Abstract: A method for the rapid determination of albendazole and its metabolites (albendazole sulfone, albendazole sulfoxide, albendazole-2-amino sulfone) residues in meat by high-performance liquid chromatography-tandem mass spectrometry (UHPLC-MS/MS) was developed. The samples were pretreated by acetonitrile and purified by QuEChERS reagent. The separation of albendazole and its metabolites residues was performed on an Agilent ZORBAX Eclipse Plus C18 column, and determined in the positive MRM modes by MS/MS using the external standard method. The results showed that the albendazole and its metabolites had good linear relationships within their respective linear ranges (R2>0.998) with the limits of quantification (LOQs) 0.1-1 μg/kg. At the three spiked concentration levels of 0.1-10 μg/kg, the average recovery of the intra-batch (n=6) was 81.9%-109.8%, the average recovery of the inter-batch (n=3) was 81.8%-99.0%, and the relative standard deviation (RSD) was 1.8%-14.4%. The method developed here was simple, fast, accurate, sensitive, reproducible and stable. And the limit of quantitation (LODs) and LOQs lower than the conventional detection methods, and this method could be used for the qualitative and quantitative analysis of albendazole and its metabolite residues in meat.
黄永桥,杨昌彪,马凯,等. QuEChERS结合UHPLC-MS/MS法测定畜肉中阿苯达唑及其代谢物的残留量[J]. 食品与发酵工业, 2021, 47(19): 258-263.
HUANG Yongqiao,YANG Changbiao,MA Kai,et al. Determination of albendazole and its metabolites residues in meat by UHPLC-MS/MS with QuEChERS extraction method[J]. Food and Fermentation Industries, 2021, 47(19): 258-263.
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