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食品与发酵工业  2021, Vol. 47 Issue (21): 86-93    DOI: 10.13995/j.cnki.11-1802/ts.028751
  研究报告 本期目录 | 过刊浏览 | 高级检索 |
同时降解两种真菌毒素的食品级重组酵母培养条件优化
夏雨1*, 吴梓凤1, 胡修玉2, 包红朵3, 何瑞1, 秋杨煜1, 程倩倩1, 王周平1
1(江南大学 食品学院,江苏 无锡,214122)
2(中国生物发酵产业协会,北京,100833)
3(江苏省农业科学院 农产品质量安全与营养研究所,江苏 南京,210014)
Optimal cultivation conditions for food-grade recombinant yeast that simultaneously degrades two mycotoxins
XIA Yu1*, WU Zifeng1, HU Xiuyu2, BAO Hongduo3, HE Rui1, QIU Yangyu1, CHENG Qianqian1, WANG Zhouping1
1(School of Food Science and Technology, Jiangnan University, Wuxi 214122, China)
2(China Biotech Fermentation Industry Association, Beijing 100833, China)
3(Institute of Food Safety and Nutrition, Jiangsu Academy of Agricultural Sciences, Nanjing 210014, China)
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摘要 为提高能同时降解黄曲霉毒素B1和玉米赤霉烯酮的食品级重组乳酸克鲁维酵母GG799(pKLAC1-ZPF1)的培养生物量,对该菌进行了高密度培养条件优化。选择并试验了几种基础培养基的摇瓶培养效果,在此基础上采用单因素试验和响应面试验对该菌的培养基成分进行优化,采用单因素试验和正交试验对该菌的培养工艺参数进行优化,最后以5 L发酵罐扩大培养来验证优化结果。结果表明摇瓶优化的培养基成分为:葡萄糖20.41 g/L、蛋白胨30.46 g/L、酵母提取物24.73 g/L、KH2PO4 4.48 g/L、MgSO4 0.65 g/L,在此条件下获得生物量是对照的5.5倍;优化的培养参数为:培养温度26 ℃、转速220 r/min、初始pH 4.5,在此条件下获得生物量是对照的7.5倍。按照优化的培养基和培养参数进行发酵罐扩大培养,获得的生物量为(34.05±3.34) g/L,是摇瓶培养对照的29.6倍。研究结果为食品级重组乳酸克鲁维酵母工业化应用提供了重要理论依据和参考数据。
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夏雨
吴梓凤
胡修玉
包红朵
何瑞
秋杨煜
程倩倩
王周平
关键词:  食品级重组酵母  乳酸克鲁维酵母  培养条件优化  黄曲霉毒素B1  玉米赤霉烯酮    
Abstract: To improve the biomass of recombinant Kluyveromyces lactis GG799(pKLAC1-ZPF1), the optimal high-density cultivation conditions was conducted. The optimal conditions were verified in a 5 L fermentor. The results showed that the optimized culture composition was as follows: 20.41 g/L of glucose, 30.46 g/L of peptone, 24.73 g/L of yeast extract, 4.48 g/L of KH2PO4 and 0.65 g/L of MgSO4, and the biomass was 5.5 times of control. While the optimized cultivation parameters were as follows: 26℃, 220 r/min and the initial pH 4.5, and the biomass were 7.5 times of control. The biomass was (34.05±3.34) g/L in 5 L fermentor, which was 29.6 times of control. The results provided important theoretical basis and reference data for the future industrial production of K. lactis.
Key words:  food-grade recombinant yeast    Kluyveromyces lactis    optimization of culture conditions    aflatoxin B1    zearalenone
收稿日期:  2021-07-22      修回日期:  2021-08-03           出版日期:  2021-11-15      发布日期:  2021-11-30      期的出版日期:  2021-11-15
基金资助: 江苏省农业科技自主创新资金项目(CX(19)3109)
作者简介:  博士,副教授(通讯作者,E-mail:yuxia@jiangnan.edu.cn)
引用本文:    
夏雨,吴梓凤,胡修玉,等. 同时降解两种真菌毒素的食品级重组酵母培养条件优化[J]. 食品与发酵工业, 2021, 47(21): 86-93.
XIA Yu,WU Zifeng,HU Xiuyu,et al. Optimal cultivation conditions for food-grade recombinant yeast that simultaneously degrades two mycotoxins[J]. Food and Fermentation Industries, 2021, 47(21): 86-93.
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http://sf1970.cnif.cn/CN/10.13995/j.cnki.11-1802/ts.028751  或          http://sf1970.cnif.cn/CN/Y2021/V47/I21/86
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