1(College of Food Science, South China Agricultural University, Guangzhou 510642, China) 2(Space Science and Technology Institute, Shenzhen 518117, China)
Abstract: Exploring the variation of beer quality during storage and predict its shelf life is the basis of ensuring quality in Belgian ale amber craft beer. The original beer was firstly sterilized by ultra-high pressure (300 MPa, 15 min, 25℃) and simulated pasteurization (62℃ water bath, 30 min), respectively. Then the variation of total acid, diacetyl, colority, turbidity and foam stability of all beer samples during storage was recorded using accelerated shelf-life testing, and sensory evaluation was carried out to predict the shelf life of beer. The results showed that after the testing, the increments of total acid, diacetyl and colority of UHP samples were lower than those of the pasteurized samples, and the turbidity and foam stability were better, of which were closer to those of the original beer. The shelf life of UHP samples stored at 20 ℃ was 24 days, and that of the pasteurized samples was 7 days. The shelf life of UHP samples stored at 4 ℃ was 87 days, which was significantly higher than the 20 days of pasteurized samples. UHP sterilization combined with lower storage temperature can slow down the destruction of beer quality, better control the increase of diacetyl and total acid, as well as maintain good colority, turbidity and foam stability of beer. UHP treatment can not only kill harmful microbes of beer effectively, but also ensure the beer quality and prolong the shelf life.
张帆,蒋卓,张国文,等. 超高压杀菌对比利时艾尔琥珀工坊啤酒贮藏品质变化的影响及货架期预测[J]. 食品与发酵工业, 2021, 47(16): 203-210.
ZHANG Fan,JIANG Zhuo,ZHANG Guowen,et al. Effect of ultra high pressure sterilization on the storage quality and shelf life prediction of Belgian ale amber craft beer[J]. Food and Fermentation Industries, 2021, 47(16): 203-210.
[1] 金德强. 比利时风格强烈艾尔啤酒工艺研究[D].济南:齐鲁工业大学, 2019. JIN D Q.Study on the process of Belgian style strong ale beer[D].Jinan:Qilu University of Technology, 2019. [2] CHANADANG S, CHAMBERS IV E.Sensory shelf life estimation of novel fortified blended foods under accelerated and real-time storage conditions[J].Journal of Food Science, 2019, 84(9):2 638-2 645. [3] 胡华勇. 啤酒非生物稳定性的影响因素与控制措施[J].发酵科技通讯, 2020, 49(2):109-112. HU H Y.Influencing factors and control measures of beer non-biological stability of beer[J].Bulletin of Fermentation Science and Technology, 2020, 49(2):109-112. [4] 田玉红, 陈嵘, 杨朝霞, 等.啤酒货架期内特征风味的变化[J].中外酒业·啤酒科技, 2018(19):18-22. TIAN Y H, CHEN R, YANG Z X, et al.Changes in characteristic flavor of beer during shelf life[J].Global Alcinfo Beertech, 2018(19):18-22. [5] ZIELINSKI A, LECAPTAIN D.HPLC analysis for antioxidant properties of beer and the impact on shelf life[C].The American Chemical Society.New York, 2019. [6] 常斌, 蔡琳飞, 赵海锋.酿造过程对麦汁蛋白及其氧化特性的影响[J].现代食品科技, 2019, 35(12):145-150;40. CHANG B, CAI L F, ZHAO H F.Effect of brewing process on wort protein and its oxidative characteristics[J].Modern Food Science and Technology, 2019, 35(12):145-150;40. [7] KIM G C, KIM S B, KIM J S, et al.Changes in microbial growth, carotenoids, and water-soluble tannin content of ripe persimmon beverage after ultra-high pressure treatment[J].Food Science and Technology International, 2018, 24(4):351-360. [8] PHANCHAISRI C, CHAIKHAM P.Changes in lychee(Litchi chinensis Sonn.)texture and volatile compounds due to ultra-high pressure processing[J].International Food Research Journal, 2017, 24(3):1 220-1 226. [9] 赵晓丹, 刘夏衍, 陈芳, 等.超高压和高温短时杀菌对绿色复合果蔬汁的杀菌效果与品质影响[J].食品工业科技, 2019, 40(5):114-123. ZHAO X D, LIU X Y, CHEN F, et al.Effect of high hydrostatic pressure and high temperature short time treatment on the microbial and quality characteristics of green compound fruit and vegetable juice[J].Science and Technology of Food Industry, 2019, 40(5):114-123. [10] 迟恩忠, 王丽, 杜传来, 等.蓝莓原汁贮藏品质的变化及其货架期预测[J].食品工业, 2018, 39(2):187-190. CHI E Z, WANG L, DU C L, et al.Storage quality changes and the shelf life prediction of blueberry juice[J].The Food Industry, 2018, 39(2):187-190. [11] WANG J J, DING H J, ZHENG F Y, et al.Physiological changes of beer brewer′s yeast during serial beer fermentation[J].Journal of the American Society of Brewing Chemists, 2019, 77(1):10-20. [12] 杨静文, 刘泽畅, 陈培, 等.啤酒花中不同比例β-酸同系物的抗氧化及抑菌活性[J].食品科学, 2020, 41(23):83-90. YANG J W, LIU Z C, CHEN P, et al.Antioxidant and antibacterial activities of β-acid homologue mixtures with different ratios from hops[J].Food Science, 2020, 41(23):83-90. [13] 张洁. 新型酒花制品对啤酒相关微生物的影响研究[D].济南:齐鲁工业大学, 2020. ZHANG J.Effect of new hops extract on beer related microorganisms[D].Jinan:Qilu University of Technology, 2020. [14] TANIGUCHI Y, MATSUKURA Y, OZAKI H, et al.Identification and quantification of the oxidation products derived from α-acids and β-acids during storage of hops (Humulus lupulus L.)[J].Journal of Agricultural and Food Chemistry, 2013, 61(12):3 121-3 130. [15] 房慧婧, 李惠萍, 涂京霞, 等.特色啤酒的酿造和特点[J].啤酒科技, 2015(1):11-14. FANG H J, LI H P, TU J X, et al.The brewing and characteristics of specialty beer[J].Beer Science and Technology, 2015(1):11-14. [16] POCHITSKAYA I M, ROSLYAKOV Y F, LITVYAK V V, et al.Investigation of the influence of the melanoidin formation reaction on the content of amino acids in model food systems[J].Voprosy Pitaniia, 2018, 87(5):95-101. [17] FEGREDO J A, MEYNELL R, LAI A, et al.The Antioxidant Capacity of Beer:Relationships Between Assays of Antioxidant Capacity, Color and Other Alcoholic and Non-Alcoholic Beverages[M]//PREEDY V R. Beer in Health and disease prevention. Amsterdam:Elsevier, 2009:475-481. [18] GIBSON B, AUMALA V, HEINIO R L, et al.Differential evolution of Strecker and non-Strecker aldehydes during aging of pale and dark beers[J].Journal of Cereal Science, 2018, 83:130-138. [19] 郝秋娟, 尹长远.啤酒色泽研究进展[J].中国酿造, 2019, 38(11):6-10. HAO Q J, YIN C Y.Research progress on beer color[J].China Brewing, 2019, 38(11):6-10. [20] 武千钧, 林洪, 李梅, 等.温度和氧对啤酒老化的影响[J].食品与发酵工业, 2006, 32(4):1-4. WU Q J, LIN H, LI M, et al.Effects of temperature and oxygen on bottled beer aging during storage[J].Food and Fermentation Industries, 2006, 32(4):1-4. [21] JASKULA-GOIRIS B, DE CAUSMAECKER B, DE ROUCK G, et al.Influence of transport and storage conditions on beer quality and flavour stability[J].Journal of the Institute of Brewing, 2019, 125(1):60-68. [22] 张彦青, 李惠萍, 罗娜, 等.蛋白酶A对纯生啤酒泡持性的影响研究[J].中国酿造, 2016, 35(12):99-102. ZHANG Y Q, LI H P, LUO N, et al.Effect of proteinase A on draft beer foam stability[J].China Brewing, 2016, 35(12):99-102. [23] 黄蕴怡, 江国镜.不同发酵参数对酵母自溶性能的影响研究[J].中外酒业·啤酒科技, 2020(11):13-16. HUANG Y Y, JIANG G J.Study on the influence of different fermentation parameters on the autolysis performance of yeast[J].Global Alcinfo, 2020(11):13-16. [24] 杨铭, 郝晓娜, 罗天淇, 等.功能基因分析辅助筛选产双乙酰和乙偶姻乳酸菌[J].食品科学, 2020, 41(10):117-123. YANG M, HAO X N, LUO T Q, et al.Analysis of functional genes related to production of diacetal and acetoin by lactic acid bacteria[J].Food Science, 2020, 41(10):117-123. [25] BORCH-PEDERSEN K, MELLEGÅRD H, REINEKE K, et al.Effects of high pressure on Bacilluslicheniformis spore germination and inactivation[J].Applied and Environmental Microbiology, 2017, 83(14).DOI:10.1128/AEM.00503-17. [26] KIM S S, KIM S H, PARK S H, et al.Inactivation of Bacillus cereus spores on stainless steel by combined superheated steam and UV-C irradiation treatment[J].Journal of Food Protection, 2020, 83(1):13-16. [27] 汪浪红. 柚皮素协同脉冲电场杀灭大肠杆菌和金黄色葡萄球菌机制研究[D].广州:华南理工大学, 2019. WANG L H.Synergistic inactivation mechanism of Escherichia coli and Staphylococcus aureus by naringenin with pulsed electric fields[D].Guangzhou:South China University of Technology, 2019.
2021, Vol. 47 
