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食品与发酵工业  2021, Vol. 47 Issue (15): 171-177    DOI: 10.13995/j.cnki.11-1802/ts.026501
  生产与科研应用 本期目录 | 过刊浏览 | 高级检索 |
微波提取对百合多糖流变性及生物活性的影响
黄群惠1,2, 黄志芸1,2, 田文静1,2, 李正逗1,2, 丁玲1,2, 史金涵1,2, 马旺军1,2, 麻玉婷1,2, 张继1,2,3*
1(西北师范大学 生命科学学院,甘肃 兰州,730070)
2(甘肃省特色植物有效成分制品工程技术研究中心,甘肃 兰州,730070)
3(西北师范大学 新农村发展研究院,甘肃 兰州,730070)
Effect of microwave extraction on rheology and biological activity of lily polysaccharide
HUANG Qunhui1,2, HUANG Zhiyun1,2, TIAN Wenjing1,2, LI Zhengdou1,2, DING Ling1,2, SHI Jinhan1,2, MA Wangjun1,2, MA Yuting1,2, ZHANG Ji1,2,3*
1(College of Life Science,Northwest Normal University,Lanzhou 730070,China)
2(Bioactive Products Engineering and Distinctive Plants Technology Research Center for Gansu,Lanzhou 730070,China)
3(Institute of New Rural Development,Northwest Normal University,Lanzhou 730070,China)
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摘要 分别采用不同功率、时间和温度的微波辅助热水浸提法提取百合多糖,并用红外光谱对百合多糖进行初步结构表征,研究百合多糖的流变特性,测试其流动性、触变性。对比了7种不同微波辅助法提取出的百合多糖的体外抗氧化活性。红外光谱分析结果表明,不同微波条件提取出的百合多糖在3 382、2 928、1 636、1 415和802 cm-1等处均有吸收峰,表明百合多糖是酸性多糖;流变性质测定结果显示不同微波条件下提取的百合多糖都具有不同程度的流动性和触变性,可应用于食品加工;体外抗氧化实验显示不同微波辅助提取的百合多糖均有抗氧化活性,在医药研究与生产中具有重要的应用价值。
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黄群惠
黄志芸
田文静
李正逗
丁玲
史金涵
马旺军
麻玉婷
张继
关键词:  百合多糖  微波提取  流变特性  抗氧化    
Abstract: Different power, time and temperature were optimized in microwave-assisted hot water extraction method to extract LPS. And the preliminary structural of LPS was characterized by FT-IR. To study the rheological properties of LPS, and its fluidity and thixotropy, the antioxidant activities in vitro of LPS extracted by seven different microwave-assisted methods were compared. The results showed that the LPS extracted by FT-IR under different microwave conditions had the characteristic absorption peaks of polysaccharides (at 3 382, 2 928, 1 636, 1 415 and 802 cm-1), which indicating that the LPS were an acidic polysaccharide. The results of rheological properties showed that the LPS extracted with different microwave conditions had different degrees of fluidity and thixotropy. And antioxidant in vitro experiments showed that the LPS extracted with different microwave-assisted had antioxidant activities, it has important application value in medical research and production.
Key words:  LPS    microwave-assisted extraction    rheological properties    anti-oxidant activity
收稿日期:  2020-12-19      修回日期:  2021-02-10           出版日期:  2021-08-15      发布日期:  2021-08-23      期的出版日期:  2021-08-15
基金资助: 国家自然基金项目(51873175)
作者简介:  硕士研究生(张继研究员为通讯作者,E-mail:zhangji@nwnu.edu.cn)
引用本文:    
黄群惠,黄志芸,田文静,等. 微波提取对百合多糖流变性及生物活性的影响[J]. 食品与发酵工业, 2021, 47(15): 171-177.
HUANG Qunhui,HUANG Zhiyun,TIAN Wenjing,et al. Effect of microwave extraction on rheology and biological activity of lily polysaccharide[J]. Food and Fermentation Industries, 2021, 47(15): 171-177.
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http://sf1970.cnif.cn/CN/10.13995/j.cnki.11-1802/ts.026501  或          http://sf1970.cnif.cn/CN/Y2021/V47/I15/171
[1] 宋硕.兰州百合多糖的结构鉴定及其抗氧化活性研究[J].现代食品,2020(16):176-179.SONG S.Study on the structure identification and antioxidant activity of Lanzhou lily polysaccharide[J].Modern Food,2020(16):176-179.
[2] SONG Q Q,XIE J H,YU Y,et al.Biological activities and pharmaceutical applications of polysaccharide from natural resources:A review[J].Carbohydrate Polymers:Scientific and Technological Aspects of Industrially Important Polysaccharides,2018,183:91-101.
[3] LIU J,STEFAN W,XU C L.A review of bioactive plant polysaccharides:biological activities,functionalization,and biomedical applications[J].Bioactive Carbohydrates and Dietary Fibre,2015,5(1):31-61.
[4] XU Y Q,LIU N Y,FU X T,et al.Structural characteristics,biological,rheological and thermal properties of the polysaccharide and the degraded polysaccharide from raspberry fruits[J].International Journal of Biological Macromolecules,2019,132:109-118.
[5] XU X J,XU J,ZHANG Y Y,et al.Rheology of triple helical Lentinan in solution:steady shear viscosity and dynamic oscillatory behavior[J].Food Hydrocolloids,2007,22(5):735-741.
[6] FLORINA D,PAULINA M,MARGARIDA M M,et al.Novel mango bars using gellan gum as gelling agent:rheological and microstructural studies[J].LWT-Food Science and Technology,2015,62(1):576-583.
[7] YAKINDRA P T,RAJU A,STEFAN K,et al.Rheological and microstructural properties of the chia seed polysaccharide[J].International Journal of Biological Macromolecules,2015,81:991-999.
[8] 韩晓霞, 李洁,邱国玉,等.不同产地兰州百合中多糖的含量测定[J].甘肃科技,2020,36(2):55-57.HAN X X,LI J,QIU G Y,et al.Determination of polysaccharides in Lilium davidii var.unicolor Salisb.from different places[J].Gansu Science and Technology,2020,36(2):55-57.
[9] 朱泉. 百合多糖分离纯化、结构鉴定及其生物活性研究[D].南京:南京农业大学,2012.ZHU Q.Isolation,purification,structure determination and biological activities of polysaccharides from bulb of Lily (Lilium lancifolium Thunb)[D].Nanjing:Nanjing Agricultural University,2012.
[10] 刘成梅, 万茵,涂宗财,等.百合多糖脱蛋白方法的研究[J].食品科学,2002,23(1):89-90.LIU C M,WAN Y,TU Z C,et al.Study on methods to remove protein from lily-polysaccharides[J].Food Science,2002,23(1):89-90.
[11] 黄玉龙, 高清雅,全婷,等.不同提取方法对兰州百合多糖结构及抗氧化活性的影响[J].现代食品科技,2018,34(11):126-131;94.HUANG Y L,GAO Q Y,QUAN T,et al.Effects of different extraction methods on the composition and antioxidant activity of the polysaccharides in Lilium davidii var.unicolor Salisb[J].Modern Food Science & Technology,2018,34(11):126-131;94.
[12] WEI Y X,LIN Y B,XIE R,et al.The flow behavior,thixotropy and dynamical viscoelasticity of fenugreek gum[J].Journal of Food Engineering,2015,166:21-28.
[13] 和法涛, 刘光鹏,朱风涛,等.微波超声波组合提取猴头菇多糖工艺优化及其抗氧化活性[J].食品与生物技术学报,2019,38(1):74-82.HE F T,LIU G P,ZHU F T,et al.Optimization of microwave-ultrasonic assisted extraction of polysaccharides from Hericium erinaceus antioxidant activity in vitro[J].Journal of Food Science and Biotechnology,2019,38(1):74-82.
[14] 顾欣, 高涛,刘梦雅,等.梁平柚柚皮多糖的提取工艺优化、结构解析及抗氧化能力研究[J].食品与发酵工业,2021,47(7):138-146.GU X,GAO T,LIU M Y,et al.Optimization of extraction technology,structure analysis and antioxidant activity of polysaccharides from Liangping pomelo peel[J].Food and Fermentation Industries,2021,47(7):138-146.
[15] 王晗, 朱华平,李文钊,等.越橘提取物中花青素分析及其体外抗氧化活性[J].食品工业科技,2019,40(23):60-65.WANG H,ZHU H P,LI W Z,et al.Analysis of anthocyanin in Bilberry extract and its antioxidant activity in vitro[J].Science and Technology of Food Industry,2019,40(23):60-65.
[16] GAO J,LIN L Z,SUN B G,et al.A comparison study on polysaccharides extracted from Laminaria japonica using different methods:Structural characterization and bile acid-binding capacity[J].Food & Function,2017,8(9):3 043-3 052.
[17] SUN Y X,LIU J C,JOHN F K.Purification,composition analysis and antioxidant activity of different polysaccharide conjugates (APPs) from the fruiting bodies of Auricularia polytricha[J].Carbohydrate Polymers,2010,82(2):299-304.
[18] 杨嘉丹, 刘婷婷,张闪闪,等.微波辅助提取银耳多糖工艺优化及其流变、凝胶特性[J].食品科学,2019,40(14):289-295.YANG J D,LIU T T,ZHANG S S,et al.Optimization of microwave-assisted extraction and rheological and gelling properties of polysaccharide from Tremella fuciformis[J].Food Science,2019,40(14):289-295.
[19] YE H,YAN Y H,JABBAR S,et al.Extraction optimization,characterization and antioxidant activity in vitro of polysaccharides from mulberry (Morus alba L.) leaves[J].Carbohydrate Polymers:Scientific and Technological Aspects of Industrially Important Polysaccharides,2015,128:52-62.
[20] 李秀秀, 尚静,杨曦,等.多糖的增稠、胶凝及乳化特性研究进展[J/OL].食品科学:1-12[2020-11-12].http://kns.cnki.net/kcms/detail/11.2206.TS.20200924.1454.046.html.LI X X,SHANG J,YANG X,et al.Rheology of food polysaccharides-thickening,gelling and emulsifying properties[J].Food Science:1-12[2020-11-12].http://kns.cnki.net/kcms/detail/11.2206.TS.20200924.1454.046.html.
[21] LIN L H,SHEN M Y,LIU S C,et al.An acidic heteropolysaccharide from Mesona chinensis:rheological properties,gelling behavior and texture characteristics[J].International Journal of Biological Macromolecules,2018,107:1 591-1 598.
[22] 丁玲, 焦彩珍,陈乐乐,等.驴乳的系统流变学性质研究[J].食品工业科技,2020,41(1):69-72;78.DING L,JIAO C Z,CHEN L L,et al.Study on systematic rheological properties of Donkey milk[J].Science and Technology of Food Industry,2020,41(1):69-72;78.
[23] 江飞凤, 谭晓辉,胡鹏刚,等.超声-微波协同提取柚子皮多糖工艺优化及单糖组成、结构和抗氧化活性分析[J].食品与发酵工业,2012,47(2):196-204.JIANG F F,TAN X H,HU P G,et al.Optimization of ultrasonic-microwave extraction process of pomelo peel polysaccharide and analysis of monosaccharide composition,structure and antioxidant activity[J].Food and Fermentation Industries,2012,47(2):196-204.
[24] 杨燕敏,郑振佳,高琳,等.红枣多糖超声波提取、结构表征及抗氧化活性评价[J].食品与发酵工业,2021,47(5):120-126.YANG Y M,ZHENG Z J,GAO L,et al.Ultrasonic extraction technology,structure characterization and antioxidant activity evaluation of jujube polysaccharide[J].Food and Fermentation Industries,2021,47(5):120-126.
[25] CHEN H X,QU Z S,ZHANG M,et al.Antioxidant activities of different fractions of polysaccharide conjugates from green tea (Camellia sinensis)[J].Food Chemistry,2008,106(2):559-563.
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[1] ZHANG Dong et al. Effect of different amounts of salt on quality of bacon[J]. Food and Fermentation Industries, 2017, 43(11): 159 .
[2] . Isolation and identification of anaerobic bacteria in the process of Maotai-flavor liquor brewing[J]. Food and Fermentation Industries, 0, (): 1 .
[3] Zheng Dan et al.. The inhibiting effect of flavonoid “astilbin” on pancreatic lipase[J]. Food and Fermentation Industries, 0, (): 1 .
[4] . Effect of Protein on Quality of Chinese Rice Wine #br# [J]. Food and Fermentation Industries, 0, (): 1 .
[5] Yang Zhaoxia,Li Mei,Wu Qianjun. Identification of Isomerized - α - acids in Beer by HPLC[J]. Food and Fermentation Industries, 2006, 32(6): 100 .
[6] Zhang Tao,Lu Rongrong,Qian Ping,He Jinfeng,Chen Tianpeng. Extraction and Properties of Hempseed Protein[J]. Food and Fermentation Industries, 2008, 34(8): 173 .
[7] . Study on the Soft Capsules of Kiwifruit Seed Oil[J]. Food and Fermentation Industries, 2003, 29(12): 58 .
[8] . [J]. Food and Fermentation Industries, 2003, 29(12): 110 .
[9] TANG Kun-tian,ZHAO Cai-yun,HAN Xing-lin,YAN Yin-zhuo. Determination of organic acids in liquors using ion chromatography[J]. Food and Fermentation Industries, 2016, 42(7): 202 .
[10] ZHAI Xiao-bo,LI Hong-jun,HE Zhi-fei. Advanced on application and research of oregano essential oil in meat and meat products[J]. Food and Fermentation Industries, 2016, 42(11): 270 .
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