Abstract: Probiotics can provide multiple health benefits for the host. In this study, twenty-two Lactobacillus strains and three Bifidobacterium strains were isolated from breast milk to screen strains with excellent probiotic properties. Seven strains were screened through acid and bile salt tolerance test, and subsequently the antibiotic sensitivity and the ability to inhibit pathogenic bacteria of these strains were determined. The results showed that all strains except strain M33 had good acid tolerance; Lactobacillus rhamnosus M53 and M60, Lactobacillus paracasei M71, Lactobacillus plantarum M113 and three Bifidobacterium strains exhibited good bile tolerance. Especially, bile tolerance of M53 and Bifidobacterium breve grx05 reached 45.72% and 75.54%, respectively. Strains M71 and grx05 were sensitive to eight kinds of antibiotics, and strains M53, M118 and S18 were sensitive to six kinds of antibiotics and had high safety; strain M53 was able to inhibit three Gram-positive bacteria and two Gram-negative bacteria, strain grx05 had obvious inhibitory effect on three Gram-positive bacteria and one Gram-negative bacteria. In summary, L. rhamnosus M53 and B. breve grx05 are promising for the development of fermented food or probiotic preparations.
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