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食品与发酵工业  2021, Vol. 47 Issue (21): 53-58    DOI: 10.13995/j.cnki.11-1802/ts.027084
  研究报告 本期目录 | 过刊浏览 | 高级检索 |
蜡样芽胞杆菌BC307的分离鉴定及其RsiP蛋白的抗体制备
郑庆芳1,2, 刘丽娜3, 刘先凯2, 吕宇飞2, 王恒樑1,2, 王东澍2*, 邱泽武4*
1(上海海洋大学 食品学院,上海,201306)
2(军事科学院军事医学研究院生物工程研究所 病原微生物生物安全国家重点实验室,北京,100071)
3(兴安盟人民医院,内蒙古 乌兰浩特,137400)
4(解放军总医院第五医学中心(南院区)中毒救治科,北京,100071)
Identification of Bacillus cereus BC307 and the antibody preparation against RsiP
ZHENG Qingfang1,2, LIU Lina3, LIU Xiankai2, LYU Yufei2, WANG Hengliang1,2, WANG Dongshu2*, QIU Zewu4*
1(College of Food, Shanghai Ocean University, Shanghai 201306, China)
2(State Key Laboratory of Pathogen and Biosecurity, Institute of Biotedchnology, Beijing 100071, China)
3(Xing’an League People’s Hospital, Ulanhot 137400, China)
4(Department of Poisoning Treatment, the Fifth Medical Center of PLA General Hospital, Beijing 100071, China)
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摘要 蜡样芽胞杆菌(Bacillus cereus)是一种常见的食源性致病菌。从食物中毒病人的食物残渣中分离到1株产呕吐毒素的蜡样芽胞杆菌BC307,其对青霉素类抗生素具有较强的抗性。抗SigP因子RsiP是蜡样芽胞杆菌族(Bacillus cereus sensu lato)细菌青霉素耐药的重要调控因子。该实验利用原核系统表达RsiP蛋白并利用亲和层析法进行纯化,免疫小鼠后获得高效价多克隆抗体。结果表明,RsiP蛋白在炭疽芽胞杆菌、蜡样芽胞杆菌、苏云金芽胞杆菌中高度同源,在Western Blotting实验中RsiP抗血清与这3种菌的全菌蛋白均能发生特异性免疫反应。研究结果为探究RsiP对蜡样芽胞杆菌群细菌的青霉素耐药性调控打下了良好的基础。
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郑庆芳
刘丽娜
刘先凯
吕宇飞
王恒樑
王东澍
邱泽武
关键词:  蜡样芽胞杆菌  分离鉴定  青霉素耐药  RsiP  抗体制备    
Abstract: Bacillus cereus is a common food-borne pathogen and anti-SigP factor RsiP is an important regulator of penicillin resistance. B. cereus BC307 strain from the food residues of food poisoning patient was isolated, which has strong resistance to penicillin. The RsiP protein was expressed and purified by affinity chromatography. High-titer polyclonal antibody was obtained after immunizing mice. The results showed that the RsiP protein was highly conserved and identical in B. anthracis, B. cereus, and B. thuringiensis. Western blotting showed that the RsiP antiserum had specific immunological reactions with the whole bacterial protein of these three bacteria. The studies lay a good foundation for exploring the regulation of penicillin resistance of B. cereus by RsiP.
Key words:  Bacillus cereus    isolation and identification    penicillin resistance    RsiP    antibody preparation
收稿日期:  2021-02-23      修回日期:  2021-04-07           出版日期:  2021-11-15      发布日期:  2021-11-30      期的出版日期:  2021-11-15
基金资助: 国家自然科学基金项目(81871619)
作者简介:  硕士研究生(王东澍助理研究员和邱泽武主任医师为共同通讯作者,E-mail:wangdongshu@foxmail.com;qiuzw828@163.com)
引用本文:    
郑庆芳,刘丽娜,刘先凯,等. 蜡样芽胞杆菌BC307的分离鉴定及其RsiP蛋白的抗体制备[J]. 食品与发酵工业, 2021, 47(21): 53-58.
ZHENG Qingfang,LIU Lina,LIU Xiankai,et al. Identification of Bacillus cereus BC307 and the antibody preparation against RsiP[J]. Food and Fermentation Industries, 2021, 47(21): 53-58.
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