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食品与发酵工业  2021, Vol. 47 Issue (21): 59-66    DOI: 10.13995/j.cnki.11-1802/ts.027982
  研究报告 本期目录 | 过刊浏览 | 高级检索 |
不同接种方式对青梅酒品质的影响及微生物多样性研究
刘英杰, 黄钧, 刘建, 吴重德, 金垚, 周荣清*
(四川大学 轻工科学与工程学院,四川 成都,610065)
Effect of different inoculation on the quality of greengage wines and microbial diversity
LIU Yingjie, HUANG Jun, LIU Jian, WU Chongde, JIN Yao, ZHOU Rongqing*
(College of Biomass Science and Engineering, Sichuan University, Chengdu 610065, China)
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摘要 以青梅为对象,采用多种检测方法探讨在不同接种方式(单培接种、共培接种、不接种)下发酵青梅酒的品质差异,开发了基于Saccharomyces cerevisiaeTorulaspora delbrueckii共培养的多轮补料发酵方式。实验结果显示,发酵提高了酒精含量和自由基清除能力,降低了总酸和挥发酸含量。不同属的2株酵母及顺序接种影响乙醇、代谢组分的含量,增高了醇类的比例、减少了酸类的比例,且显著减少了4-乙基苯酚、4-乙基愈创木酚和乙基酚等特征组分的含量。第2轮共培养发酵的萜烯类比例略增,其花香和果香更浓郁。强化发酵显著改变了酒的微生物群落结构。S.cerevisiaeT.delbrueckii的贡献类似,主要提高己酸乙酯、苯甲酸甲酯、乙酸异戊酯、异戊醇、橙花叔醇、法尼醇、癸醛等的含量。研究构建了一种新的青梅酒发酵方法。
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刘英杰
黄钧
刘建
吴重德
金垚
周荣清
关键词:  青梅酒  Saccharomyces cerevisiae  Torulaspora delbrueckii  风味  微生物多样性    
Abstract: The quality difference of greengage wines fermented by single culture inoculation, co-culture inoculation and no inoculation were studied. A fed-batch fermentation method based on Saccharomyces cerevisiae and Torulaspora delbrueckii co-culture was developed. The results showed that the content of alcohol and free radical scavenging ability were increased, the content of total and volatile acids were reduced, and the microbial community structure of wine was significantly changed by inoculating single culture and co-culturing. The proportion of alcohols was increased, the content of acids, 4-ethyl phenol, 4-ethylene glycol (4-EG) and ethylphenol were reduced by two genera strains with different inoculation. In the second batch of co-cultured fermentation, the proportion of terpenes increased slightly, floral and fruity aroma was stronger. S. cerevisiae and T. delbrueckii had similar contributions, mainly increased the content of ethyl caproate, methyl benzoate, isoamyl acetate, isoamyl alcohol, nerolidol, farnesol, decanal and so on. These results established a new fermentation method of greengage wine.
Key words:  greengage wine    Saccharomyces cerevisiae    Torulaspora delbrueckii    flavor    microbial diversity
收稿日期:  2021-05-08      修回日期:  2021-06-11           出版日期:  2021-11-15      发布日期:  2021-11-30      期的出版日期:  2021-11-15
基金资助: 四川省科技支撑计划项目(2016KJT0086)
作者简介:  硕士研究生(周荣清教授为通讯作者,E-mail:zhourqing@scu.edu.cn)
引用本文:    
刘英杰,黄钧,刘建,等. 不同接种方式对青梅酒品质的影响及微生物多样性研究[J]. 食品与发酵工业, 2021, 47(21): 59-66.
LIU Yingjie,HUANG Jun,LIU Jian,et al. Effect of different inoculation on the quality of greengage wines and microbial diversity[J]. Food and Fermentation Industries, 2021, 47(21): 59-66.
链接本文:  
http://sf1970.cnif.cn/CN/10.13995/j.cnki.11-1802/ts.027982  或          http://sf1970.cnif.cn/CN/Y2021/V47/I21/59
[1] 刘功德,苏艳兰,黄富宇,等.青梅的功能价值及加工研究进展[J].农业研究与应用,2018,31(4):1-8.
LIU G D,SU Y L,HUANG F Y,et al.Function value and processing research progress of Prunus mume[J].Agricultural Research and Application,2018,31(4):1-8.
[2] ADACHI M,SUZUKI Y,MIZUTA T,et al.The "Prunus mume Sieb.et Zucc" (Ume) is a rich natural source of novel anti-cancer substance[J].International Journal of Food Properties,2007,10(2):375-384.
[3] TIAN T T,SUN J Y,WU D H,et al.Objective measures of greengage wine quality:From taste-active compound and aroma-active compound to sensory profiles[J].Food Chemistry,2021,340:128179.
[4] HAN X Y,PENG Q,YANG H Y,et al.Influence of different carbohydrate sources on physicochemical properties and metabolites of fermented greengage (Prunus mume) wines[J].LWT-Food Science and Technology,2020,121:108 929.
[5] SADOUDI M,TOURDOT-MARÉCHAL R,ROUSSEAUX S,et al.Yeast-yeast interactions revealed by aromatic profile analysis of Sauvignon Blanc wine fermented by single or co-culture of non-Saccharomyces and Saccharomyces yeasts[J].Food Microbiology,2012,32(2):243-253.
[6] TOFALO R,PATRIGNANI F,LANCIOTTI R,et al.Aroma profile of montepulciano d’Abruzzo wine fermented by single and co-culture starters of autochthonous Saccharomyces and non-Saccharomyces yeasts[J].Frontiers in Microbiology,2016,7:610.
[7] LIU J,LIU M,YE P,et al.Characterization of major properties and aroma profile of kiwi wine co-cultured by Saccharomyces yeast (S.cerevisiae,S.bayanus,S.uvarum) and T.delbrueckii[J].European Food Research and Technology,2020,246(4):807-820.
[8] 丁莹, 李亚辉,蒲青,等.我国果酒行业发展现状及前景分析[J].酿酒科技,2019(4):104-107.
DING Y,LI Y H,PU Q,et al.Analysis of the present status and development prospects of fruit wine industry in china[J].Liquor-Making Science & Technology,2019(4):104-107.
[9] CHEN D,YAP Z Y,LIU S Q,et al.Evaluation of the performance of Torulaspora delbrueckii,Williopsis saturnus,and Kluyveromyces lactis in lychee wine fermentation[J].International Journal of Food Microbiology,2015,206:45-50.
[10] BENITO S.The impact of Torulaspora delbrueckii yeast in winemaking[J].Applied Microbiology and Biotechnology,2018,102(7):3 081-3 094.
[11] BIMPILAS A,PANAGOPOULOU M,TSIMOGIANNIS D,et al.Anthocyanin copigmentation and color of wine:The effect of naturally obtained hydroxycinnamic acids as cofactors[J].Food Chemistry,2016,197(Pt A):39-46.
[12] SINGLETON,V.L.,ROSSI,et al.Colorimetry of total phenolics with phosphomolybdic-phosphotungstic acid reagents[J].American Journal of Enology and Viticulture,1965,16(3):144-158.
[13] WANG X,XIE K L,ZHUANG H N,et al.Volatile flavor compounds,total polyphenolic contents and antioxidant activities of a China gingko wine[J].Food Chemistry,2015,182:41-46.
[14] ZHANG L Q,HUANG J,ZHOU R Q,et al.Evaluating the feasibility of fermentation starter inoculated with Bacillus amyloliquefaciens for improving acetoin and tetramethylpyrazine in Baoning bran vinegar[J].International Journal of Food Microbiology,2017,255:42-50.
[15] ZHENG J,LIANG R,WU D C,et al.Discrimination of different kinds of Luzhou-flavor raw liquors based on their volatile features[J].Food Research International,2014,56:77-84.
[16] NIU M C,HUANG J,JIN Y,et al.Volatiles and antioxidant activity of fermented goji (Lycium Chinese) wine:Effect of different oak matrix (barrel,shavings and chips)[J].International Journal of Food Properties,2017,20(sup2):2 057-2 069.
[17] HE G Q,DONG Y,HUANG J,et al.Alteration of microbial community for improving flavor character of Daqu by inoculation with Bacillus velezensis and Bacillus subtilis[J].LWT-Food Science and Technology,2019,111:1-8.
[18] TANG Q X,HE G Q,HUANG J,et al.Characterizing relationship of microbial diversity and metabolite in Sichuan Xiaoqu[J].Frontiers in Microbiology,2019,10:696.
[19] TIAN T T,WU D H,NG C T,et al.A multiple-step strategy for screening Saccharomyces cerevisiae strains with improved acid tolerance and aroma profiles[J].Applied Microbiology and Biotechnology,2020,104(7):3 097-3 107.
[20] COJOCARU G A,ANTOCE A O.Influence of glutathione and ascorbic acid treatments during vinification of feteasca regala variety and their antioxidant effect on volatile profile[J].Biosensors,2019,9(4):140.
[21] BERENGUER M,VEGARA S,BARRAJÓN E,et al.Physicochemical characterization of pomegranate wines fermented with three different Saccharomyces cerevisiae yeast strains[J].Food Chemistry,2016,190:848-855.
[22] BALMASEDA A,ROZÈS N,LEAL M Á,et al.Impact of changes in wine composition produced by non-Saccharomyces on malolactic fermentation[J].International Journal of Food Microbiology,2021,337:108954.
[23] 熊勤梅, 肖胜舰,黄钧等.发酵型青梅酒的微滤:膜污染分析与品质评价[J].食品与发酵工业,2020,46(15):77-83.
XIONG Q M,XIAO S J,HUANG J,et al.Microfiltration of greengage wine:Membrane fouling analysis and quality evaluation[J].Food and Fermentation Industries,2020,46(15):77-83.
[24] TIAN T T,YANG H,YANG F,et al.Optimization of fermentation conditions and comparison of flavor compounds for three fermented greengage wines[J].LWT - Food Science and Technology,2018,89:542-550.
[25] 杨红亚, 吴少华,王兴红,等.气质联用分析青梅发酵酒和浸泡酒的香气成分[J].酿酒科技,2005(9):80-83.
YANG H Y,WU S H,WANG X H,et al.Analysis of flavoring compositions in green plum fruit fermenting wine and in green plum fruit steeping wine by GC-MS[J].Liquor-Making Science & Technology,2005(9):80-83.
[26] ZHENG X H,ZHANG M,FANG Z X,et al.Effects of low frequency ultrasonic treatment on the maturation of steeped greengage wine[J].Food Chemistry,2014,162:264-269.
[27] KHEIR J,SALAMEH D,STREHAIANO P,et al.Impact of volatile phenols and their precursors on wine quality and control measures of Brettanomyces/Dekkera yeasts[J].European Food Research and Technology,2013,237(5):655-671.
[28] CHATONNET P,DUBOURDIE D,BOIDRON,et al.The origin of ethylphenols in wines[J].Journal of the Science of Food and Agriculture,1992,60(2):165-178.
[29] HU B R,CAO Y,ZHU J Y,et al.Analysis of metabolites in chardonnay dry white wine with various inactive yeasts by 1H NMR spectroscopy combined with pattern recognition analysis[J].AMB Express,2019,9(1):140.
[30] RÍOS-REINA R,SEGURA-BORREGO M P,GARCÍA-GONZÁLEZ D L,et al.A comparative study of the volatile profile of wine vinegars with protected designation of origin by headspace stir bar sorptive extraction[J].Food Research International,2019,123:298-310.
[31] GROSCH W.Evaluation of the key odorants of foods by dilution experiments,aroma models and omission[J].Chemical Senses,2001,26(5):533-545.
[32] BÖHMER M,SMOL’AK D,ŽENIŠOVÁ K,et al.Comparison of microbial diversity during two different wine fermentation processes[J].FEMS Microbiology Letters,2020,367(18):fnaa150.
[33] KONG C L,LI A H,SU J,et al.Flavor modification of dry red wine from Chinese spine grape by mixed fermentation with Pichia fermentans and S.cerevisiae[J].LWT-Food Science and Technology,2019,109:83-92.
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