Abstract: Phospholipase D (PLD) is a kind of ester hydrolase, which catalyzes biotransformation of lecithin to phosphatidylserine and therefore shows great potential in food and pharmaceutical industries. In this study, a phospholipase D was produced by Bacillus cereus ZY12, and 82 mU/mL of activity was obtained when the strain was cultured under 30 ℃ and pH 7 for 36 h in medium containing 20 g/L of yolk and 2 mmol/L of MgSO4·7H2O. Further investigation showed that the expression of PLD in this strain could be induced by yolk, lecithin from soybean, lecithin from peanut, and lecithin from sunflower, while was completely inhibited by glucose, fructose and maltose.
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