Abstract: In order to improve the tenderness of puffer fish in the production and processing, puffer fish were used for the heat treatment at 50, 60 and 75 ℃ respectively. The effect of different heating temperatures on puffer fish tenderization was studied with shearing force as the main index, as well as cooking loss, water holding capacity, total texture, rheology and scanning electron microscope. The results showed that there were two absorption peaks in puffer fish without heat treatment by differential scanning calorimetry analysis, the fish treated at 50 ℃ and 60 ℃ had only one absorption peak, while the fish treated at 75 ℃ had no absorption peak which indicating that the protein had been completely denatured. With the increase of the treatment temperature, the cooking loss rate of fish meat gradually increased, and the water holding capacity first increased and then decreased. The elastic modulus and viscosity modulus of all heat-treated fish were higher than that of untreated fish, but with the increase of heating temperature, the elastic modulus and viscosity modulus of fish meat decreased gradually. The whole texture analysis showed that the chewiness, cohesiveness, resilience and adhesiveness of the heat-treated puffer fish were higher than those of the control group, and the shear stress of the fish was significantly reduced. The results of scanning electron microscopy showed that the muscle fibers of puffer fish after heat treatment became loose and then compact with the increase of heat treatment temperature. The tenderization effect of puffer fish was obvious when the heating temperature was 50 ℃, puffer fish had good tenderness and could maintain good meat quality.
吴炳存,尚珊,傅宝尚,等. 热处理对红鳍东方鲀鱼肉嫩化效果的影响[J]. 食品与发酵工业, 2021, 47(21): 202-209.
WU Bingcun,SHANG Shang,FU Baoshang,et al. Effect of heat treatment on the tenderization of Takifugu rubripes fish meat[J]. Food and Fermentation Industries, 2021, 47(21): 202-209.
[1] ZHOU R,ZHENG Y R,LIU Y,et al.Texture and quality assessment of ready-to-eat farmed obscure puffer fish (Takifugu obscurus) fillet by evaluating bacterial and myofibrillar degradation and biochemical changes during refrigerated storage[J].Journal of Aquatic Food Product Technology,2020,29(7):604-615. [2] LIAO Y,WANG W L,CHEN G L,et al.Basic taste characteristics of flavor material from cultured Takifugu obscurus by-products[J].Flavour and Fragrance Journal,2020,35(3):320-328. [3] 冯豪杰,蓝蔚青,刘大勇,等.不同减菌化处理方式对暗纹东方鲀冷藏期间品质变化的影响[J].食品科学,2020,41(7):210-217. FENG H J,LAN W Q,LIU D Y,et al.Effects of different sterilization treatments on the quality of puffer fish(Takifugu obscurus) during refrigerated storage[J].Food Science,2020,41(7):210-217. [4] 黄玉林,王文利,刘源,等.我国养殖河鲀(业)发展现状及研究进展[J].中国食品学报,2018,18(1):217-224. HUANG Y L,WANG W L,LIU Y,et al.The development and progress of cultured puffer fish in China[J].Journal of Chinese Institute of Food Science and Technology,2018,18(1):217-224. [5] PURSLOW P P.The structure and functional significance of variations in the connective tissue within muscle[J].Comparative Biochemistry and Physiology Part A:Molecular & Integrative Physiology,2002,133(4):947-966. [6] ROY B C,SEDGEWICK G,AALHUS J L,et al.Modification of mature non-reducible collagen cross-link concentrations in bovine m.gluteus Medius and semitendinosus with steer age at slaughter,breed cross and growth promotants[J].Meat Science,2015,110:109-117. [7] TAYLOR R G.Connective tissue structure function and influence on meat quality[J].Encyclopedia of Meat Science,2004(1):306-314. [8] WESTON A R,ROGERS R W,ALTHEN T G,et al.Review:the role of collagen in meat tenderness[J].The Professional Animal Scientist,2002,18(2):107-111. [9] 王选飞,刘俊荣,衣鸿莉,等.红鳍东方鲀肌肉组织及生化组成特性研究[J].食品科学技术学报,2020,38(6):93-102. WANG X F,LIU J R,YI H L,et al.Study on muscle texture and biochemical properties of farmed Takifugu rubripes [J].Journal of Food Science and Technology,2020,38(6):93-102. [10] GAO X,OGAWA H,TASHIRO Y,et al.Rheological properties and structural changes in raw and cooked abalone meat[J].Fisheries Science,2001,67(2):314-320. [11] 范三红,刘晓华,胡雅喃,等.加热处理对鱼肉蛋白质理化特性的影响[J].食品工业科技,2014,35(12):104-107;112. FAN S H,LIU X H,HU Y N,et al.Effect of heat treatment on protein in physical and chemical properties[J].Science and Technology of Food Industry,2014,35(12):104-107;112. [12] XU Y X,WANG R,ZHAO H L,et al.Effect of heat treatment duration on the interaction between fish myosin and selected flavor compounds[J].Journal of the Science of Food and Agriculture,2020,100(12):4 457-4 463. [13] 齐海萍,胡文忠,姜爱丽,等.热加工对鲤鱼质构的影响研究[J].食品科技,2011,36(5):144-146;150. QI H P,HU W Z,JIANG A L,et al.Effects of thermal processing on texture and protein of carp[J].Food Science and Technology,2011,36(5):144-146;150. [14] 桂萍,罗永康,冯力更.加热温度对混合肉肌原纤维蛋白质结构的影响[J].中国农业大学学报,2018,23(5):93-101. GUI P,LUO Y K,FENG L G.Effects of temperature on the structure of myofibrillar protein in blended meat[J].Journal of China Agricultural University,2018,23(5):93-101. [15] 邓丽,李岩,董秀萍,等.热加工过程中鲍鱼腹足蛋白间作用力及其质构特性[J].农业工程学报,2014,30(18):307-316. DENG L,LI Y,DONG X P,et al.Chemical interactions and textural characteristics of abalone pleopod muscle protein during heating[J].Transactions of the Chinese Society of Agricultural Engineering,2014,30(18):307-316. [16] BELIBAGLI K B,SPEERS R A,PAULSON A T.Thermophysical properties of silver Hake and mackerel surimi at cooking temperatures[J].Journal of Food Engineering,2003,60(4):439-448. [17] THIANSILAKUL Y,BENJAKUL S,RICHARDS M P.Isolation,characterisation and stability of myoglobin from Eastern little tuna (Euthynnus affinis) dark muscle[J].Food Chemistry,2011,124(1):254-261. [18] 姜启兴.鳙鱼肉热加工特性及其机理研究[D].无锡:江南大学,2015. JIANG Q X.Study on thermal processing properties and mechanism of bighead carp (Aristichthys nobilis) muscle[D].Wuxi:Jiangnan University,2015. [19] 朱瑞麒.淡水鱼鱼糜制品质量影响因素及其冷冻工艺优化[D].哈尔滨:东北农业大学,2015. ZHU R Q.Influence factors of quality of fresh water fish surimi products and optimization of freezing process[D].Harbin:Northeast Agricultural University,2015. [20] 尚珊,祁立波,姜鹏飞,等.海藻糖和复合磷酸盐对鲟鱼肉冻融稳定性的影响[J].食品工业科技,2020,41(9):205-209;326. SHANG S,QI L B,JIANG P F,et al.Effect of trehalose and composite phosphate on the freezing-thawing stability of sturgeon[J].Science and Technology of Food Industry,2020,41(9):205-209;326. [21] 鉏晓艳,邹开封,叶丽秀,等.嫩化时间对大口黑鲈半成品品质的影响[J].肉类研究,2016,30(7):1-5. ZU X Y,ZOU K F,YE L X,et al.Effect of tenderization time on the quality of semi-finished products from Micropterus salmoides[J].Meat Research,2016,30(7):1-5. [22] 鉏晓艳,邹开封,李新,等.鲌鱼肉制品嫩化工艺优化及表面微观结构观察[J].食品工业科技,2016,37(16):259-263;268. ZU X Y,ZOU K F,LI X,et al.Tenderizing process optimization and surface morphology observation on meat of Culter alburnus[J].Science and Technology of Food Industry,2016,37(16):259-263;268. [23] 夏俪宁.马鲛鱼肌原纤维蛋白氧化对凝胶特性影响[D].大连:大连工业大学,2018. XIA L N.The effect of oxidation on the gel properties of myofibrillar protein from mackerel(Scomberomours niphonius)[D].Dalian:Dalian University of Technology,2018. [24] 钱跃威, 徐瀚麟,吕奇晏,等.鳗鱼骨胶原肽钙螯合物的制备及其稳定性和Caco-2吸收特性[J].食品科学,2020,41(24):1-8. QIAN Y W,XU H L,LYU Q Y,et al.Preparation and stability of calcium-chelating peptide and its absorption characteristics in caco-2 cells[J].Food Science,2020,41(24):1-8. [25] 李超,徐为民,王道营,等.加热过程中肉嫩度变化的研究[J].食品科学,2009,30(11):262-265. LI C,XU W M,WANG D Y,et al.Heat induced change of meat tenderness[J].Food Science,2009,30(11):262-265. [26] PALKA K.Changes in intramuscular connective tissue and collagen solubility of bovine m.semitendinosus during retorting[J].Meat Science,1999,53(3):189-194. [27] 付湘晋.白鲢鱼脱腥及其低盐鱼糜制备的研究[D].无锡:江南大学,2009. FU X J.Removing the off-flavor of silver carp and the preparation of low salt surimi[D].Wuxi:Jiangnan University,2009. [28] 杨静.暗纹东方鲀冷藏过程中质构劣化和蛋白降解变化研究[D].无锡:江南大学,2017. YANG J.Study on texture softening and protein deterioration change of puffer fish(Takifugu obscurus) during refrigerated storage[D].Wuxi:Jiangnan University,2017. [29] 孙红霞,黄峰,丁振江,等.不同加热条件下牛肉嫩度和保水性的变化及机理[J].食品科学,2018,39(1):84-90. SUN H X,HUANG F,DING Z J,et al.Changes in tenderness and water-holding capacity and underlying mechanism during beef stewing[J].Food Science,2018,39(1):84-90. [30] UTAMA D T,BAEK K H,JEONG H S,et al.Effects of cooking method and final core-temperature on cooking loss,lipid oxidation,nucleotide-related compounds and aroma volatiles of Hanwoo brisket[J].Asian-Australasian Journal of Animal Sciences,2018,31(2):293-300. [31] 常海军.不同加工条件下牛肉肌内胶原蛋白特性变化及其对品质影响研究[D].南京:南京农业大学,2010. C H J.Study on the properties of intramuscular collagen in beef under different processing conditions and its effect on quality[D].Nanjing:Nanjing Agricultural University,2010. [32] 荣建华.冷冻和热加工对脆肉鲩肌肉特性的影响及其机制[D].武汉:华中农业大学,2015. RONG J H.Effects of freezing and hot processing on muscle characteristics of crisped grass carps and its mechanism[D].Wuhan:Huazhong Agricultural University,2015. [33] EGELANDSDAL B,MARTINSEN B,AUTIO K.Rheological parameters as predictors of protein functionality:A model study using myofibrils of different fibre-type composition[J].Meat Science,1995,39(1):97-111. [34] 姚志琴.鱼滑类预凝胶鱼糜制品的制备研究[D].杭州:浙江工业大学,2015. YAO Z Q.Study on preparation of fish surimi products with pre-gel of fish slime[D].Hangzhou:Zhejiang University of Technology,2015. [35] BECKER A,BOULAABA A,PINGEN S,et al.Low temperature cooking of pork meat - Physicochemical and sensory aspects[J].Meat Science,2016,118:82-88. [36] DAVEY C L,GILBERT K V.Temperature-dependant cooking toughness in beef[J].Journal of the Science of Food and Agriculture,1974,25(8):931-938. [37] KOPP J,BONNET M.Stress-strain and isometric tension measurements in collagen[M].New York:Van Nostrand,1987. [38] COMBES S,LEPETIT J,DARCHE B,et al.Effect of cooking temperature and cooking time on Warner-Bratzler tenderness measurement and collagen content in rabbit meat[J].Meat Science,2004,66(1):91-96. [39] 汪洋,王稳航.肌内结缔组织与肉的嫩度的关联机制及相关肉嫩化技术的研究进展[J/OL].食品科学,2020.http://kns.cnki.net/kcms/detail/11.2206.TS.20200722.1347.072.html. WANG Y,WANG W H.Research progress on the mechanism between intramuscular connective tissue and meat tenderness and related meat tenderization technology[J/OL].Food Science,2020.http:// http://kns.cnki.net/kcms/detail/11.2206.TS.20200722.1347.072.html. [40] CHRISTENSEN M,PURSLOW P P,LARSEN L M.The effect of cooking temperature on mechanical properties of whole meat,single muscle fibres and perimysial connective tissue[J].Meat Science,2000,55(3):301-307. [41] 臧大存,周光宏,徐幸莲,等.加热过程中鸭肉嫩度及超微结构的变化[J].江苏农业学报,2007,23(5):475-480. ZANG D C,ZHOU G H,XU X L,et al.Changes of ultra-structure and tenderness of duck meat during heating[J].Jiangsu Journal of Agricultural Sciences,2007,23(5):475-480. [42] 刘晶晶. 结缔组织热变化对牛肉嫩度影响的研究[D].北京:中国农业科学院,2018. LIU J J.Study on the effect of thermal change of connective tissue on beef tenderness[D].Beijing:Chinese Academy of Agricultural Sciences,2018. [43] 李艳红,王稳航.低温热处理对牦牛肉理化性质及感官特性的影响[J].食品与发酵工业,2021,47(2):145-152. LI Y H,WANG W H.Effects of low-temperature cooking on physicochemical properties and sensory of yak meat[J].Food and Fermentation Industries,201,47(2):145-152.