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食品与发酵工业  2021, Vol. 47 Issue (20): 105-113    DOI: 10.13995/j.cnki.11-1802/ts.026670
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淡竹叶盐对脂多糖诱导的RAW264.7细胞炎症抑制作用和肝细胞生长影响的研究
季红福1, 赵雯雯1, 王丹洪1, 郑文豪1, 肖竹钱1, 戴静1, 王永江1*, 毛建卫1,2*
1(浙江科技学院 生物与化学工程学院,浙江省农产品化学与生物加工技术重点实验室,浙江省农业生物资源生化制造协同中心,浙江 杭州,310023)
2(浙江工业职业技术学院,浙江 绍兴,312000)
Effects of lophatherum gracile salt on lipopolysaccharide-induced inflammation in RAW264.7 cells and hepatocyte proliferation
JI Hongfu1, ZHAO Wenwen1, WANG Danhong1, ZHENG Wenhao1, XIAO Zhuqian1, DAI Jing1, WANG Yongjiang1*, MAO Jianwei1,2*
1(Zhejiang University of Science & Technology, Zhejiang Provincial Key Lab for Chem&Bio Processing Technology of Farm Product, Zhejiang Provincial Collaborative Innovation Center of Agricultural Biological Resources Biochemical Manufacturing, School of Biological and Chemical Engineering, Hangzhou 310023, China)
2(Zhejiang Industry Polytechnic College, Shaoxing 312000, China)
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摘要 为了探究淡竹叶盐成分对脂多糖诱导的RAW264.7细胞炎症抑制作用和肝细胞生长影响,采用竹盐制备工艺制备淡竹叶盐,对比分析粗海盐、精制海盐、传统竹盐和淡竹叶盐的pH、矿物元素含量等理化性质,并探究炎症抑制效果及对不同肝细胞生长的影响。结果表明,淡竹叶盐水溶液呈碱性,矿物元素组成丰富,能显著增强RAW264.7细胞吞噬能力,抑制NO释放以及降低肿瘤坏死因子-α、白细胞介素-1β分泌;显著抑制肝癌细胞(HepG2)的增殖,且淡竹叶盐对HepG2细胞总凋亡率[(45.85±2.93)%]高于传统竹盐[(38.89±2.56)%]、粗海盐[(31.78±4.18)%]和精制海盐[(29.63±1.89)%];而在同质量浓度下,淡竹叶盐对正常肝细胞(WRL68)的凋亡率[(16.98±2.84)%]与竹盐[(15.21±3.10)%]无明显差异,显著低于粗海盐[(22.35±2.24)%]和精制海盐[(19.35±3.12)%],其对正常肝细胞保护作用强于粗海盐和精制海盐。
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季红福
赵雯雯
王丹洪
郑文豪
肖竹钱
戴静
王永江
毛建卫
关键词:  淡竹叶盐  矿物元素  抗炎活性  增殖  凋亡    
Abstract: To explore the inhibitory effect of lophatherum gracile salt on LPS induced RAW264.7 cell inflammation and hepatocyte growth, lophatherum gracile salt was firstly prepared using the bamboo salt preparation process. Then the physicochemical properties of crude sea salts, refined sea salt, traditional bamboo salt and lophatherum gracile salt, such as pH and mineral element content, were analyzed. And the inhibitory effect of inflammation and its influence on different hepatocyte growth were explored. The results showed that the salt solution of lophatherum gracile salt was alkaline, which riches in mineral elements. It could significantly enhance the phagocytic capacity of RAW264.7 cells and inhibit the release of NO. Moreover, it also reduced the secretion of TNF-α and IL-1β and inhibited the proliferation of HepG2 hepatoma cells. The total apoptosis rate of HepG2 cells induced by lophatherum gracile salt [(45.85±2.93)%] was higher than that of traditional bamboo salt [(38.89±2.56)%], crude sea salt [(31.78±4.18)%] and refined sea salt [(29.63±1.89)%]. At the same concentration, there was no significant difference between the apoptosis rate of normal hepatocytes [(16.98±2.84)%] and that of bamboo salt [(15.21±3.10)%], which was significantly lower than that of crude sea salt [(22.35±2.24)%] and refined sea salt [(19.35±3.12)%]. Its protective effect on normal hepatocytes was stronger than that of crude sea salt and refined sea salt.
Key words:  lophatherum gracile salt    mineral elements    anti-inflammatory activity    proliferation    apoptosis
收稿日期:  2021-01-12      修回日期:  2021-03-11           出版日期:  2021-10-25      发布日期:  2021-11-18      期的出版日期:  2021-10-25
基金资助: 浙江省公益技术应用研究计划项目(LGC21B060001);浙江省自然科学基金(LZY21E080002)
作者简介:  硕士研究生(王永江教授和毛建卫教授级高级工程师为共同通讯作者,E-mail:yjwang@zust.edu.cn;zjhzmjw@163.com)
引用本文:    
季红福,赵雯雯,王丹洪,等. 淡竹叶盐对脂多糖诱导的RAW264.7细胞炎症抑制作用和肝细胞生长影响的研究[J]. 食品与发酵工业, 2021, 47(20): 105-113.
JI Hongfu,ZHAO Wenwen,WANG Danhong,et al. Effects of lophatherum gracile salt on lipopolysaccharide-induced inflammation in RAW264.7 cells and hepatocyte proliferation[J]. Food and Fermentation Industries, 2021, 47(20): 105-113.
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http://sf1970.cnif.cn/CN/10.13995/j.cnki.11-1802/ts.026670  或          http://sf1970.cnif.cn/CN/Y2021/V47/I20/105
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