Please wait a minute...
 
 
食品与发酵工业  2022, Vol. 48 Issue (15): 98-101    DOI: 10.13995/j.cnki.11-1802/ts.029859
  研究报告 本期目录 | 过刊浏览 | 高级检索 |
益生菌饮料改善甲型副伤寒沙门氏菌诱导小鼠腹泻作用研究
薛玉玲1,2, 袁庆彬1,2*, 冯丽莉1,2, 张栋1,2, 康志远1,2, 王世杰1,3*
1(石家庄君乐宝乳业有限公司,河北 石家庄,050221)
2(河北省发酵乳制品技术创新中心,河北 石家庄,050221)
3(河北科技大学 生物科学与工程学院,河北 石家庄,050018)
Effect of probiotics beverage on relieving diarrhea induced by Salmonella paratyphi A in mice
XUE Yuling1,2, YUAN Qingbin1,2*, FENG Lili1,2, ZHANG Dong1,2, KANG Zhiyuan1,2, WANG Shijie1,3*
1(Shijiazhuang Junlebao Dairy Co. Ltd., Shijiazhuang 050221, China)
2(Hebei Fermented Dairy Products Technological Innovation Center, Shijiazhuang 050221, China)
3(College of Biological Sciences and Engineering, Hebei University of Science and Technology, Shijiazhuang 050018, China)
下载:  HTML  PDF (786KB) 
输出:  BibTeX | EndNote (RIS)      
摘要 考察嗜酸乳杆菌和动物双歧杆菌乳亚种益生菌饮料对甲型副伤寒沙门氏菌诱导的腹泻改善效果,并探究其作用机制。饲喂腹泻模型小鼠益生菌饮料原液,观察益生菌饮料对腹泻改善情况;饲喂正常小鼠沙门氏菌和益生菌饮料混合液,观察小鼠腹泻情况,检测粪便中的乳杆菌和双歧杆菌的数量,探究益生菌的竞争抑制作用;饲喂正常小鼠益生菌饮料以及菌粉,检测粪便及胃肠道组织内的乳杆菌和双歧杆菌的数量,探究益生菌的定植作用。结果显示,饲喂益生菌饮料原液的实验组腹泻模型小鼠1 d后腹泻停止,较对照小组恢复快;饲喂沙门氏菌和益生菌饮料混合液,小鼠没有出现水样便和腹泻,且5 d后的粪便中益生菌数量与对照组相近;小鼠肠道内的乳杆菌和双歧杆菌的数目均随着饲喂益生菌饮料以及菌粉时间的延长呈上升趋势。综上,益生菌饮料基于益生菌可通过竞争抑制、定植肠道等作用,改善由甲型副伤寒沙门氏菌引起的小鼠腹泻症状,对肠道起到保护作用。
服务
把本文推荐给朋友
加入引用管理器
E-mail Alert
RSS
作者相关文章
薛玉玲
袁庆彬
冯丽莉
张栋
康志远
王世杰
关键词:  益生菌  腹泻  甲型副伤寒沙门氏菌  肠道菌群  定植    
Abstract: To discover the effect of probiotic beverage on relieving diarrhea induced by Salmonella paratyphi A and explore its mechanism, the diarrhea model mice were fed with probiotic beverage fermented by Lactobacillus acidophilus and Bifidobacterium animalis subsp. lactis. The diarrhea occurrence of mice was observed, and the count of Lactobacillus acidophilus and B. animalis subsp. lactis in feces were detected. The normal mice fed with the probiotics beverage and powder were intended to explore whether the probiotics have intestinal colonization effect. The count of L. acidophilus and B. animalis subsp. lactis in feces and intestinal tract were detected. The results showed that the diarrhea model mice in the experimental group fed with probiotic beverage stopped diarrhea after 1 d, and recovered faster than those in the control group. No watery stools and diarrhea showed up in mice which were fed with S. paratyphi A and probiotics beverage. The count of probiotics in feces after 5 d was similar to the control group. The amount of Lactobacillus acidophilus and B. animalis subsp. lactis in the intestinal tract of mice increased with the feeding time. The results suggested that by means of probiotic competition inhibition and colonization of the intestinal tract, probiotic beverage can relieve the diarrhea symptoms caused by S. paratyphi A in mice, and protect the intestinal tract as well.
Key words:  probiotics    diarrhea    Salmonella paratyphi A    gut microbiota    colonization
收稿日期:  2021-10-27      修回日期:  2021-12-24           出版日期:  2022-08-15      发布日期:  2022-09-02      期的出版日期:  2022-08-15
基金资助: 河北省省级科技计划资助项目(19027143Z,19227136D,199676161H);石家庄科学技术研究与发展计划资助项目(201170154A)
作者简介:  第一作者:硕士,工程师(王世杰高级工程师和袁庆彬博士研究生为共同通信作者,E-mail:mrshjwang@163.com;yuanqingbin@jlbry.cn)
引用本文:    
薛玉玲,袁庆彬,冯丽莉,等. 益生菌饮料改善甲型副伤寒沙门氏菌诱导小鼠腹泻作用研究[J]. 食品与发酵工业, 2022, 48(15): 98-101.
XUE Yuling,YUAN Qingbin,FENG Lili,et al. Effect of probiotics beverage on relieving diarrhea induced by Salmonella paratyphi A in mice[J]. Food and Fermentation Industries, 2022, 48(15): 98-101.
链接本文:  
http://sf1970.cnif.cn/CN/10.13995/j.cnki.11-1802/ts.029859  或          http://sf1970.cnif.cn/CN/Y2022/V48/I15/98
[1] CRUMP J A. Progress in typhoid fever epidemiology[J]. Clinical Infectious Diseases, 2019(Supplement_1): S4-S9.
[2] 卫生部疾病预防控制局. 伤寒、副伤寒防治手册[M]. 北京: 人民卫生出版社, 2006.Department of Disease Control and Prevention, Ministry of Health. Handbook on the prevention and treatment of typhoid and paratyphoid[M]. Beijing: People’s Medical Publishing House, 2006.
[3] SETHI S, GAUTAM V, GUPTA K, et al. Vertical transmission of Salmonella enterica serotype Paratyphi A leading to abortion[J]. JMM Case Reports, 2017, 4(11): e005127.
[4] GIBANI M M, BRITTO C, POLLARD A J. Typhoid and paratyphoid fever: A call to action[J]. Current Opinion in Infectious Diseases, 2018, 31(5): 440-448.
[5] TACCONELLI E, CARRARA E, SAVOLDI A, et al. Discovery, research, and development of new antibiotics: The WHO priority list of antibiotic-resistant bacteria and tuberculosis[J]. The Lancet Infectious Diseases, 2018, 18(3):318-327.
[6] SLATTERY C, COTTER P D, O’TOOLE P W. Analysis of health benefits conferred by Lactobacillus species from kefir[J]. Nutrients, 2019, 11(6): 1252.
[7] 李新秋, 佘勇, 陈军, 等. 益生菌对抗菌素致肠道菌群紊乱患者免疫指标及炎症指标的影响[J]. 航空航天医学杂志, 2020, 31(9): 1 091-1 092.LI X Q, SHE Y, CHEN J, et al. Effects of probiotics on immune and inflammatory indexes in patients with intestinal flora disorder caused by antibiotics[J]. Journal of Aerospace Medicine, 2020, 31(9): 1 091-1 092.
[8] 吴炜倩, 陆合, 郭亚楠, 等. 益生菌防治过敏性疾病的进展和展望[J].中国微生态学杂志, 2020, 32(7): 862-865.WU W Q, LU H, GUO Y N, et al. Progress and prospect of probiotics in the prevention and treatment of allergic diseases[J]. Chinese Journal of Microecology, 2020, 32(7): 862-865.
[9] 伍梓汐, 李姗珊, 沈曦, 等. 益生菌免疫调节功能研究[J].食品科技, 2017, 42(1): 29-33.WU Z X, LI S S, SHEN X, et al. Effects on immunomodulatory of probiotics[J]. Food Science and Technology, 2017, 42(1):29-33.
[10] KEMGANG T S, KAPILA S, SHANMUGAM V P, et al. Cross-talk between probiotic Lactobacilli and host immune system[J]. Journal of Applied Microbiology, 2014, 117(2):303-319.
[11] LIN S Y, AYRES J W, WINKLER W JR, et al. Lactobacillus effects on cholesterol: In vitro and in vivo results[J]. Journal of Dairy Science, 1989, 72(11):2 885-2 899.
[12] 王志强, 马丽娟, 周海静, 等. 益生菌抗肿瘤作用及其机制的研究进展[J]. 国际口腔医学杂志, 2017, 44(6):636-641.WANG Z Q, MA L J, ZHOU H J, et al. Research progress on antitumor effect and mechanism of probiotics[J]. International Journal of Stomatology, 2017, 44(6):636-641.
[13] 阿热爱·巴合提, 武瑞赟, 肖梦圆,等. 益生菌的生理功能及作用机理研究进展[J]. 食品与发酵工业, 2020, 46(22): 270-275.AREAI B, WU R Y, XIAO M Y, et al. Research progress in physiological function and mechanism of probiotics[J]. Food and Fermentation Industries, 2020, 46(22): 270-275.
[14] 赵梓雯, 杨虹. 短双歧杆菌对鼠伤寒沙门氏菌的抑制[J]. 微生物学通报, 2019, 46(10): 2 673-2 688.ZHAO Z W, YANG H. Inhibition of Salmonella typhimurium by Bifidobacterium breve[J]. Microbiology China, 2019, 46(10):2 673-2 688.
[15] KEMGANG T S, KAPILA S, SHANMUGAM V P, et al. Fermented milk with probiotic Lactobacillus rhamnosus S1K3 (MTCC5957) protects mice from Salmonella by enhancing immune and nonimmune protection mechanisms at intestinal mucosal level[J]. The Journal of Nutritional Biochemistry, 2016, 30: 62-73.
[16] 林如琴, 吴娴波, 张益多, 等. 嗜酸乳杆菌抑制大肠杆菌O157:H7肠道定植体内外实验[J]. 实用医学杂志, 2016, 32(16): 2 603-2 607. LIN R Q, WU X B, ZHANG Y D, et al. Inhibiting effect of Lactobacillus acidophilus on intestinal colonization of enterohaemorrhagic E.coli O157:H7 in vitro and vivo[J]. The Journal of Practical Medicine, 2016, 32(16): 2 603-2 607.
[17] JUNGERSEN M, WIND A, JOHANSEN E, et al. The science behind the probiotic strain Bifidobacterium animalis subsp. lactis BB-12®[J]. Microorganisms, 2014, 2(2):92-110.
[18] GOPAL P K, PRASAD J, SMART J, et al. In vitro adherence properties of Lactobacillus rhamnosus DR20 and Bifidobacterium lactis DR10 strains and their antagonistic activity against an enterotoxigenic Escherichia coli[J]. International Journal of Food Microbiology, 2001, 67(3):207-216.
[19] 王梦颖, 赵国忠, 韩俊燕, 等. 食源性乳酸菌的分离及其在小鼠肠道的定植能力[J]. 中国食品学报, 2018, 18(5): 239-245.WANG M Y, ZHAO G Z, HAN J Y, et al. Isolation of foodborne lactic acid bacteria and detection of the colonization ability in mouse intestinal tract[J]. Journal of Chinese Institute of Food Science and Technology, 2018, 18(5): 239-245.
[1] 李洁, 鲁皓, 刘柳, 吴晓霞, 李成, 丁聪, 李建科. 益生菌发酵豆乳对提高结合态大豆异黄酮转化的效果[J]. 食品与发酵工业, 2022, 48(9): 91-96.
[2] 杨琦, 谢纯良, 周映君, 龚文兵, 朱作华, 许超, 彭源德. 益生菌发酵蓝莓的体外抗氧化活性分析[J]. 食品与发酵工业, 2022, 48(9): 112-116.
[3] 周立光, 杨明喆, 冯会粉, 刘艺茹, 刘蕊, 张欣, 葛媛媛, 张旭光, 刘佳奇, 程坤, 于学健, 姚粟. 复合益生菌产品菌种鉴定及活菌定量检测方法[J]. 食品与发酵工业, 2022, 48(9): 235-244.
[4] 祁亚劲, 贺禹丰, 杨羽倩, 石刘婷, 詹语, 鲁佳欣, 郭敏, 王慧玲, 赵建新, 张灏, 王刚, 陈卫. 植物乳杆菌发酵豆乳对多囊卵巢综合征大鼠肠道菌群紊乱的改善作用[J]. 食品与发酵工业, 2022, 48(8): 90-97.
[5] 王超, 高磊, 赵子健, 赵玉娟, 杨舸, 牛春华, 李盛钰. 益生菌附属发酵剂对切达干酪质构、游离氨基酸和短链脂肪酸的影响[J]. 食品与发酵工业, 2022, 48(8): 136-142.
[6] 李雅丽, 王默涵, 赵雯, 段素芳, 刘伟贤, 陈萌, 刘义凤, 段盛林, 洪维鍊. 益生菌与益生元组合的筛选及体外发酵特性研究[J]. 食品与发酵工业, 2022, 48(7): 50-57.
[7] 张玉双, 代安娜, 丁功涛, 罗丽, 王宝民, 刘红娜, 丁波. 牦牛乳与豆乳比例、益生菌和浓缩果汁种类对发酵饮料品质的影响[J]. 食品与发酵工业, 2022, 48(7): 97-102.
[8] 曾献春, 朱雅琴, 王明明, 李佳雯, 刘蔓萩, 庄伟伟. 干酪乳杆菌对便秘小鼠肠道菌群的影响[J]. 食品与发酵工业, 2022, 48(6): 64-70.
[9] 胡博, 陈雪梅, 唐晓姝, 张白曦, 郭自涛. 基于高通量测序分析红枣枸杞浸泡酒对小鼠肠道菌群的影响[J]. 食品与发酵工业, 2022, 48(5): 47-52.
[10] 王琳琳, 杨树荣, 王嘉良, 王刚, 张灏, 赵建新, 陈卫. 副干酪乳杆菌LPC-F通过促进Cajal间质细胞增殖缓解便秘[J]. 食品与发酵工业, 2022, 48(4): 1-9.
[11] 唐史杰, 陈敏, 尹明雨. 醋酸菌调控肠道菌群以解酒护肝的研究进展[J]. 食品与发酵工业, 2022, 48(4): 301-306.
[12] 朱广素, 赵建新, 张灏, 陈卫, 王刚. 短双歧杆菌对Aβ1-42导致的阿尔兹海默症小鼠肠道菌群及代谢物的影响[J]. 食品与发酵工业, 2022, 48(3): 70-77.
[13] 吴影, 古绍彬, 吴昊天, 周艳林, 赵丽娜, 李欣, 李璇, 郭金英. 复合益生菌剂量对小鼠生理特性和肠道菌群的影响[J]. 食品与发酵工业, 2022, 48(3): 78-84.
[14] 王茜茜, 张婷, 梁明, 赵建新, 张灏, 王刚, 陈卫. 罗伊氏乳杆菌CCFM8631对由胆碱导致的血浆氧化三甲胺和盲肠三甲胺水平升高的影响[J]. 食品与发酵工业, 2022, 48(16): 11-17.
[15] 田培郡, 蒋娇娜, 吕天凤, 王荣, 王刚, 陈卫. 靶向肠道菌群改善失眠的研究进展及展望[J]. 食品与发酵工业, 2022, 48(15): 303-308.
[1] ZHANG Dong et al. Effect of different amounts of salt on quality of bacon[J]. Food and Fermentation Industries, 2017, 43(11): 159 .
[2] WANG An-feng et al. Optimization of hydrolysis process of pinctadafucata by response surface method[J]. Food and Fermentation Industries, 2017, 43(11): 165 .
[3] WU Peng et al. The development of microwave cooked carrot chips based on domestic microwave oven[J]. Food and Fermentation Industries, 2017, 43(11): 180 .
[4] WANG Jun et al. Screening methodof37 foodadditivesinfruitcansby UPLC-Q/TOF[J]. Food and Fermentation Industries, 2017, 43(11): 212 .
[5] . Isolation and identification of anaerobic bacteria in the process of Maotai-flavor liquor brewing[J]. Food and Fermentation Industries, 0, (): 1 .
[6] YU Qing-lin et al. Fermentation optimization of recombinant Yarrowia lipolytica for its efficient succinic acid production[J]. Food and Fermentation Industries, 0, (): 1 .
[7] YUAN Xu, WU Xiao-yu, LI Wei-li, WANG Qing-hui, LIU Ping, LIN Hong-bin, CHE Zhen-ming, WU Tao. Evaluation of nutrition, polyphenols and their antioxidant activities in Pixian bean paste[J]. Food and Fermentation Industries, 2018, 44(9): 270 -274 .
[8] YAO SU,YU Xue-jian,BAI Fei-rong,CAO Yan-hua,ZHAO Ting,ZHAI Lei,LIU Yang,GE Yuan-yuan,CHENG Kun,FENG Hui-jun,LING Kong,SHI Xiao-meng,WANG Yong-fang,ZHANG Xiao-xia,CHENG Chi. Research on the inventory of microbial species in Chinese traditional fermented foods[J]. Food and Fermentation Industries, 2017, 43(9): 238 .
[9] Xia Shuhua,Wang Zhang,Xu Shiying. Application of Macroporots Adsorption Resin in Purification of Bellamya purificata Hydrolysates[J]. Food and Fermentation Industries, 2006, 32(2): 116 .
[10] Xie Yanli,Zhou Huiming,Tan Hongzhuo. Effect of Centrifugal Spray Granulation on the Test Weight and Flow Property of Vitamin a Microcapsule[J]. Food and Fermentation Industries, 2006, 32(4): 9 .
Viewed
Full text


Abstract

Cited

  Shared   
  Discussed   
版权所有 © 《食品与发酵工业》编辑部
地址:北京朝阳区酒仙桥中路24号院6号楼111室
本系统由北京玛格泰克科技发展有限公司设计开发  技术支持:support@magtech.com.cn