,400715)
The effect of spices on the gel properties of low-sodium myofibrillar protein in oxidation system
WEI Na1, ZHANG Jianhua1, XIA Yangyi1,2*
1(College of Food Science, Southwest University, Chongqing 400715, China)
2(Chongqing Special Food Programme and Technology Research Center, Chongqing 400715, China)
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摘要 以肌原纤维蛋白(myofibrillar protein,MP)为原料,添加2.5%(质量分数)的低钠盐加速氧化体系作对照组,探究添加量为0.03%(质量分数)的4种香辛料(丁香、花椒、迷迭香和肉豆蔻)对其凝胶特性的影响。结果表明,氧化周期(72 h)内,添加4种香辛料的低钠盐MP凝胶特性显著降低(P<0.05),与对照组变化趋势一致。同一氧化时间,添加4种香辛料能提高低钠盐MP的凝胶强度、保水性和弹性模量,降低蒸煮损失率,提高氢键、二硫键和离子键含量,降低疏水相互作用含量,而疏水相互作用、二硫键和氢键是维持低钠盐MP凝胶的主要化学作用力,离子键相互作用较小。4种香辛料的添加能抑制低钠盐MP氧化,从而对低钠盐MP凝胶特性有明显的改善作用,有利于优质MP凝胶的形成,且凝胶形成能力按照以下顺序:迷迭香>丁香>肉豆蔻>花椒添加组。这为香辛料在低钠盐MP凝胶中的应用提供理论依据。
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魏娜
张建华
夏杨毅
关键词:  香辛料  肌原纤维蛋白  低钠盐  凝胶特性  蛋白质氧化    
Abstract: The aim of this study was to explore the effects of four spices (clove, pepper, rosemary and nutmeg) of 0.03% mass fraction on the gel properties of low-sodium myofibril protein. Myofibril protein (MP) was used as the raw material and 2.5% low sodium salt accelerated oxidation system was used as control. The results showed that, within the oxidation period (72 h), the gel properties of the low-sodium MP with the addition of four spices were reduced significantly(P<0.05), which was consistent with the changing trend of the control. At the same oxidation time, the addition of four spices could increase the gel strength, water retention and elastic modulus of low-sodium MP. And it could also reduce cooking loss, increase hydrogen bond, disulfide bond and ionic bond content, and reduce hydrophobic interaction content. Furthermore, hydrophobic interactions, disulfide bonds and hydrogen bonds were the main chemical forces that maintain low-sodium MP gel, while ionic bond interactions were small. However, the addition of four spices could significantly inhibit the oxidation of low-sodium MP, thereby improved the properties of low-sodium MP gel significantly which was conducive to the formation of high-quality MP gel. The gel-forming ability was in the following order: rosemary> cloves> nutmeg> pepper adding group. This provides a theoretical basis for the application of spices in the low-sodium MP gel.
Key words:  spice    myofibrillar protein    low sodium salt    gel properties    protein oxidation
收稿日期:  2020-12-23      修回日期:  2021-01-19           出版日期:  2021-06-25      发布日期:  2021-07-22      期的出版日期:  2021-06-25
基金资助: 公益性行业(农业部)科研专项(201303082-7);四川省科技支撑计划项目(2016NZ0003-05)
作者简介:  硕士研究生(夏杨毅副教授为通讯作者,E-mail:2658355128@qq.com)
引用本文:    
魏娜,张建华,夏杨毅. 香辛料对低钠盐肌原纤维蛋白氧化体系凝胶特性的影响[J]. 食品与发酵工业, 2021, 47(12): 95-101.
WEI Na,ZHANG Jianhua,XIA Yangyi. The effect of spices on the gel properties of low-sodium myofibrillar protein in oxidation system[J]. Food and Fermentation Industries, 2021, 47(12): 95-101.
链接本文:  
http://sf1970.cnif.cn/CN/10.13995/j.cnki.11-1802/ts.026495  或          http://sf1970.cnif.cn/CN/Y2021/V47/I12/95
[1] GUO X Y, PENG Z Q, ZHANG Y W, et al.The solubility and conformational characteristics of porcine myosin as affected by the presence of L-lysine and L-histidine[J].Food Chemistry, 2015, 170(6):212-217.
[2] 张建华, 夏杨毅.氧化条件下 HL-低钠盐对鹅肉肌原纤维蛋白凝胶特性的影响[J].食品与发酵工业, 2020, 46(10):166-172.ZHANG J H, XIA Y Y.Effect of HL-low sodium salt on the gel properties of goose meat myofibrillar protein under oxidative conditions[J].Food and Fermentation Industries, 2020, 46(10):166-172.
[3] 马志方. 低钠传统金华火腿加工过程中脂质和蛋白质水解及氧化的研究[D].南京:南京农业大学, 2016.MA Z F.Study on the hydrolysis and oxidation of lipide and protein in low sodium traditional jinhua ham during processing[D].Nanjing:Nanjing Agricultural University, 2016.
[4] LORENZO J M, VARGAS F C, STROZZI I, et al.Influence of pitanga leaf extracts on lipid and protein oxidation of pork burger during shelf-life[J].Food Research International, 2018, 114(12):47-54.
[5] CHEN H, DIAO J, LI Y, et al.The effectiveness of clove extracts in the inhibition of hydroxyl radical oxidation-induced structural and rheological changes in porcine myofibrillar protein[J].Meat Science, 2015,111(7):60-66.
[6] JONGBERG S, TERKELSEN L S, MIKLOS R, et al.Green tea extract impairs meat emulsion properties by disturbing protein disulfide cross-linking[J].Meat Science, 2015, 100(2):2-9.
[7] 李云龙, 赵月亮, 范大明, 等.香辛料中植物化合物对肉制品品质及健康功效影响研究进展[J/OL].食品科学,2021.http://kns.cnki.net/kcms/detail/11.2206.TS.20200722.1522.190.html.LI Y L, ZHAO Y L, FAN D M, et al.Effects of phytochemicals from spices on quality attributes and health benefits of meat products:A review[J/OL].Food Science, 2021.http://kns.cnki.net/kcms/detail/11.2206.TS.20200722.1522.190.html.
[8] 涂宗财, 傅志丰, 王辉, 等.红薯叶不同溶剂提取物抗氧化性及活性成分鉴定[J].食品科学, 2015, 36(17):1-6.TU Z C, FU Z F, WANG H, et al.Comparison of antioxidant activities of various solvent extracts of sweet potato (Ipomoea batatas (L.) Lam.) leaves and identification of antioxidant constituents of the merthanol extract[J].Food Science, 2015, 36(17):1-6.
[9] TAKAI E, YOSHIZAWA S, EJIMA D, et al.Synergistic solubilization of porcine myosin in physiological salt solution by arginine[J].International Journal of Biological Macromolecules, 2013, 62(11):647-651.
[10] 付渊. L-精氨酸、L-赖氨酸对鸡胸肉中肌球蛋白凝胶特性的影响[D].合肥:合肥工业大学, 2016.FU Y.Study on the influences of L-Lys/L-Arg on the gel properties of chicken breast myosin[D].Heifei:Heifei University of Technology, 2016.
[11] 徐幸莲. 兔骨骼肌肌球蛋白热诱导凝胶特性及成胶机制研究[D].南京:南京农业大学, 2003.XU X L.Study on heated-induced gelation properties and gel forming mechanism of myosin from rabbit skeletal muscles[D].Nanjing:Nanjing Agricultural University, 2003.
[12] MA F.Effect of high pressure processing on the gel properties of salt-soluble meat protein containing CaCl2 and k-carrageenan[J].Meat Science, 2013, 95(1):22-26.
[13] GÓMEZ-GUILLÉN M C, BORDERÍAS A J, MONTERO P.Chemical interactions of nonmuscle proteins in the network of sardine(Sardina pilchardus) muscle gels[J].LWT-Food Science and Technology, 1997, 30(6):602-608.
[14] JIA N, WANG L, SHAO J, et al.Changes in the structural and gel properties of pork myofibrillar protein induced by catechin modification[J].Meat Science, 2017, 127(5):45-50.
[15] 王鹏, 穆雅慧, 何思宁, 等.牛骨胶原水解物抑制鲢鱼肌原纤维蛋白氧化和增强凝胶特性研究[J/OL].食品工业科技,2021.https://doi.org/10.13386/j.issn1002-0306.2020080092.WANG P, MU Y H, HE S N, et al.Oxidation inhibition and gel properties enhancement of myofibrillar protein from silver carp by bovine-bone collagen hydrolysate[J/OL].Science and Technology of Food Industry,2021.https://doi.org/10.13386/j.issn1002-0306.2020080092.
[16] LYU Y, FENG X, WANG Y, et al.The gelation properties of myofibrillar proteins prepared with malondialdehyde and (-)-epigallocatechin-3-gallate[J/OL].Food Chemistry, 2020.https://doi.org/10.1016/j.foodchem.2020.127817.
[17] JIANG Y, LI D H, TU J C, et al.Mechanisms of change in gel water-holding capacity of myofibrillar proteins affected by lipid oxidation:The role of protein unfolding and cross-linking[J/OL].Food Chemistry,2020.https://doi.org/10.1016/j.foodchem.2020.128587.
[18] ZHANG Z, YANG Y, TANG X, et al.Chemical forces and waterholding capacity study of heat-induced myofibrillar protein gel as affected by high pressure[J].Food Chemistry, 2015, 188(12):111-118.
[19] 张慧芸, 吴静娟, 郭新宇.多酚对猪肉肌原纤维蛋白氧化和凝胶特性的影响[J].食品科学, 2016, 37(21):43-47.ZHANG H Y, WU J J, GUO X Y.Effect of polyphenols on the oxidation and gel properties of pork myofibrillar protein[J].Food Science, 2016, 37(21):43-47.
[20] RATTAYA S, BENJAKUL S, Prodpran T.Properties of fish skin gelatin film incorporated with seaweed extract[J].Journal of Food Engineering, 2009, 95(1):151-157.
[21] 曹云刚, 马文慧, 艾娜丝, 等.氧化强度对肌原纤维蛋白结构及凝胶性能的影响[J].食品科学, 2019, 40(20):21-27.CAO Y G, MA W H, AI N S, et al.Effects of different oxidation intensities on the structure and gel properties of myofibrillar protein[J].Food Science, 2019, 40(20):21-27.
[22] MONTERO P, GIMÉNEZ B, PÉREZ-MATEOS M, et al.Oxidation stability of muscle with quercetin and rosemary during thermal and high-pressure gelation[J].Food Chemistry, 2005, 93(1):17-23.
[23] 王静宇, 胡新, 刘晓艳, 等.肌原纤维蛋白热诱导凝胶特性及化学作用力研究进展[J].食品与发酵工业, 2020, 46(8):300-306.WANG J Y, HU X, LIU X Y, et al.Advances in heat-induced gel properties and chemical forces of myofibrillar protein gel[J].Food and Fermentation Industries, 2020, 46(8):300-306.
[24] 袁凯, 张龙, 谷东陈, 等.肉品中的蛋白质氧化机制及其影响因素[J].食品科学, 2018, 39(5):329-335.YUAN K, ZHANG L, GU D C, et al.A review of mechanisms and factors affecting protein oxidation in meat[J].Food Science, 2018, 39(5):329-335.
[25] 张自业. 盐和超高压处理对肌原纤维蛋白凝胶特性与作用力的影响及调控机理研究[D].南京:南京财经大学, 2016.ZHANG Z Y.Study on effects of salt and high pressure treament on properties and chemical forces of myofibrillar protein gel and the regulation mechanism[D].Nanjing:Nanjing University of Finance and Economics, 2016.
[26] 陈雪珂. 肌球蛋白凝胶化过程中与迷迭香酸的互作机制及其对蛋白理化特性的影响[D].重庆:西南大学, 2019.CHEN X K.Interaction mechanism of myosin with rosmarinic acid during gelation and its effect on protein physicochemical properties[D].Chongqing:Southwest University, 2019.
[27] THOMAS J A, MALLIS R J.Aging and oxidation of reactive protein sulphydryls[J].Experimental Gerontology, 2001, 36 (9):1 519-1 526.
[28] 刘丹, 贾娜, 杨磊, 等.3种不同香辛料提取物对猪肉肌原纤维蛋白功能特性的影响[J].食品科学, 2017, 38(15):14-19.LIU D, JIA N, YANG L, et al.Influence of three different spice extracts on functional characteristics of pork myofibrillar protein[J].Food Science, 2017, 38(15):14-19.
[29] 贾娜, 张风雪, 孙嘉, 等.亚麻籽胶对儿茶素-肌原纤维蛋白热诱导凝胶特性的影响[J].食品科学, 2019, 40(20):1-7.JIA N, ZHANG F X, SUN J, et al.Effect of flaxseed gum on heat-induced gel properties of catechins-myofibrillar protein[J].Food Science, 2019, 40(20):1-7.
[30] YAN M Y, LI B F, ZHAO X, et al.Physicochemical properties of gelatin gels from walleye pollock (Theragra chalcogramma) skin cross-linked by gallic acid and rutin[J].Food Hydrocolloids, 2011, 25(5):907-914.
[31] SENGUPTA P, SARDAR P S, ROY P, et al.Investigation on the interaction of Rutin with serum albumins:Insights from spectroscopic and molecular docking techniques[J].Journal of Photochemistry and Photobiology B:Biology, 2018, 183:101-110.
[32] SAITO H, TAGUCHI T, AOKI H, et al.pH-Responsive swelling behavior of collagen gels prepared by novel crosslinkers based on naturally derived di-or tricarboxylic acids[J].Acta Biomaterialia, 2007, 3(1):89-94.
[33] 贾娜, 谢振峰, 李儒仁, 等.迷迭香提取物与NaCl协同改善肌原纤维蛋白凝胶特性[J].食品科学, 2018, 39(3):28-33.JIA N, XIE Z F, LI R R, et al.Synergistic improvement of myofibrillar protein gel properties by combination of rosemary extract and NaCl[J].Food Science, 2018, 39(3):28-33.
[34] TANG C, ZHANG W, ZOU Y, et al.Influence of RosA-protein adducts formation on myofibrillar protein gelation properties under oxidative stress[J].Food Hydrocolloids, 2017, 67(6):197-205.
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[2] .YANG Qian et al . Mutation and functional properties of the histidine residues existed in the conserved regions of Rhizopus oryzae α-amylase[J]. Food and Fermentation Industries, 2017, 43(11): 22 -29 .
[3] LI Xiao-min et a. The influence of feruloyl esterase on filterability of wort[J]. Food and Fermentation Industries, 2017, 43(11): 30 -33 .
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[5] LIU Wen-ying et al. Protective effect of wheat oligopeptides and glutamine against gastrointestinal mucosa damage in rats[J]. Food and Fermentation Industries, 2017, 43(11): 51 -57 .
[6] YAO Hang-hang et al. Composition and physicochemical properties of acid soluble collagen of skin of Yunnan bream[J]. Food and Fermentation Industries, 2017, 43(11): 81 .
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[8] ZHAO Xiang-ying et al. Effect of Glucose on xylitol fermentation byCandida tropicalis SFX - Y9[J]. Food and Fermentation Industries, 2017, 43(11): 107 .
[9] ZHANG Zhe-yuan.et al. Effects of different total solids of goat milk on quality of goat milk yogurt #br# [J]. Food and Fermentation Industries, 2017, 43(11): 112 .
[10] ZHANG Dong et al. Effect of different amounts of salt on quality of bacon[J]. Food and Fermentation Industries, 2017, 43(11): 159 .
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