Study on the kinetics of amino nitrogen production and quality changes by Aspergillus-type Douchi at different post-fermentation temperatures
WANG Di1,2,3, WANG Zhirong1,2,3, CHEN Xuhui1,2,3, SONG Jun4, KONG Xiangbing4, CHEN Benkai4, KAN Jianquan1,2,3*
1(College of Food Science,Southwest University,Chongqing 400715,China) 2(Laboratory of Quality & Safety Risk Assessment for Agro-products on Storage and Preservation (Chongqing), Ministry of Agriculture and Rural Affairs,PRC,Chongqing 400715,China) 3(Chinese-Hungarian Cooperative Research Centre for Food Science,Chongqing 400715,China) 4(Junyi Food Factory,Yongchuan District,Chongqing 402160,China)
Abstract: Post-fermentation is a key stage for the special color,flavor and nutrients formation in Aspergillus-type Douchi,the extent of which is usually determined by an important indicator-amino nitrogen (AN).To investigate the influences of post-fermentation temperature on the AN formation kinetics as well as the quality of Aspergillus-type Douchi,the AN content change of four soybeans (three yellow soybeans and one black soybean) were measured at different post-fermentation temperatures,and fitted by the modified-Gompertz equation in this study.Moisture content,pH value,browning index (BI),total acid (TA),AN and free amino acids (FAA) contents were measured,and the correlation of these indicators was analyzed.The results showed that the modified-Gompertz equation could well describe the kinetics of AN production (R2>0.94) for the four different Aspergillus-type Douchi at the post-fermentation temperature ranging from 45 ℃ to 65 ℃.Though the maximum content values and production rates of AN were getting lower as the temperature increased,there was no significant difference in these indexes among the four Douchi.According to the correlation analysis,four Douchi have implied a consistent trend:temperature was positively correlated with BI (P<0.01),while negatively correlated with TA and AN (P<0.01).Moreover,the FAA content of the four kinds of Douchi decreased with the increase of temperature.Our work may provide theoretical support for the post-fermentation process of Aspergillus-type Douchi.
王迪,王智荣,陈湑慧,等. 不同后发酵温度下曲霉型豆豉的氨基酸态氮生成动力学及品质变化研究[J]. 食品与发酵工业, 2021, 47(9): 91-99.
WANG Di,WANG Zhirong,CHEN Xuhui,et al. Study on the kinetics of amino nitrogen production and quality changes by Aspergillus-type Douchi at different post-fermentation temperatures[J]. Food and Fermentation Industries, 2021, 47(9): 91-99.
[1] 阚建全, 陈宗道,石轶松,等.豆豉非透析类黑精抗氧化和抑制亚硝胺合成的研究[J].营养学报,1999,21(3):349-352. KAN J Q,CHEN Z D,SHI Y S,et al.Study on antioxidation and inhibition of nitrosamine synthesis by Douchi nondialyzable melanoidin[J].Acta Nutrimenta Sinica,1999,21(3):349-352. [2] 索化夷, 赵欣,骞宇,等.永川豆豉发酵过程中总糖和氨基酸变化与滋味的形成[J].食品科学,2015,36(21):100-104. SUO H Y,ZHAO X,QIAN Y,et al.Changes in total sugar and amino acids,and formation of taste compounds in Yongchuan Douchi during fermentation[J].Food Science,2015,36(21):100-104. [3] ZHANG W,LIU X,YANG Z,et al.Effect of soaking and temperature process on the volatile compounds in soymilk made by soymilk maker[J].Journal of Food Science and Technology,2018,55(4):1 591-1 598. [4] 李金林, 万亮,王维亚,等.基于SPME-GC-MS技术分析曲霉型豆豉生产过程中的挥发性成分变化[J].食品与发酵工业,2020,46(15):252-257. LI J L,WAN L,WANG W Y,et al.Change of volatile compounds in Aspergillus-type Douchi during processing evaluated by SPME-GC-MS[J].Food and Fermentation Industries,2020,46(15):252-257. [5] 汤启成, 曾凡玉,汪学荣,等.毛霉型豆豉与曲霉型豆豉挥发性成分的GC-MS分析[J].食品工业,2016,37(10):274-278. TANG Q C,ZENG F Y,WANG X R,et al.GC-MS analysis of volatile components in Mucor-fermented soybean and Aspergillus-fermented soybean[J].The Food Industry,2016,37(10):274-278. [6] 宋永生. 豆豉加工前后营养与活性成分变化的研究[J].食品工业科技,2003,24(7):79-80;64. SONG Y S.Study on the changes of nutrition and active components of tempeh before and after processing[J].Science and Technology of Food Industry,2003,24(7):79-80;64. [7] 索化夷, 卢露,吴佳敏,等.永川豆豉在传统发酵过程中基本成分及蛋白酶活性变化[J].食品科学,2011,32(1):177-180. SUO H Y,LU L,WU J M,et al.Changes in basal components and protease activity at different stages of the traditional processing of Yongchuan Douchi[J].Food Science,2011,32(1):177-180. [8] LIU H,LV Y XU J T,et al.Soybean peptide aggregates improved calcium binding capacity[J].LWT-Food Science and Technology,2016,67:174-180. [9] 陈怡, 陆敏捷,刘洋,等.不同类型豆豉抗氧化活性差异与影响因素分析[J].食品与发酵工业,2020,46(13):36-41. CHEN Y,LU M J,LIU Y,et al.Analysis of antioxidant activity of ten kinds of Douchi and their influencing factors[J].Food and Fermentation Industries,2020,46(13):36-41. [10] SHIN Z I,YU R,PARK S A,et al.His-His-Leu,an angiotensin I converting enzyme inhibitory peptide derived from Korean soybean paste,exerts antihypertensive activity in vivo[J].Journal of Agricultural and Food Chemistry,2001,49(6):3 004-3 009. [11] 张仁凤. 霉菌纯种制曲豆豉在发酵过程中生物胺的变化研究[D].重庆:西南大学,2018. ZHANG R F.Study on the changes of biogenic amines in Douchi by pure starter-making with mould during the fermentation process[D].Chongqing:Southwest University,2018. [12] 吴枚枚. 毛霉豆豉生产工艺的研究及其新产品开发[D].成都:成都大学,2020. WU M M.Research on production process and new product development of Mucor Douchi[D].Chengdu:Chengdu University,2020. [13] MAHDINIA E,MAMOURI S J,PURI V M,et al.Modeling of vitamin K (menaquinoe-7) fermentation by Bacillussubtilis natto in biofilm reactors[J].Biocatalysis and Agricultural Biotechnology,2019,17:196-202. [14] CHOHAN N A,ARUWAJOYE G S,SEWSYNKER-SUKAI Y,et al.Valorisation of potato peel wastes for bioethanol production using simultaneous saccharification and fermentation:process optimization and kinetic assessment[J].Renewable Energy,2020,146:1 031-1 040. [15] COSTA R,FUSCO F M,GANDARA J F M.Mass transfer dynamics in soaking of chickpea[J].Journal of Food Engineering,2018,227:42-50. [16] 中华人民共和国国家质量监督检验检疫总局.GB/T 12456—2008 食品中总酸的测定[S].北京:中国标准出版社,2008. General Administration of Quality Supervision,Inspection and Quarantine of the People's Republic of China.GB/T 12456—2008 Determination of total acid in food[S].Beijing:China Standard Press,2008. [17] 中华人民共和国国家卫生和计划生育委员会.GB 5009.235—2016 食品安全国家标准食品中氨基酸态氮的测定[S].北京:中国标准出版社,2016. National Health and Family Planning Commission of the People's Republic of China.GB 5009.235—2016 National food safety standard determination of amino acid nitrogen in food[S].Beijing:China Standard Press,2016. [18] KIM M J,KWAK H S,KIM S S.Effects of salinity on bacterial communities,Maillard reactions,isoflavone composition,antioxidation and antiproliferation in Korean fermented soybean paste (doenjang)[J].Food Chemistry,2018,245:402-409. [19] 梁叶星, 张玲,高飞虎,等.重庆水豆豉发酵过程中NaCl,还原糖和氨基酸变化与滋味的形成[J].食品与发酵工业,2019,45(18):27-34. LIANG Y X,ZHANG L,GAO F H,et al.Changes in NaCl,reducing sugar and amino acids and formation of tastes in Chongqing Shuidouchi during fermentation[J].Food and Fermentation Industries,2019,45(18):27-34. [20] 赵英莲, 牟德华,李艳.2,4-二硝基氟苯柱前衍生HPLC检测树莓中游离氨基酸[J].食品科学,2015,36(6):178-182. ZHAO Y L,MOU D H,LI Y.High performance liquid chromatographic analysis of free amino acids in raspberry using pre-column derivatization with 2,4-dinitrofluorobenzene[J].Food Science,2015,36(6):178-182. [21] 孙森. 天然发酵豆豉后发酵过程的动态分析[D].济南:山东轻工业学院,2008. SUN S.Dynamic analysis on later fermentation process of Douchi natural fermentation[D].Jinan:Shandong Institute of Light Industry,2008. [22] CHOACHAMNAN J,MORARU C,LEE S,et al.Multiple-factor mathematical modeling of glycine-glucose browning[J].Journal of Food Engineering,2020,273:109 829. [23] 赵文鹏. 曲霉型豆豉发酵过程中品质特性及微生物变化规律研究[D].南昌:江西师范大学,2020. ZHAO W P.Quality characteristics and microbial community evolution of Aspergillus-type Douchi during fermentation[D].Nanchang:Jiangxi Normal University,2020. [24] 秦礼康. 陈窖豆豉粑益酵菌,风味物及黑色物质研究[D].无锡:江南大学,2006. QIN L K.Studies on fermentative microflora,flavours and melanoidins of long-ripined Douchiba[D].Wuxi:Jiangnan University,2006. [25] 韩佳冬. 永川豆豉蛋白水解产物与黑色素形成机理的研究[D].重庆:西南大学,2013. HAN J D.The research on protein hydrolysate of yongchuan Douchi and melanin formation[D].Chongqing:Southwest University,2013.