Abstract: In order to obtain excellent indigenous Hanseniaspora strains with low ethanol yield, fifty-three Hanseniaspora strains selected from the wine-producing region of Ningxia, Gansu, Shaanxi and Xinjiang were evaluated. Hanseniaspora opuntiae HoA-1-3, HoC-4-2 and Hanseniaspora uvarum HuB-2-2, HuC-3-2 were screened. The results showed that the ethanol yield of strains HoA-1-3, HoC-4-2, HuB-2-2 and HuC-3-2 were reduced by 23.87%, 19.25%, 10.74% and 18.74%, respectively, compared with Saccharomyces cerevisiae NX11424. The four strains displayed good tolerance to 400 g/L of sugar, 300 mg/L of SO2, pH 2.8, extreme temperatures 10℃ and 40℃, and no killing activity against S. cerevisiae NX11424. In addition, HoA-1-3 and HoC-4-2 could tolerate 6% vol of ethanol. HoC-4-2 and HuC-3-2 did not produce H2S. In conclusion, H. opuntiae HoC-4-2 and H. uvarum HuC-3-2 had good oenological properties, which laid a foundation for further development and application of non-Saccharomyces yeast with low ethanol yield.
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