Effects of ethylene and 1-MCP on the content of cordycepin in Cordyceps militaris
SUN Qiang1*, MA Jinshuo2, LIAN Tiantian1, GUAN Ge1
1(School of Life Science and Technology, Sanquan College of Xinxiang Medical University, Xinxiang 453003, China) 2(School of Pharmacy, Sanquan College of Xinxiang Medical University, Xinxiang 453003, China)
Abstract: In this study, the effects of ethephon and ethylene receptor inhibitor 1-methylcyclopropene (1-MCP) on cordycepin production in the fruit body of Cordyceps militaris were studied. The fruit bodies were treated with ethylene, 1-MCP respectively. Real-time quantitative PCR was performed to detect the gene expression of adenyl-succinic acid synthase (Adss), adenyl-succinic acid lyase (Adsl), adenyl-kinase (AK) and purine nucleoside phosphorylase (PNP) among different groups. The content of cordycepin in the fruiting body was measured by HPLC. The results showed that the expression levels of PNP、Adss、AK and Adsl genes were significantly increased when the concentration of ethephon was 100 mg/L. The content of cordycepin in the ethylene group and control group were (25.37±0.22) μg/mL and (10.04±0.17) μg/mL respectively. And 1-MCP (1.0 mg/L) could significantly inhibit the cordycepin content in the fruit body and the expression of PNP、Adss、AK and Adsl genes. The cordycepin content in 1-MCP group was (0.52±0.12) μg/mL. In conclusion, the addition of exogenous ethylene at a certain concentration could promote the up-regulation of cordycepin metabolism-related gene expression, and the content of cordycepin in mature fruit bodies at a later stage was also significantly increased. Ethylene receptor inhibitors could significantly reduce the expression and content of cordycepin biosynthesis-related genes in the fruiting body.
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SUN Qiang,MA Jinshuo,LIAN Tiantian,et al. Effects of ethylene and 1-MCP on the content of cordycepin in Cordyceps militaris[J]. Food and Fermentation Industries, 2021, 47(21): 197-201.
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