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食品与发酵工业  2021, Vol. 47 Issue (14): 190-195    DOI: 10.13995/j.cnki.11-1802/ts.027231
  生产与科研应用 本期目录 | 过刊浏览 | 高级检索 |
母乳来源益生菌的筛选及潜在益生特性研究
胡鹏钰1,2, 于俊娟1,2, 王鹏1,2, 张臣臣1,2, 康文丽3, 戴智勇3, 汪家琦3, 潘丽娜3, 顾瑞霞1,2*
1(江苏省乳品生物技术与安全控制重点实验室(扬州大学), 江苏 扬州,225127)
2(扬州大学 食品科学与工程学院, 江苏 扬州,225127)
3(澳优乳业(中国)有限公司 澳优食品与营养研究院,湖南 长沙,410000)
Screening of probiotics from breast milk and its potential probiotic characteristics
HU Pengyu1,2, YU Junjuan1,2, WANG Peng1,2, ZHANG Chenchen1,2, KANG Wenli3, DAI Zhiyong3, WANG Jiaqi3, PAN Lina3, GU Ruixia1,2*
1(Jiangsu Key Laboratory of Dairy Biotechnology and Safety Control, Yangzhou University, Yangzhou 225127, China)
2(College of Food Science and Engineering, Yangzhou University, Yangzhou 225127, China)
3(Ausnutria Institute of Food and Nutrition, Ausnutria Dairy (China) Co. Ltd., Changsha 410000, China)
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摘要 益生菌能够维护肠道健康,为宿主提供多种健康益处。为了筛选出具有益生特性的优良菌株,该研究从母乳中分离筛选出22株乳杆菌和3株双歧杆菌。通过耐酸耐胆盐能力测试,筛选出7株菌株,并对其进行抗生素耐药性和致病菌抑制能力的测定。结果显示,除菌株M33外其余菌株都有较好的耐酸能力;鼠李糖乳杆菌M53和M60,副干酪乳杆菌M71,植物乳杆菌M113和3株双歧杆菌耐胆盐能力较好,其中M53和短双歧杆菌grx05耐胆盐能力较高,达到45.72%和75.54%;菌株M71和grx05对8种抗生素敏感,菌株M53,M118和S18对6种抗生素敏感,具有较高的安全性;菌株M53对3株革兰氏阳性菌和2株革兰氏阴性菌有明显的抑制效果,菌株grx05对3株革兰氏阳性菌和1株革兰氏阴性菌有明显的抑制效果。综合分析,鼠李糖乳杆菌M53和短双歧杆菌grx05有希望作为具有益生特性的菌株应用到发酵食品或益生菌制剂开发。
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胡鹏钰
于俊娟
王鹏
张臣臣
康文丽
戴智勇
汪家琦
潘丽娜
顾瑞霞
关键词:  母乳  益生菌  双歧杆菌  筛选  益生特性    
Abstract: Probiotics can provide multiple health benefits for the host. In this study, twenty-two Lactobacillus strains and three Bifidobacterium strains were isolated from breast milk to screen strains with excellent probiotic properties. Seven strains were screened through acid and bile salt tolerance test, and subsequently the antibiotic sensitivity and the ability to inhibit pathogenic bacteria of these strains were determined. The results showed that all strains except strain M33 had good acid tolerance; Lactobacillus rhamnosus M53 and M60, Lactobacillus paracasei M71, Lactobacillus plantarum M113 and three Bifidobacterium strains exhibited good bile tolerance. Especially, bile tolerance of M53 and Bifidobacterium breve grx05 reached 45.72% and 75.54%, respectively. Strains M71 and grx05 were sensitive to eight kinds of antibiotics, and strains M53, M118 and S18 were sensitive to six kinds of antibiotics and had high safety; strain M53 was able to inhibit three Gram-positive bacteria and two Gram-negative bacteria, strain grx05 had obvious inhibitory effect on three Gram-positive bacteria and one Gram-negative bacteria. In summary, L. rhamnosus M53 and B. breve grx05 are promising for the development of fermented food or probiotic preparations.
Key words:  breast milk    probiotics    Bifidobacterium    screening    probiotic property
收稿日期:  2021-03-10      修回日期:  2020-03-24           出版日期:  2021-07-25      发布日期:  2021-08-20      期的出版日期:  2021-07-25
基金资助: 湖南省创新平台与人才计划(2019TP2066);江苏省自然科学基金(BK20180910)
作者简介:  硕士研究生(顾瑞霞教授为通讯作者,E-mail:guruixia1963@163.com)
引用本文:    
胡鹏钰,于俊娟,王鹏,等. 母乳来源益生菌的筛选及潜在益生特性研究[J]. 食品与发酵工业, 2021, 47(14): 190-195.
HU Pengyu,YU Junjuan,WANG Peng,et al. Screening of probiotics from breast milk and its potential probiotic characteristics[J]. Food and Fermentation Industries, 2021, 47(14): 190-195.
链接本文:  
http://sf1970.cnif.cn/CN/10.13995/j.cnki.11-1802/ts.027231  或          http://sf1970.cnif.cn/CN/Y2021/V47/I14/190
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