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食品与发酵工业  2021, Vol. 47 Issue (16): 123-127    DOI: 10.13995/j.cnki.11-1802/ts.026873
  研究报告 本期目录 | 过刊浏览 | 高级检索 |
南极磷虾油对衰老模型小鼠抗氧化作用的研究
李辉1*, 王廉馨1, 韩昀稣1, 林丹妮1, 曲丽娜2
1(辽宁大学 生命科学院,辽宁 沈阳,110036)
2(大连医科大学附属大连市中心医院,辽宁 大连,116033)
Antioxidant effect of Antarctic krill oil on aging mice
LI Hui1*, WANG Lianxin1, HAN Yunsu1, LIN Danni1, QU Lina2
1(School of Life Sciences, Liaoning University, Shenyang 110036, China)
2(Dalian Municipal Central Hospital Affiliated of Dalian Medical University, Dalian 116033, China)
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摘要 该文研究了南极磷虾油(antarctic krill oil,AKO)对衰老模型小鼠的抗氧化作用。无特定病原体(specific pathogen free,SPF)级癌症研究学会(institute of cancer research,ICR)健康雄性小鼠培养达到8~10月龄构建衰老模型小鼠,将衰老模型小鼠随机分成4组,每组10只,设置南极磷虾油处理低剂量组(L-AKO)0.075 g/kg·BW、中剂量组(M-AKO)0.150 g/kg·BW、高剂量组(H-AKO)0.450 g/kg·BW和阴性对照组,阴性对照组给予等体积的食用植物油,每天灌胃1次,连续灌胃30 d。30 d后,测定过氧化脂质(lipid peroxide,LPO)含量[以丙二醛(malondialdehyde,MDA)含量计]、还原型谷胱甘肽(reduced glutathione,GSH)含量、超氧化物歧化酶(superoxide dismutase,SOD)活力及蛋白质羰基(protein carbonyl,PCO)含量。结果表明,AKO中、高剂量组能分别显著和极显著提高血清SOD活力(P<0.05,P<0.01)和GSH含量(P<0.05,P<0.01),显著降低血液中LPO含量(P<0.05),PCO含量无显著变化(P>0.05)。研究证明AKO对衰老模型小鼠具有抗氧化作用。
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李辉
王廉馨
韩昀稣
林丹妮
曲丽娜
关键词:  南极磷虾油  衰老模型小鼠  抗氧化作用  虾青素  磷脂    
Abstract: The aim of this study was to explore the antioxidant effect of Antarctic krill oil (AKO) on aging model mice. SPF-ICR healthy male mice aged 8-10 months were randomly divided into 4 groups, low-dose group, medium-dose group, high-dose krill oil group and one negative control group. Ten mice in each group and were given a dose of 0.075 g/kg·BW, 0.150 g/kg·BW, 0.450 g/kg·BW and corn germ oil respectively. The four mice groups were dosed by gavage once a day for 30 days. After 30 days, the contents of lipid peroxide (LPO) (calculated by the content of MDA), reduced glutathione (GSH), superoxide dismutase (SOD) and protein carbonyl (PCO) were determined. The results showed that compared with the control group, the activity of serum SOD and serum level of GSH increased considerably (P<0.05) in the medium dose of the krill oil group, and even significantly (P<0.01) in the high-dose group. The level of LPO in the medium dose group was lower (P<0.05) than the control group. However, there was no statistical difference for the level of PCO between the medium dose group and the control group (P>0.05). These results revealed AKO has an antioxidant effect on aging model mice.
Key words:  Antarctic krill oil    aging model mice    antioxidation    astaxanthin    phospholipids
收稿日期:  2021-02-02      修回日期:  2021-04-15                发布日期:  2021-09-10      期的出版日期:  2021-08-25
作者简介:  高级实验师(本文通讯作者,E-mail:huili@lnu.edu.cn)
引用本文:    
李辉,王廉馨,韩昀稣,等. 南极磷虾油对衰老模型小鼠抗氧化作用的研究[J]. 食品与发酵工业, 2021, 47(16): 123-127.
LI Hui,WANG Lianxin,HAN Yunsu,et al. Antioxidant effect of Antarctic krill oil on aging mice[J]. Food and Fermentation Industries, 2021, 47(16): 123-127.
链接本文:  
http://sf1970.cnif.cn/CN/10.13995/j.cnki.11-1802/ts.026873  或          http://sf1970.cnif.cn/CN/Y2021/V47/I16/123
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