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食品与发酵工业  2021, Vol. 47 Issue (21): 225-230    DOI: 10.13995/j.cnki.11-1802/ts.027397
  分析与检测 本期目录 | 过刊浏览 | 高级检索 |
牛羊乳区别检验的PCR高分辨熔解检测方法
贺晓玲, 马西亚, 徐秦峰*
(陕西科技大学 食品与生物工程学院,陕西 西安,710021)
Identification of goat, sheep, bovine and water buffalo origin component in dairy products using high-resolution melting-PCR
HE Xiaoling, MA Xiya, XU Qinfeng*
(School of Food and Biological Engineering, Shaanxi University of Science and Technology, Xi’an 710021, China)
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摘要 乳源成分鉴别在乳制品掺假检测中具有重要意义。为了检测乳及其制品中牛、水牛、山羊、绵羊4种动物源性成分,建立了基于通用引物的PCR高分辨熔解曲线(high-resolution melting, HRM)检测方法。通过分析通用引物扩增子的熔解曲线,鉴别了含多种动物源性成分的混合物,检出了在山羊乳、水牛乳中掺入5%的牛乳,并准确判别市售乳及乳制品的乳源成分。该方法快速、灵敏、通量高,可以实现1对引物区分牛、水牛、山羊、绵羊4种动物源性成分,是一种有效的乳源成分鉴别方法,也可以用于检测肉及肉制品等其他含有4种以上动物源性成分的食品。
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贺晓玲
马西亚
徐秦峰
关键词:  牛乳  水牛乳  山羊乳  绵羊乳  通用引物  高分辨熔解曲线  掺假检测    
Abstract: The identification of the species origin is of great significance in dairy products adulteration detection. In order to detect four common animal species (bovine, buffalo, goat and sheep) in milk and dairy products, a PCR high-resolution melting (HRM) detection method was established based on universal primers detection. By analyzing the melting curve of the universal primer-generated amplicons, PCR-HRM was capable of identifying the mixtures of various animal-derived components, and also of sensitively detecting the presence of bovine milk in goat milk and buffalo milk down to 5%. In addition, it could accurately identify the species origin of commercial milk and dairy products. The proposed method is rapid, sensitive, high throughput, and can realize the identification of bovine, buffalo, goat and sheep with only one pair of primer. It is an effective method for the identification of milk derived constituents. It could be further applied to identify the above four animal species in various meat and meat products or other foods.
Key words:  bovine milk    buffalo milk    goat milk    sheep milk    universal primer    high-resolution melting    adulteration detection
收稿日期:  2021-03-22      修回日期:  2021-04-19           出版日期:  2021-11-15      发布日期:  2021-11-30      期的出版日期:  2021-11-15
基金资助: 国家自然科学基金项目(22074085);陕西省科技厅项目(2019 NY-126)
作者简介:  硕士研究生(徐秦峰教授为通讯作者,E-mail:xuqinfeng@sust.edu.cn)
引用本文:    
贺晓玲,马西亚,徐秦峰. 牛羊乳区别检验的PCR高分辨熔解检测方法[J]. 食品与发酵工业, 2021, 47(21): 225-230.
HE Xiaoling,MA Xiya,XU Qinfeng. Identification of goat, sheep, bovine and water buffalo origin component in dairy products using high-resolution melting-PCR[J]. Food and Fermentation Industries, 2021, 47(21): 225-230.
链接本文:  
http://sf1970.cnif.cn/CN/10.13995/j.cnki.11-1802/ts.027397  或          http://sf1970.cnif.cn/CN/Y2021/V47/I21/225
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