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食品与发酵工业  2021, Vol. 47 Issue (19): 258-263    DOI: 10.13995/j.cnki.11-1802/ts.026666
  分析与检测 本期目录 | 过刊浏览 | 高级检索 |
QuEChERS结合UHPLC-MS/MS法测定畜肉中阿苯达唑及其代谢物的残留量
黄永桥1, 杨昌彪2, 马凯2*, 毛敏霞1, 简银池1
1(贵州省检测技术研究应用中心,贵州 贵阳,550016)
2(贵州省分析测试研究院,贵州 贵阳,550016)
Determination of albendazole and its metabolites residues in meat by UHPLC-MS/MS with QuEChERS extraction method
HUANG Yongqiao1, YANG Changbiao2, MA Kai2*, MAO Minxia1, JIAN Yinchi1
1(Guizhou Testing Technology Research and Application Center,Guiyang 550016,China)
2(Guizhou Academy of Testing and Analysis,Guiyang 550016,China)
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摘要 该文建立了快速测定畜肉中阿苯达唑及其代谢物(阿苯达唑砜、阿苯达唑亚砜、阿苯达唑-2-氨基砜)残留量的超高效液相色谱-串联质谱的分析方法。试样用乙腈提取,经QuEChERS试剂净化,通过Agilent ZORBAX Eclipse Plus C18色谱柱分离,在正离子多反应监测模式下分析,外标法定量。实验结果表明,阿苯达唑及其代谢物在各自的线性范围内线性关系良好(R2>0.998),检出限0.1~1 μg/kg,在空白样品中进行3个浓度水平的添加实验,添加范围为0.1~10 μg/kg,日内(n=6)平均回收率在81.9%~109.8%,日间(n=3)平均回收率在81.8%~99.0%,精密度为1.8%~14.4%。该方法操作简单、快速、准确、灵敏度高,重现性好,回收率高且稳定,检测限及定量限低于现有检测方法要求,可用于畜肉中阿苯达唑及其代谢物残留量的定性和定量分析。
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黄永桥
杨昌彪
马凯
毛敏霞
简银池
关键词:  QuEChERS  超高效液相色谱-串联质谱法  畜肉  阿苯达唑及其代谢物    
Abstract: A method for the rapid determination of albendazole and its metabolites (albendazole sulfone, albendazole sulfoxide, albendazole-2-amino sulfone) residues in meat by high-performance liquid chromatography-tandem mass spectrometry (UHPLC-MS/MS) was developed. The samples were pretreated by acetonitrile and purified by QuEChERS reagent. The separation of albendazole and its metabolites residues was performed on an Agilent ZORBAX Eclipse Plus C18 column, and determined in the positive MRM modes by MS/MS using the external standard method. The results showed that the albendazole and its metabolites had good linear relationships within their respective linear ranges (R2>0.998) with the limits of quantification (LOQs) 0.1-1 μg/kg. At the three spiked concentration levels of 0.1-10 μg/kg, the average recovery of the intra-batch (n=6) was 81.9%-109.8%, the average recovery of the inter-batch (n=3) was 81.8%-99.0%, and the relative standard deviation (RSD) was 1.8%-14.4%. The method developed here was simple, fast, accurate, sensitive, reproducible and stable. And the limit of quantitation (LODs) and LOQs lower than the conventional detection methods, and this method could be used for the qualitative and quantitative analysis of albendazole and its metabolite residues in meat.
Key words:  QuEChERS    ultra high performance liquid chromatography-tandem mass-spectrometry    meat    albendazole and its metabolites
收稿日期:  2021-01-18      修回日期:  2021-03-16                发布日期:  2021-11-04      期的出版日期:  2021-10-15
基金资助: 国家重点研发计划(2017YFC1601800);贵州省高层次创新型“千”层次人才培养
作者简介:  学士,工程师(马凯工程师为通讯作者,E-mail:makai@gzata.cn)
引用本文:    
黄永桥,杨昌彪,马凯,等. QuEChERS结合UHPLC-MS/MS法测定畜肉中阿苯达唑及其代谢物的残留量[J]. 食品与发酵工业, 2021, 47(19): 258-263.
HUANG Yongqiao,YANG Changbiao,MA Kai,et al. Determination of albendazole and its metabolites residues in meat by UHPLC-MS/MS with QuEChERS extraction method[J]. Food and Fermentation Industries, 2021, 47(19): 258-263.
链接本文:  
http://sf1970.cnif.cn/CN/10.13995/j.cnki.11-1802/ts.026666  或          http://sf1970.cnif.cn/CN/Y2021/V47/I19/258
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