Abstract: A method for the determination of dimethipin residues in animal derived food by high performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS) was developed. The samples were extracted with acetonitrile containing 1% (V/V) acetic acid and defatted with n-hexane, followed by clean-up using C18 solid phase extraction column. The analytes were separated on a Venusil MP C18(2) column (150 mm×2.1 mm, 3 μm) under a gradient elution program using acetonitrile and water was used as the mobile phases. The analytes were detected by tandem mass spectrometry using a negative atmospheric pressure chemical ionization (APCI-) source in the multiple reaction monitoring (MRM) mode. The internal standard calibration curves were used for quantitative analysis. The results showed that calibration curves had good linearities in the range of 1.0-500.0 μg/L for dimethipin, which with a correlation coefficients (R2) 0.999 3. The limit of quantification (LOQ) of the method was 1.0 μg/kg. The average recoveries of dimethipin in pork, pig liver, beef, beef liver, mutton, sheep liver, chicken, chicken gizzard, egg and milk matrices at spiked levels of 1.0, 10.0 and 100.0 μg/kg were 89.0%-105.0%, and the relative standard deviations were 2.20%-7.64%. The method has less matrix interference, with high sensitivity, accuracy and reliability, and it is suitable for the quantitative detection of dimethipin residues in animal derived food.
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