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食品与发酵工业  2021, Vol. 47 Issue (9): 57-62    DOI: 10.13995/j.cnki.11-1802/ts.026520
  研究报告 本期目录 | 过刊浏览 | 高级检索 |
蜡状芽孢杆菌ZY12产磷脂酶D的影响因素
赵雨1, 郭建华1, 张春枝2*
1(齐齐哈尔大学 食品与生物工程学院,黑龙江 齐齐哈尔,161006)
2(大连工业大学 生物工程学院,辽宁 大连,116034)
Factors influencing phospholipase D production by Bacillus cereus ZY12
ZHAO Yu1, GUO Jianhua1, ZHANG Chunzhi2*
1(School of Food and Biological Engineering,Qiqihar University,Qiqihar 161006,China)
2(School of Biological Engineering,Dalian Polytechnic University,Dalian 116034,China)
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摘要 磷脂酶D是一类酯键水解酶,能够生物转化卵磷脂生成磷酯酰丝氨酸,在食品及医药领域有重要应用。为高效获得磷脂酶D,实现生物转化生产磷酯酰丝氨酸,以前期获得的1株具有磷脂酶D活性的蜡状芽孢杆菌ZY12为发酵菌株,通过单因素试验,确定影响蜡状芽孢杆菌产生磷脂酶D的条件因素。结果表明,20 g/L蛋黄、2 mmol/L MgSO4·7H2O、培养温度30 ℃、pH为7、培养时间36 h,磷脂酶D活性最高为82 mU/mL。同时研究发现,除蛋黄外,大豆卵磷脂、花生卵磷脂、葵花卵磷脂、水溶性卵磷脂均可诱导菌株ZY12产生磷脂酶D,葡萄糖、果糖、麦芽糖则会抑制磷脂酶D的产生。
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赵雨
郭建华
张春枝
关键词:  蜡状芽孢杆菌  磷脂酶D  磷酯酰丝氨酸  诱导产酶  发酵条件    
Abstract: Phospholipase D (PLD) is a kind of ester hydrolase, which catalyzes biotransformation of lecithin to phosphatidylserine and therefore shows great potential in food and pharmaceutical industries. In this study, a phospholipase D was produced by Bacillus cereus ZY12, and 82 mU/mL of activity was obtained when the strain was cultured under 30 ℃ and pH 7 for 36 h in medium containing 20 g/L of yolk and 2 mmol/L of MgSO4·7H2O. Further investigation showed that the expression of PLD in this strain could be induced by yolk, lecithin from soybean, lecithin from peanut, and lecithin from sunflower, while was completely inhibited by glucose, fructose and maltose.
Key words:  Bacillus cereus    phospholipase D    phosphatidylserine    induced enzyme    fermentation conditions
收稿日期:  2020-12-24      修回日期:  2021-01-09           出版日期:  2021-05-15      发布日期:  2021-06-03      期的出版日期:  2021-05-15
基金资助: 黑龙江省自然科学基金面上项目(C2017066);2019黑龙江省省属高等学校基本科研业务费科研项目(135409221)
作者简介:  博士,讲师(张春枝教授为通讯作者,E-mail:zhangcz@dlpu.edu.cn)
引用本文:    
赵雨,郭建华,张春枝. 蜡状芽孢杆菌ZY12产磷脂酶D的影响因素[J]. 食品与发酵工业, 2021, 47(9): 57-62.
ZHAO Yu,GUO Jianhua,ZHANG Chunzhi. Factors influencing phospholipase D production by Bacillus cereus ZY12[J]. Food and Fermentation Industries, 2021, 47(9): 57-62.
链接本文:  
http://sf1970.cnif.cn/CN/10.13995/j.cnki.11-1802/ts.026520  或          http://sf1970.cnif.cn/CN/Y2021/V47/I9/57
[1] ZAMANIAN G,PARTOAZAR A,TAVANGAR S M,et al.Effect of phosphatidylserine on cirrhosis-induced hepatic encephalopathy: Response to acute endotoxemia in cirrhotic rats[J].Life Sciences,2020,253.DOI:10.1016/j.lfs.2020.117606.
[2] HUANG L,MA J Y,SANG J C,et al.Enhancing the thermostability of phospholipase D from Streptomyces halstedii by directed evolution and elucidating the mechanism of a key amino acid residue using molecular dynamics simulation[J].International Journal of Biological Macromolecules,2020,164:3 065-3 074.
[3] MAHMOOD Z K,JIN Z A M,JESSE F F,et al.Relationship between the Corynebacterium pseudotuberculosis,phospholipase D inoculation and the fertility characteristics of crossbred Boer Bucks[J].Livestock Science,2016,191:12-21.
[4] JEUCKEN A,HELMS J B,BROUWERS J F.Cardiolipin synthases of Escherichia coli have phospholipid class specific phospholipase D activity dependent on endogenous and foreign phospholipids[J].Biochimica et Biophysica Acta (BBA)-Molecular and Cell Biology of Lipids,2018,1863(10):1 345-1 353.
[5] ZHANG Z X,CHEN M,XU W,et al.Microbial phospholipase D: Identification,modification and application[J].Trends in Food Science & Technology,2020,96:145-156.
[6] YAO Y F,LI J X,LIN Y Y,et al.Structural insights into phospholipase D function[J].Progress in Lipid Research,2021,81:101 070.
[7] ZHAO Y,XU Y F,YU F,et al.Identification of a novel phospholipase D gene and effects of carbon sources on its expression in Bacillus cereus ZY12[J].Journal of Microbiology,2018,56(4):264-271.
[8] MAO X Z,LIU Q Q,QIU Y Q,et al.Identification of a novel phospholipase D with high transphosphatidylation activity and its application in synthesis of phosphatidylserine and DHA-phosphatidylserine[J].Journal of Biotechnology,2017,249:51-58.
[9] MAO S H,ZHANG Z H,MA X Y,et al.Efficient secretion expression of phospholipase D in Bacillus subtilis and its application in synthesis of phosphatidylserine by enzyme immobilization[J].International Journal of Biological Macromolecules,2021,169:282-289.
[10] ZHAO Y,SUI S S,YU F,et al.Improved expression of Bacillus cereus phospholipase D in E.coli [J].Arch Latinoam Nutr,2019,69(4):137-143.
[11] 徐银凤,赵雨,孙迪,等.蜡状芽孢杆菌磷脂酶D的活性形式及HKD活性区域的分子改造对其活性的影响[J].中国生物化学与分子生物学报,2017,33(11):1 161-1 167.
XU Y F,ZHAO Y,SUN D,et al.The active form of phospholipase D from Bacillus cereus and the effect of molecular modification of HKD active region on its activity[J].Chinese Journal of Biochemistry and Molecular Biology,2017,33(11):1 161-1 167.
[12] 张红宇,万嗣宝,尹京苑.低温贮藏中桃果实磷脂酶D活性的测定[J].食品科技,2012,37(5):22-25.
ZHANG H Y,WAN S B,YIN J Y.Improved method for detecting phospholipase D activity of peach fruit during cold storage[J].Food Science and Technology,2012,37(5):22-25.
[13] 王庆玲,金永国,卢士玲,等.不同禽蛋蛋黄甘油三酯的脂质组学比较研究[J].现代食品科技,2017,33(2):210-216.
WANG Q L,JIN Y G,LU S L,et al.Lipidomic analysis of egg yolk triglycerides in different poultry eggs[J].Modern Food Science and Technology,2017,33(2):210-216.
[14] CARREA G,D'ARRIGO P,PIERGIANNI V,et al.Purification and properties of two phospholipases D from Streptomyces sp.[J].Biochimica et Biophysica Acta (BBA)-Lipids and Lipid Metabolism,1995,1 255(3):273-279.
[15] HAGISHITA T,NISHIKAWA M,HATANAKA T.Isolation of phospholipase D producing microorganisms with high transphosphatidylation activity[J].Biotechnology Letters,2000,22(20):1 587-1 590.
[16] 石创,张薇,王一丁.磷脂酶D产生菌株的筛选及其转磷脂化反应特性[J].四川大学学报(自然科学版),2016,53(1):215-220.
SHI C,ZHANG W,WANG Y D.Screening of a phospholipase D producing strain and its transphosphatidylation activity[J].Journal of Sichuan University (Natural Science Edition),2016,53(1):215-220.
[17] 杨学利,张璐,王一丁.高产磷脂酶 D 菌株的筛选、鉴定及发酵条件优化[J].江苏农业科学,2016,44(12):521 -525.
YANG X L,ZHANG L,WANG Y D.Screening,identification and fermentation conditions optimization of high activity phospholipase D strain [J].Jiangsu Agricultural Sciences,2016,44(12):521-525.
[18] HOU H J,GONG J S,DONG Y X,et al.Phospholipase D engineering for improving the biocatalytic synthesis of phosphatidylserine[J].Bioprocess and Biosystems Engineering,2019,42(7):1 185-1 194.
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