Abstract: Betaine can effectively improve the fermentation performance of strains in industrial production. The effect of betaine on threonine fermentation and intracellular metabolism of Escherichia coli THRD were investigated. The threonine titer increased from 50.74 g/L to 54.59 g/L, while the yield increased from 37.45% to 40.69% when betaine was fed. In such case, 28 significantly different metabolites related threonine anabolism were discovered. Metabolic flux analysis showed that carbon flow distribution of glycolysis and pentose phosphate pathway were changed, CO2 fixation reaction catalyzed by phosphoenol pyruvate carboxylase and tricarboxylic acid cycle were regulated, and bypass metabolism of glyceraldehyde-3-phosphate, aspartic acid and threonine were reduced, thus providing sufficient reducing power and precursor for threonine synthesis. The results revealed the effect of betaine on E. coli on the metabolic level and provided a theoretical reference for the application of betaine in other industrial fermentation products.
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LI Jie,TIAN Junyu,JI Yuanqing,et al. Effect of betaine on synthesis of threonine in Escherichia coli by metabonomic analysis[J]. Food and Fermentation Industries, 2021, 47(17): 34-40.
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2021, Vol. 47 
