Abstract: A method for the rapid determination of albendazole and its metabolites (albendazole sulfone, albendazole sulfoxide, albendazole-2-amino sulfone) residues in meat by high-performance liquid chromatography-tandem mass spectrometry (UHPLC-MS/MS) was developed. The samples were pretreated by acetonitrile and purified by QuEChERS reagent. The separation of albendazole and its metabolites residues was performed on an Agilent ZORBAX Eclipse Plus C18 column, and determined in the positive MRM modes by MS/MS using the external standard method. The results showed that the albendazole and its metabolites had good linear relationships within their respective linear ranges (R2>0.998) with the limits of quantification (LOQs) 0.1-1 μg/kg. At the three spiked concentration levels of 0.1-10 μg/kg, the average recovery of the intra-batch (n=6) was 81.9%-109.8%, the average recovery of the inter-batch (n=3) was 81.8%-99.0%, and the relative standard deviation (RSD) was 1.8%-14.4%. The method developed here was simple, fast, accurate, sensitive, reproducible and stable. And the limit of quantitation (LODs) and LOQs lower than the conventional detection methods, and this method could be used for the qualitative and quantitative analysis of albendazole and its metabolite residues in meat.
黄永桥,杨昌彪,马凯,等. QuEChERS结合UHPLC-MS/MS法测定畜肉中阿苯达唑及其代谢物的残留量[J]. 食品与发酵工业, 2021, 47(19): 258-263.
HUANG Yongqiao,YANG Changbiao,MA Kai,et al. Determination of albendazole and its metabolites residues in meat by UHPLC-MS/MS with QuEChERS extraction method[J]. Food and Fermentation Industries, 2021, 47(19): 258-263.
[1] DAVIS A,DIXON H,PAWLOWSKI Z S.Multicentre clinical trials of benzimidazole-carbamates in human cystic echinococcosis (phase 2)[J].Bulletin of the World Health Organization,1989,67(5):503-508. [2] 路宽,覃亮.抗寄生虫药阿苯达唑新活性研究[J].中国当代医药,2010,17(23):12-13. LU K,QIN L.Study on new activity of antiparasitic drug albendazole[J].China Modern Medicine,2010,17(23):12-13. [3] JONGSUKSUNTIGUL P,JERADIT C,PORNPATTANAKUL S,et al.A comparative study on the efficacy of albendazole and mebendazole in the treatment of ascariasis,hookworm infection and trichuriasis[J].The Southeast Asian Journal of Tropical Medicine and Public Health,1993,24(4):724-729. [4] 李晓婷, 周绪正,李冰,等.广谱抗蠕虫药阿苯达唑研究进展[J].中兽医医药杂志,2019,38(3):26-29. LI X T,ZHOU X Z,LI B,et al.Reviewed on research of albendazole[J].Journal of Traditional Chinese Veterinary Medicine,2019,38(3):26-29. [5] 中华人民共和国农业农村部,中华人民共和国国家卫生健康委员会,中华人民共和国国家市场监督管理总局.GB 31650—2019食品安全国家标准 食品中兽药最大残留限量[S].北京:中国标准出版社,2019. Ministry of Agriculture and Rural Areas of the People's Republic of China,National Health Commission of the People's Republic of China,State Administration of Quality Supervision.GB 31650—2019,National food safety standard,Maximum residue limits of veterinary drugs in food[S].Beijing:China Standards Press,2019. [6] 吴宁鹏, 彭丽,孟蕾,等.高效液相色谱-荧光法测定牛羊组织中阿苯达唑及其代谢物的残留量[J].中国兽药杂志,2017,51(2):35-39. WU N P,PENG L,MENG L,et al.Determination ofalbendazole and its main metabolites residues in cattle and ovine tissues by HPLC-FLD[J].Chinese Journal of Veterinary Drug,2017,51(2):35-39. [7] 杜红鸽,谭旭信,方忠意,等.高效液相色谱法测定动物肌肉中的阿苯达唑及其代谢物[J].中国兽药杂志,2010,44(1):52-55. DU H G,TAN X X,FANG Z Y,et al.Determination ofalbendazole and its metabolite in animal muscle by HPLC[J].Chinese Journal of Veterinary Drug,2010,44(1):52-55. [8] SHAIKH B,RUMMEL N,REIMSCHUESSEL R.Determination of albendazole and its major metabolites in the muscle tissues of Atlantic salmon,tilapia,and rainbow trout by high performance liquid chromatography with fluorometric detection[J].Journal of Agricultural and Food Chemistry,2003,51(11):3 254-3 259. [9] FLETOURIS D J,PAPAPANAGIOTOU E P,NAKOS D S,et al.Highlysensitive ion pair liquid chromatographic determination of albendazole marker residue in animal tissues[J].Journal of Agricultural and Food Chemistry,2005,53(4):893-898. [10] SARANER N,ÖZKAN G Y,GÜNEY B,et al.Determination of albendazole sulfoxide in human plasma by using liquid chromatography-tandem mass spectrometry[J].Journal of Chromatography B,2016,1 022:1-5. [11] 张小军, 郑斌,张虹,等.超高效液相色谱-串联质谱法测定草鱼肉中阿苯达唑及其代谢物残留[J].分析化学,2011,39(6):815-820. ZHANG X J,ZHENG B,ZHANG H,et al.Determination ofalbendzole and its metabolites in grass carp meat by ultra performance liquid chromatography-tandem mass spectrometry[J].Chinese Journal of Analytical Chemistry,2011,39(6):815-820. [12] 赵超群, 刘柱,徐潇颖,等.超高效液相色谱-串联质谱法测定奶粉中16种苯并咪唑类药物残留量[J].理化检验-化学分册,2017,53(7):765-770. ZHAO C Q,LIU Z,XU X Y,et al.UHPLC-MS/MS determination of residual amounts of 16 benzimidazoles in milk powder[J].Physical Testing and Chemical Analysis (Part B:Chemical Analysis),2017,53(7):765-770. [13] CHHONKER Y S,EDI C,MURRY D J.LC-MS/MS method for simultaneous determination of diethylcarbamazine,albendazole and albendazole metabolites in human plasma:Application to a clinical pharmacokinetic study[J].Journal of Pharmaceutical and Biomedical Analysis,2018,151:84-90. [14] 张海波, 黄琼,卢帅,等.UPLC-Q-TOF-MS法测定绵羊血浆中阿苯达唑及其3种代谢产物的浓度及药代动力学研究[J].新疆医科大学学报,2018,41(12):1 543-1 548. ZHANG H B,HUANG Q,LU S,et al.Simultaneous determination of albendazole and its three metabolites in sheep plasma by UPLC-Q-TOF-MS and pharmacokinetic study[J].Journal of Xinjiang Medical University,2018,41(12):1 543-1 548. [15] MARKUS J,SHERMA J.Method II.gas chromatographic/mass spectrometric confirmatory method for albendazole residues in cattle liver[J].Journal of AOAC International,1992,75(6):1 135-1 137. [16] 倪建秀, 陈涛,陈桂芳.QuEChERS方法在食用农产品农兽药残留检测中的应用进展[J].现代农药,2016,15(5):5-8;11. NI J X,CHEN T,CHEN G F,Research progress of QuEChERS for determination of pesticides and veterinary drugs residues in edible agro-products[J].Modern Agrochemicals,2016,15(5):5-8;11. [17] MOLINA-RUIZ J M,CIESLIK E,WALKOWSKA I.Optimization of the QuEChERS method for determination of pesticide residues in chicken liver samples by gas chromatography-mass spectrometry[J].Food Analytical Methods,2015,8(4):898-906. [18] 张爱芝, 王全林,曹丽丽,等.QuEChERS-超高效液相色谱-串联质谱法测定蔬菜中250种农药残留[J].色谱,2016,34(2):158-164. ZHANG A Z,WANG Q L,CAO L L,et al.Determination of 250 pesticide residues in vegetables using QuEChERS-ultra performance liquid chromatography-tandem mass spectrometry[J].Chinese Journal of Chromatography,2016,34(2):158-164. [19] RIZZETTI T M,KEMMERICH M,MARTINS M L,et al.Optimization of a QuEChERS based method by means of central composite design for pesticide multiresidue determination in orange juice by UHPLC-MS/MS[J].Food Chemistry,2016,196:25-33. [20] FAN S F,ZHAO P Y,YU C S,et al.Simultaneous determination of 36 pesticide residues in spinach and cauliflower by LC-MS/MS using multi-walled carbon nanotubes-based dispersive solid-phase clean-up[J].Food Additives & Contaminants:Part A,2014,31(1):73-82.