Abstract: Aflatoxin seriously threatens human health and causes serious economic losses and arouses widespread concern in food safety.In order to develop effective biological bacterium agent against aflatoxin contamination,this study used preliminary screened strain WTX1 as the research object,the AFB1 degradation rate was up to 83.5%.Through morphological,physiological and biochemical analysis and the evolution of 16S rRNA system,the bacterial strain was screened as Bacillus subtilis.By UPLC-MS/MS determination of degradation rate of strain component parts,the degradation liquid mass spectrum was analyzed by software.The secondary metabolites produced during the growth process played a major role in the degradation of AFB1.The mechanism of AFB1 degradation by strain WTX1 is to destroy the toxic structure of AFB1,namely,furan ring dihydrogen double bond and the lipid ring structure of vanillin to achieve the detoxification effect.The experiment will further analyze the effects of the degradation products of the strain WTX1 on human and animal metabolism.
罗自生,秦雨,徐艳群,等.黄曲霉毒素的生物合成、代谢和毒性研究进展[J].食品科学,2015,36(3):250-257.LUO Z S,QIN Y,XU Y Q,et al.Recent progress in the biosynthesis,metabolism and toxicity of aflatoxins[J].Food Science,2015,36(3):250-257.
KIM S,LEE H,LEE S,et al.Invited review:Microbe-mediated aflatoxin decontamination of dairy products and feeds[J].Journal of Dairy Science,2017,100(2):871-880.
赵萌,高婧,褚华硕,等.黄曲霉毒素B1的分子致毒机理及其微生物脱毒研究进展[J].食品科学,2019,40(11):235-245.ZHAO M,GAO J,CHU H S,et al.Recent progress in research on toxicity mechanism and microbial detoxification of aflatoxin B1[J].Food Science,2019,40(11):235-245.
乔宏兴,姜亚乐,王永芬,等.黄曲霉毒素的危害及其脱毒方法研究进展[J].动物医学进展,2017,38(1):89-93.QIAO H X,JIANG Y L,WANG Y F,et al.Progress on harm and detoxification methods of aflatoxin[J].Progress in Veterinary Medicine,2017,38(1):89-93.
LIU R J,WANG R Q,LU J,et al.Degradation of AFB1 in aqueous medium by electron beam irradiation:Kinetics,pathway and toxicology[J].Food Control,2016,66:151-157.
MOON Y S,KIM H M,CHUN H S,et al.Organic acids suppress aflatoxin production via lowering expression of aflatoxin biosynthesis-related genes in Aspergillus flavus[J].Food Control,2018,88:207-216.
RASTEGAR H,SHOEIBI S,YAZDANPANAH H,et al.Removal of aflatoxin B1 by roasting with lemon juice and/or citric acid in contaminated pistachio nuts[J].Food Control,2017,71:279-284.
蔡俊,田尔诺,邵帅,等.黄曲霉毒素B1生物脱毒的研究进展[J].微生物学通报,2017,44(3):726-731.CAI J,TIAN E N,SHAO S,et al.Progress in biological detoxification of aflatoxin B1[J].Microbiology China,2017,44(3):726-731.
VERHEECKE C,LIBOZ T,MATHIEU F.Microbial degradation of aflatoxin B1:Current status and future advances[J].International Journal of Food Microbiology,2016,237:1-9.
ADEBO O A,NJOBEH P B,GBASHI S,et al.Review on microbial degradation of aflatoxins[J].Critical Reviews in Food Science and Nutrition,2017,57(15):3 208-3 217.
李俊霞, 梁志宏,关舒,等.黄曲霉毒素B1降解菌株的筛选及鉴定[J].中国农业科学,2008,41(5):1 459-14 63.LI J X,LIANG Z H,GUAN S,et al.Screening and identification of aflatoxin B1 degradation strains[J].Scientia Agricultura Sinica,2008,41(5):1 459-1 463
RAKSHA RAO K,VIPIN A V,HARIPRASAD P,et al.Biological detoxification of aflatoxin B1 by Bacillus licheniformis CFR1[J].Food Control,2017,71:234-241.
XIA X S,ZHANG Y,LI M Y,et al.Isolation and characterization of a Bacillus subtilis strain with aflatoxin B1 biodegradation capability[J].Food Control,2017,75:92-98.
SAMUEL M S,SIVARAMAKRISHNA A,MEHTA A.Degradation and detoxification of aflatoxin B1 by pseudomonas putida[J].International Biodeterioration & Biodegradation,2014,86:202-209.
GARRITY G M,BELL J A,LILBURN T G.Bergey’s manual of systematic bacteriology [M].Ninth Edition.New York:Springer New York Berlin Herdelberg,2004.
东秀珠,蔡妙英.常见细菌系统鉴定手册[M].北京:科学出版社,2001:318-419.DONG X Z,CAI M Y.Manual for systematic identification of common bacteria[M].Beijing:Science Press,2001:318-419.
唐璎,孟宪刚,邓展瑞,等.西北酸菜中吸附黄曲霉毒素B1乳酸菌株的筛选鉴定及稳定性研究[J].食品与发酵工业,2020,46(15):60-65.TANG Y,MENG X G,DENG Z R,et al.Screening and stability of an aflatoxin B1-adsorbing lactic acid bacterium isolated from pickled vegetable in northwest China[J].Food and Fermentation Industries,2020,46(15):60-65.
XU L,AHMED M F E,SANGARE L,et al.Novel aflatoxin-degrading enzyme from Bacillus shackletonii L7[J].Toxins,2017,9(1):36.
GOMAA E Z,ABDELALL M F,ELMAHDY O M.Detoxification of aflatoxin B1 by antifungal compounds from Lactobacillus brevis and Lactobacillus paracasei,isolated from dairy products[J].Probiotics and Antimicrobial Proteins,2018,10(2):201-209.
SHUKLA S,PARK J H,CHUNG S H,et al.Ochratoxin a reduction ability of biocontrol agent Bacillus subtilis isolated from Korean traditional fermented food kimchi[J].Scientific Reports,2018,8(1):8 039.
李文明. 黄曲霉毒素B1生物降解产物的分离鉴定及其致突变性研究[D].南昌:南昌大学,2013.LI W M.Separation and characteristics of the products derived from biodegraded aflatoxin B1 [D].Nanchang:Nanchang University,2013.
孟繁磊,宋志峰,牛红红,等.基于UPLC-MS-MS技术快速测定花生中4种黄曲霉毒素[J].中国油脂,2018,43(1):136-139.MENG F L,SONG Z F,NIU H H,et al.Rapid determination of four kinds of aflatoxins in peanut by UPLC-MS-MS[J].China Oils and Fats,2018,43(1):136-139.
王宁.基于菌藻联合策略防控刺参圈水绵的研究[D].大连:大连理工大学,2019.WANG N.Study on prevention and control of spirogyra in sea cucumber culture lagoon based on co-treatment strategy with mixed bacteria and microalgae[D].Dalian:Dalian University of Technology,2019.
BA C,ZHANG L,ZHANG P,et al.Isolation,identification and characterization of two strains of Bacillus subtilis[J].Journal of South China Agricultural University,2017,38(3):46-51.
ESHELLI M,HARVEY L,EDRADA E R,et al.Metabolomics of the bio-degradation process of aflatoxin B1 by actinomycetes at an initial pH of 6.0[J].Toxins (Basel),2015,7(2):439-456.