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食品与发酵工业  2021, Vol. 47 Issue (24): 77-82    DOI: 10.13995/j.cnki.11-1802/ts.027163
  研究报告 本期目录 | 过刊浏览 | 高级检索 |
耐硒菌株Lysinibacillus fusiformis D1的鉴定及清除自由基能力研究
段玉桦1,3, 余雍和1,3, 杨锐1,3, 王一丹1,3, 周欣1,3, 王璋倩2,3*
1(武汉轻工大学 生命科学与技术学院,湖北 武汉,430023)
2(武汉轻工大学 硒科学与工程现代产业学院,湖北 武汉,430040)
3(武汉轻工大学,国家富硒农产品加工技术研发专业中心,湖北 武汉,430040)
Screening and identification of selenium-tolerant Lysinibacillus fusiformis D1 and scavenging activity on oxygen free radicals
DUAN Yuhua1,3, YU Yonghe1,3, YANG Rui1,3, WANG Yidan1,3, ZHOU Xin1,3, WANG Zhangqian2,3*
1(College of Life Science and Technology, Wuhan Polytechnic University, Wuhan 430023, China)
2(School of Modern Industry for Selenium Science and Engineering, Wuhan Polytechnic University, Wuhan 430040, China)
3(National R & D Center for Se-Rich Agricultural Products Processing, Wuhan Polytechnic University, Wuhan 430040, China)
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摘要 硒污染土壤对生态环境和人体健康存在危害,国内利用微生物修复高硒土壤污染研究较少。从江汉平原土壤中以耐硒能力筛选出1株高耐硒菌株,对该菌株进行鉴定、生长动力学分析和纳米硒表征,并对其清除自由基活性进行评价。结果表明,该菌株为纺锤形赖氨酸芽孢杆菌,对亚硒酸钠最高耐受性为35 mg/mL。纳米硒粒径为(306.20±1.64)nm。菌株次生代谢产物有一定DPPH自由基清除能力[(66.38±0.67)%]和较强ABTS阳离子自由基清除能力[(99.94±0.08)%],该菌株制备纳米硒有一定羟自由基清除能力[(59.81±5.09)%]。该菌株可为富硒土壤污染的生物修复提供参考,其次生代谢产物中清除自由基物质发掘有待深入研究。
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段玉桦
余雍和
杨锐
王一丹
周欣
王璋倩
关键词:  纺锤形赖氨酸芽孢杆菌  耐硒  纳米硒  生物修复  自由基    
Abstract: Selenium-contaminated soil may be harmful to the ecological environment and human health. However, there are few studies on the use of microorganisms as a remedy in China. A high selenium tolerance strain was isolated from the soil of the Jianghan Plain. The strain was identified, its growth kinetics were analyzed, its nano-selenium was characterized, and its free radical scavenging activity was evaluated. The results showed that strain D1 was Lysinibacillus fusiformis and the highest tolerance to sodium selenite was 35 mg/mL. The particle size of nano-selenium was (306.20 ±1.64) nm. The secondary metabolites of Lysinibacillus fusiformis D1 had certain DPPH radical scavenging ability (66.38±0.67)% and strong ABTS radical scavenging ability (99.94±0.08)%. The nano-selenium produced by this strain had certain hydroxyl radical scavenging ability, which was (59.81±5.09)%. This strain can provide a reference for the bioremediation of selenium-rich soils, and the scavenging free radicals in its secondary metabolites need to be further studied.
Key words:  Lysinibacillus fusiformis    Se-tolerance    SeNPs    biological recovery    free radical
收稿日期:  2021-03-04      修回日期:  2021-04-09           出版日期:  2021-12-25      发布日期:  2022-01-21      期的出版日期:  2021-12-25
基金资助: 国家自然科学基金项目(31900011);武汉轻工大学科研项目资助(2019y05);武汉轻工大学大学生科研项目(xsky2020028);湖北省“楚天学者计划”人才项目
作者简介:  硕士研究生(王璋倩副教授为通讯作者,E-mail:wzqsnu@whpu.edu.cn)
引用本文:    
段玉桦,余雍和,杨锐,等. 耐硒菌株Lysinibacillus fusiformis D1的鉴定及清除自由基能力研究[J]. 食品与发酵工业, 2021, 47(24): 77-82.
DUAN Yuhua,YU Yonghe,YANG Rui,et al. Screening and identification of selenium-tolerant Lysinibacillus fusiformis D1 and scavenging activity on oxygen free radicals[J]. Food and Fermentation Industries, 2021, 47(24): 77-82.
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http://sf1970.cnif.cn/CN/10.13995/j.cnki.11-1802/ts.027163  或          http://sf1970.cnif.cn/CN/Y2021/V47/I24/77
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