Abstract: Streptococcus thermophilus isa major starter in industry and widely used in fermented products. The technology of high-speed performance liquid chromatography-quadrupole-time of flight mass spectrometry was used to monitor the dynamic changes. The results showed that combined with database search and mass spectrometry information matching, a total of 24 significantly different metabolites were screened out including pyridoxamine, L-lysine, tryptophan, etc.S.thermophilus S4 produced many small molecular compounds, which together endowed fermented milk with unique flavor and rich nutritional value.S.thermophilus S4 is able to be used as a potential starter in the production of dairy products.
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