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食品与发酵工业  2021, Vol. 47 Issue (21): 46-52    DOI: 10.13995/j.cnki.11-1802/ts.026645
  研究报告 本期目录 | 过刊浏览 | 高级检索 |
胶红酵母Rhodotorula mucilaginosa CM-1菌株的鉴定及胞外多糖的分离纯化
马文锦*, 李梅林, 王博, 周彦兵, 张永显, 于长青, 彭涛
(甘肃省轻工研究院有限责任公司,甘肃 兰州,730000)
Identification of Rhodotorula mucilaginosa CM-1 strain and isolation and purification of extracellular polysaccharide
MA Wenjin*, LI Meilin, WANG Bo, ZHOU Yanbing, ZHANG Yongxian, YU Changqing, PENG Tao
(Gansu Institute of Light Industry Limited Liability Company, Lanzhou 730000, China)
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摘要 通过形态学、碳源/氮源同化实验及18S rDNA生物学方法将实验室筛选得到的菌株CM-1进行鉴定,经鉴定菌株为胶红酵母Rhodotorula mucilaginosa,对R.mucilaginosa CM-1进行无机培养基分离,纯化得到了均一、纯度高的酵母胞外多糖组分RmEPS-11,并对其进行分子质量分布、红外光谱、单糖组成及甲基化分析。结果显示,多糖组分RmEPS-11的平均分子质量为2.3×105Da,其组分是由摩尔比为1.3∶4.4∶7.8∶86.4的阿拉伯糖、半乳糖、葡萄糖和甘露糖组成。RmEPS-11的骨架由1,3-连接的甘露糖和1,2-连接的葡萄糖残基组成,分支上是1,2,6连接的半乳糖和1,2,3,4连接的阿拉伯糖,研究结果为后续胶红酵母胞外多糖的结构解析与活性分析提供了理论和数据支撑。
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马文锦
李梅林
王博
周彦兵
张永显
于长青
彭涛
关键词:  胶红酵母  菌株鉴定  胞外多糖  分离纯化    
Abstract: A strain CM-1 was screened and identified as Rhodotorula mucilaginosa by morphology, carbon/nitrogen source assimilation experiment and 18S rDNA sequence. A homogeneous and highly purified exopolysaccharide (RmEPS-11) was isolated and purified. The molecular weight distribution, infrared spectrum, monosaccharide composition and methylation of RmEPS-11 were analyzed. The results showed that the average molecular weight of RMPS-11 was 2.3×105 Da, and it was composed of arabinose, galactose, glucose and mannose with a molar ratio of 1.3∶4.4∶7.8∶86.4. The skeleton of RMEPS-11 was composed of 1, 3-linked mannose and 1, 2-linked glucose residues, and on the branches were 1,2,6-linked galactose and 1,2,3,4-linked arabinose. The results provide theoretical and data support for the structure analysis and activity analysis of exopolysaccharide from R. mucilaginosa.
Key words:  Rhodotorula mucilaginosa    strain identification    exo-polysaccharide    isolation and purification
收稿日期:  2021-01-04      修回日期:  2021-05-17           出版日期:  2021-11-15      发布日期:  2021-11-30      期的出版日期:  2021-11-15
基金资助: 甘肃省2020年度重点人才项目(2020RCXM078);西北特色农畜产品深加工国家地方联合工程研究中心(发改高技[2015]3173号);甘肃省科技计划项目(18 JR2 JA003);兰州市科技计划项目(2 019-4-41)
作者简介:  博士,高级工程师(通讯作者,E-mail:mawj0707@163.com)
引用本文:    
马文锦,李梅林,王博,等. 胶红酵母Rhodotorula mucilaginosa CM-1菌株的鉴定及胞外多糖的分离纯化[J]. 食品与发酵工业, 2021, 47(21): 46-52.
MA Wenjin,LI Meilin,WANG Bo,et al. Identification of Rhodotorula mucilaginosa CM-1 strain and isolation and purification of extracellular polysaccharide[J]. Food and Fermentation Industries, 2021, 47(21): 46-52.
链接本文:  
http://sf1970.cnif.cn/CN/10.13995/j.cnki.11-1802/ts.026645  或          http://sf1970.cnif.cn/CN/Y2021/V47/I21/46
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