Abstract: The six highland barley varieties from Tibet, including Guoluo, Himalaya 22, Himalaya 19, Dongqing 1, Zangqing 2000 and Zangqing 690, were used as materials to investigate the accumulation of phenolics in different varieties of germinated highland barley (Qingke) and the germination conditions on the synthesis of phenolics in order to obtain the suitable varieties and germination conditions for the phenolics accumulation. After germination at 30 ℃ for 36 h, the physiological and biochemical indexes, phenolics content and the key enzyme activities were determined to screen the varieties. Then, the effects of germination time and temperature on the content of total phenolics were studied. Among the six tested varieties, although the germination rate of Dongqing 1 was low, the respiration rate and fresh weight were the highest which indicated that the metabolism of Dongqing 1 was vigorous. At the same time, the content of total phenolics and phenolic acids was the highest, and the activity of phenolics synthetase was relatively higher. Therefore, Dongqing 1 was selected as the suitable highland barley variety for accumulating phenolics by germination. During 24-72 h of germination, the content of free phenolics decreased, but the difference of total phenolics content among the germination time points was not significant. The lower the temperature (25-30 ℃), the more conducive to the accumulation of phenolics. The results of response surface optimization showed that the total phenolics content of highland barley reached 296.72 mg GAE/100g DW after germination at 25 ℃ for 48 h, which was 1.47 times of the raw material (201.62 mg GAE/100g DW). The optimal germination condition of Dongqing 1 highland barley was 25 ℃ for 48 h, and the content of phenolics was the highest. This study provided theoretical and data support for the development of Tibetan pre-germinated highland barley rich in phenolics and its functional products.
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2022, Vol. 48 
