Purification, structural characterization and antioxidant activity of polysaccharide from the rhizome of Amomum villosum Lour
ZHOU Yang1, YANG Depo1,2, QIAN Chunguo1, HE Cuiqi1, ZHONG Jingtang3, XU Xinjun1,2, ZHAO Zhimin1,2*
1(School of Pharmaceutical Sciences, Sun Yat-Sen University, Guangzhou 510006, China) 2(Guangdong Technology Research Center for Advanced Chinese Medicine, Guangzhou 510006, China) 3(Yayisan Chinese Herbs Plantation Co.Ltd., Heyuan 517428, China)
Abstract: The rhizome of Amomum villosum was used as raw material to obtain the purified polysaccharide (AVRP-1), and the structural characteristics and antioxidant activities of AVRP-1 were further studied. DEAE-52 and Sephadex G-100 column chromatography were used to obtain AVRP-1. And the structure of AVRP-1 was characterized by chemical composition, high-performance gel permeation chromatography (HPGPC), HPLC and Fourier transform infrared (FT-IR) spectroscopy. The antioxidant activity of AVRP-1 was assessed based on the DPPH and hydroxyl radical scavenging rates in vitro. The results showed that the content of total sugar, protein and uronic acid of AVRP-1 was 90.34%, 0.64% and 43.59%, respectively. The relative molecular weight of AVRP-1 was 3 372.01 kDa. Moreover, AVRP-1 was composed of mannose, rhamnose, glucuronic acid, galacturonic acid, glucose, galactose and arabinose in the molar ratio of 4.14∶4.98∶5.10∶23.77∶17.30∶28.51∶16.19. The glycosidic bonds existed in both and configurations. The assay of antioxidant activity of AVRP-1 revealed that its half-maximal effective concentration (EC50) for DPPH and hydroxyl radical scavenging activity was 4.50 and 2.47 mg/mL, respectively. These results showed that AVRP-1 could be used as a natural antioxidant substance in the pharmaceutical and functional food industry, and provide a theoretical basis for making full use of A. villosum.
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2021, Vol. 47 
